Gene description for Tnpo1
Gene name transportin 1
Gene symbol Tnpo1
Other names/aliases AU021749
D13Ertd688e
IPO2
Kpnb2
MIP
MIP1
TRN
Species Mus musculus
 Database cross references - Tnpo1
ExoCarta ExoCarta_238799
Vesiclepedia VP_238799
Entrez Gene 238799
UniProt Q8BFY9  
 Tnpo1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tnpo1
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    small GTPase binding GO:0031267 IEA
    nuclear import signal receptor activity GO:0061608 IBA
Biological Process
    protein import into nucleus GO:0006606 IBA
    protein import into nucleus GO:0006606 IEA
    protein import into nucleus GO:0006606 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Tnpo1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tnpo1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Affinity Capture-MS Homo sapiens
2 Ireb2  
Co-fractionation Mus musculus
3 ATP5H 10476
Affinity Capture-MS Homo sapiens
4 CHCHD6 84303
Affinity Capture-MS Homo sapiens
5 Lmna 16905
Affinity Capture-MS Mus musculus
6 ALDH3A2 224
Affinity Capture-MS Homo sapiens
7 FAM83B  
Affinity Capture-MS Homo sapiens
8 MRPL11 65003
Affinity Capture-MS Homo sapiens
9 HADHB 3032
Affinity Capture-MS Homo sapiens
10 Fus  
Co-fractionation Mus musculus
11 ZNF768 79724
Affinity Capture-MS Homo sapiens
12 ABCG1  
Affinity Capture-MS Homo sapiens
13 RTKN 6242
Affinity Capture-MS Homo sapiens
14 CCHCR1  
Affinity Capture-MS Homo sapiens
15 TFB2M  
Affinity Capture-MS Homo sapiens
16 CNTLN 54875
Affinity Capture-MS Homo sapiens
17 MRPS27 23107
Affinity Capture-MS Homo sapiens
18 KLF16  
Affinity Capture-MS Homo sapiens
19 AFG3L2 10939
Affinity Capture-MS Homo sapiens
20 ATAD3B 83858
Affinity Capture-MS Homo sapiens
21 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
22 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
23 Ptma  
Co-fractionation Mus musculus
24 SPTLC1 10558
Affinity Capture-MS Homo sapiens
25 ATP5F1 515
Affinity Capture-MS Homo sapiens
26 SRPRB 58477
Affinity Capture-MS Homo sapiens
27 ATXN10 25814
Affinity Capture-MS Homo sapiens
28 TGFB1I1 7041
Affinity Capture-MS Homo sapiens
29 SHMT2 6472
Affinity Capture-MS Homo sapiens
30 ZNF281  
Affinity Capture-MS Homo sapiens
31 TMEM43 79188
Affinity Capture-MS Homo sapiens
32 Txnrd1 50493
Co-fractionation Mus musculus
33 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
34 ATP5L 10632
Affinity Capture-MS Homo sapiens
35 Ppp2ca 19052
Co-fractionation Mus musculus
36 SLC38A5 92745
Affinity Capture-MS Homo sapiens
37 ESYT2 57488
Affinity Capture-MS Homo sapiens
38 YME1L1 10730
Affinity Capture-MS Homo sapiens
39 Ran 19384
Co-fractionation Mus musculus
40 Elavl1 15568
Co-fractionation Mus musculus
41 RGPD5  
Two-hybrid Homo sapiens
42 PYGB 5834
Affinity Capture-MS Homo sapiens
43 PCID2 55795
Affinity Capture-MS Homo sapiens
44 ATP1A1 476
Affinity Capture-MS Homo sapiens
45 CCDC101  
Affinity Capture-MS Homo sapiens
46 YIPF5 81555
Affinity Capture-MS Homo sapiens
47 DDX54 79039
Affinity Capture-MS Homo sapiens
48 Zc3h15 69082
Co-fractionation Mus musculus
49 SLC27A2 11001
Affinity Capture-MS Homo sapiens
50 Eed  
Affinity Capture-MS Mus musculus
51 DNM2 1785
Affinity Capture-MS Homo sapiens
52 LYST 1130
Affinity Capture-MS Homo sapiens
53 IVNS1ABP  
Affinity Capture-MS Homo sapiens
54 SUCLA2 8803
Affinity Capture-MS Homo sapiens
55 Npepps 19155
Co-fractionation Mus musculus
56 PDF  
Affinity Capture-MS Homo sapiens
57 Ipo4 75751
Co-fractionation Mus musculus
58 NDUFB11 54539
Affinity Capture-MS Homo sapiens
59 SEC61B 10952
Affinity Capture-MS Homo sapiens
60 TOP1MT  
Affinity Capture-MS Homo sapiens
61 Foxp3  
Affinity Capture-MS Mus musculus
62 CCDC47 57003
Affinity Capture-MS Homo sapiens
63 Vbp1  
Co-fractionation Mus musculus
View the network image/svg+xml



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