Gene description for RTKN
Gene name rhotekin
Gene symbol RTKN
Other names/aliases -
Species Homo sapiens
 Database cross references - RTKN
ExoCarta ExoCarta_6242
Vesiclepedia VP_6242
Entrez Gene 6242
HGNC 10466
MIM 602288
UniProt Q9BST9  
 RTKN identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RTKN
Molecular Function
    GTPase inhibitor activity GO:0005095 IBA
    GTPase inhibitor activity GO:0005095 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    small GTPase binding GO:0031267 IDA
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    actomyosin contractile ring assembly GO:0000915 IBA
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 IDA
    Rho protein signal transduction GO:0007266 IDA
    septin ring organization GO:0031106 IBA
    regulation of apoptotic process GO:0042981 IDA
Subcellular Localization
    actomyosin contractile ring GO:0005826 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified RTKN in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RTKN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC4  
Affinity Capture-MS Homo sapiens
2 KSR1  
Affinity Capture-MS Homo sapiens
3 RALGPS2 55103
Affinity Capture-MS Homo sapiens
4 FAM57B  
Affinity Capture-MS Homo sapiens
5 ANAPC15  
Affinity Capture-MS Homo sapiens
6 TMEM147 10430
Affinity Capture-MS Homo sapiens
7 NAV1 89796
Affinity Capture-MS Homo sapiens
8 CDC25B 994
Affinity Capture-MS Homo sapiens
9 SRGAP2 23380
Affinity Capture-MS Homo sapiens
10 CAMSAP2  
Affinity Capture-MS Homo sapiens
11 CBY1  
Affinity Capture-MS Homo sapiens
12 DNAI2  
Affinity Capture-MS Homo sapiens
13 EIF4E2  
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 KIF13B 23303
Affinity Capture-MS Homo sapiens
16 CAPZB 832
Affinity Capture-MS Homo sapiens
17 TAX1BP3 30851
Affinity Capture-MS Homo sapiens
18 ZBTB21  
Affinity Capture-MS Homo sapiens
19 RASAL2 9462
Affinity Capture-MS Homo sapiens
20 ZNF638 27332
Affinity Capture-MS Homo sapiens
21 FAM110A  
Affinity Capture-MS Homo sapiens
22 TIAM1  
Affinity Capture-MS Homo sapiens
23 PHLDB2 90102
Affinity Capture-MS Homo sapiens
24 PTPN14 5784
Affinity Capture-MS Homo sapiens
25 LRFN1  
Affinity Capture-MS Homo sapiens
26 FEM1A  
Affinity Capture-MS Homo sapiens
27 KIAA1804  
Affinity Capture-MS Homo sapiens
28 MELK  
Affinity Capture-MS Homo sapiens
29 YWHAB 7529
Affinity Capture-MS Homo sapiens
30 AGAP1  
Affinity Capture-MS Homo sapiens
31 ACOT11 26027
Affinity Capture-MS Homo sapiens
32 RHOA 387
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
33 CDK16 5127
Affinity Capture-MS Homo sapiens
34 SULT1C4  
Affinity Capture-MS Homo sapiens
35 ANKRD34A  
Affinity Capture-MS Homo sapiens
36 USP21  
Affinity Capture-MS Homo sapiens
37 B4GALT2  
Affinity Capture-MS Homo sapiens
38 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
39 TESK2  
Affinity Capture-MS Homo sapiens
40 NAA30 122830
Affinity Capture-MS Homo sapiens
41 HDAC7  
Affinity Capture-MS Homo sapiens
42 LPIN3 64900
Affinity Capture-MS Homo sapiens
43 BICD2 23299
Proximity Label-MS Homo sapiens
44 FAM110B  
Affinity Capture-MS Homo sapiens
45 TANC2  
Affinity Capture-MS Homo sapiens
46 KCTD3  
Affinity Capture-MS Homo sapiens
47 LY86  
Affinity Capture-MS Homo sapiens
48 LARP1 23367
Affinity Capture-MS Homo sapiens
49 LIMA1 51474
Affinity Capture-MS Homo sapiens
50 KIF1B 23095
Affinity Capture-MS Homo sapiens
51 DCLK1 9201
Affinity Capture-MS Homo sapiens
52 XPO1 7514
Affinity Capture-MS Homo sapiens
53 OSBPL6  
Affinity Capture-MS Homo sapiens
54 INPP5E 56623
Affinity Capture-MS Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
56 AKAP13 11214
Reconstituted Complex Homo sapiens
57 NADK 65220
Affinity Capture-MS Homo sapiens
58 METTL21B  
Affinity Capture-MS Homo sapiens
59 SYDE1 85360
Affinity Capture-MS Homo sapiens
60 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
61 CDC25C  
Affinity Capture-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 GIGYF1  
Affinity Capture-MS Homo sapiens
64 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
65 CLASRP  
Affinity Capture-MS Homo sapiens
66 RAB11FIP2  
Affinity Capture-MS Homo sapiens
67 CGN  
Affinity Capture-MS Homo sapiens
68 RHOB 388
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
69 DENND4C 55667
Affinity Capture-MS Homo sapiens
70 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
71 SH3RF3  
Affinity Capture-MS Homo sapiens
72 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
73 GIGYF2 26058
Affinity Capture-MS Homo sapiens
74 STARD13 90627
Affinity Capture-MS Homo sapiens
75 NSUN5P1  
Affinity Capture-MS Homo sapiens
76 PARP1 142
Proximity Label-MS Homo sapiens
77 FAM53C 51307
Affinity Capture-MS Homo sapiens
78 RHOC 389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DENND1A 57706
Affinity Capture-MS Homo sapiens
80 PPM1H  
Affinity Capture-MS Homo sapiens
81 KLHDC3  
Affinity Capture-MS Homo sapiens
82 SRSF12  
Affinity Capture-MS Homo sapiens
83 RPTOR 57521
Affinity Capture-MS Homo sapiens
84 TRMT61B  
Affinity Capture-MS Homo sapiens
85 ADO  
Affinity Capture-MS Homo sapiens
86 MAGI1  
Affinity Capture-MS Homo sapiens
87 GAB2 9846
Affinity Capture-MS Homo sapiens
88 NF1 4763
Affinity Capture-MS Homo sapiens
89 MAP1LC3A 84557
Reconstituted Complex Homo sapiens
90 DENND4A 10260
Affinity Capture-MS Homo sapiens
91 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
92 Tnpo1 238799
Affinity Capture-MS Mus musculus
93 KIF1C 10749
Affinity Capture-MS Homo sapiens
94 MAST3  
Affinity Capture-MS Homo sapiens
95 PTPN13 5783
Affinity Capture-MS Homo sapiens
96 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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