Gene description for Lmna
Gene name lamin A
Gene symbol Lmna
Other names/aliases Dhe
Species Mus musculus
 Database cross references - Lmna
ExoCarta ExoCarta_16905
Vesiclepedia VP_16905
Entrez Gene 16905
UniProt P48678  
 Lmna identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lmna
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    protein binding GO:0005515 IPI
    protein phosphatase 1 binding GO:0008157 ISO
    identical protein binding GO:0042802 ISO
    structural constituent of nuclear lamina GO:0160123 IDA
    structural constituent of nuclear lamina GO:0160123 ISO
Biological Process
    protein import into nucleus GO:0006606 IMP
    nucleus organization GO:0006997 IGI
    nucleus organization GO:0006997 IMP
    nuclear envelope organization GO:0006998 IBA
    nuclear envelope organization GO:0006998 IGI
    nuclear envelope organization GO:0006998 ISO
    nuclear envelope organization GO:0006998 ISS
    nuclear migration GO:0007097 IBA
    muscle organ development GO:0007517 ISO
    muscle organ development GO:0007517 ISS
    protein localization GO:0008104 ISO
    negative regulation of cell population proliferation GO:0008285 ISO
    positive regulation of gene expression GO:0010628 IMP
    establishment of cell polarity GO:0030010 NAS
    regulation of cell migration GO:0030334 IMP
    establishment or maintenance of microtubule cytoskeleton polarity GO:0030951 IMP
    heterochromatin formation GO:0031507 IBA
    regulation of protein stability GO:0031647 IMP
    regulation of telomere maintenance GO:0032204 ISO
    protein localization to nucleus GO:0034504 IMP
    protein localization to nucleus GO:0034504 IMP
    positive regulation of osteoblast differentiation GO:0045669 ISO
    nuclear pore localization GO:0051664 IBA
    ventricular cardiac muscle cell development GO:0055015 IMP
    cellular response to hypoxia GO:0071456 ISS
    negative regulation of mesenchymal cell proliferation GO:0072201 IMP
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IMP
    cellular senescence GO:0090398 ISS
    protein localization to nuclear envelope GO:0090435 IBA
    regulation of protein localization to nucleus GO:1900180 IMP
    negative regulation of cardiac muscle hypertrophy in response to stress GO:1903243 IMP
    negative regulation of adipose tissue development GO:1904178 ISO
    DNA double-strand break attachment to nuclear envelope GO:1990683 ISO
    DNA double-strand break attachment to nuclear envelope GO:1990683 ISS
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 TAS
    lamin filament GO:0005638 IDA
    nuclear lamina GO:0005652 IBA
    nuclear lamina GO:0005652 IDA
    nuclear lamina GO:0005652 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    nucleoplasm GO:0005654 TAS
    nuclear matrix GO:0016363 ISO
    nuclear matrix GO:0016363 ISS
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    nuclear membrane GO:0031965 IDA
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified Lmna in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lmna
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Emd  
Affinity Capture-MS Mus musculus
2 Hnrnph2 56258
Affinity Capture-MS Mus musculus
3 Ppil1  
Affinity Capture-MS Mus musculus
4 Psmb5 19173
Affinity Capture-MS Mus musculus
5 MTHFD2 10797
Affinity Capture-MS Homo sapiens
6 Tor1aip1  
Affinity Capture-MS Mus musculus
7 Rab5c 19345
Affinity Capture-MS Mus musculus
8 Rab6b  
Affinity Capture-MS Mus musculus
9 Arpc1b 11867
Affinity Capture-MS Mus musculus
10 Eif3i 54709
Affinity Capture-MS Mus musculus
11 Ddx3y 26900
Affinity Capture-MS Mus musculus
12 Rab2a 59021
Affinity Capture-MS Mus musculus
13 Sec22b  
Affinity Capture-MS Mus musculus
14 Myh9 17886
Affinity Capture-MS Mus musculus
15 Sun1  
Affinity Capture-MS Mus musculus
16 Strap 20901
Affinity Capture-MS Mus musculus
17 Lbr  
Affinity Capture-MS Mus musculus
18 Larp4b 217980
Affinity Capture-MS Mus musculus
19 Glt25d1  
Affinity Capture-MS Mus musculus
20 LMNB1 4001
Affinity Capture-MS Homo sapiens
21 Kcnma1  
Affinity Capture-MS Mus musculus
22 Rab10 19325
Affinity Capture-MS Mus musculus
23 Syne2  
Affinity Capture-MS Mus musculus
24 Gm5560  
Affinity Capture-MS Mus musculus
25 DCAF8 50717
Affinity Capture-MS Homo sapiens
26 Atxn1  
Affinity Capture-MS Mus musculus
27 Kctd13  
Affinity Capture-MS Mus musculus
28 Cnn3 71994
Affinity Capture-MS Mus musculus
29 Hsp90b1 22027
Affinity Capture-MS Mus musculus
30 Rab7 19349
Affinity Capture-MS Mus musculus
31 Anxa3 11745
Affinity Capture-MS Mus musculus
32 Mtap 66902
Affinity Capture-MS Mus musculus
33 Esd 13885
Affinity Capture-MS Mus musculus
34 Ttn  
Affinity Capture-MS Mus musculus
35 LMNB2 84823
Affinity Capture-MS Homo sapiens
36 Nphp1  
Affinity Capture-MS Mus musculus
37 Lmnb1 16906
Affinity Capture-MS Mus musculus
38 Ddx39b 53817
Affinity Capture-MS Mus musculus
39 Mettl21e  
Affinity Capture-MS Mus musculus
40 Serbp1 66870
Affinity Capture-MS Mus musculus
41 Tmem173  
Proximity Label-MS Mus musculus
42 Pitpnb 56305
Affinity Capture-MS Mus musculus
43 HAUS5 23354
Affinity Capture-MS Homo sapiens
44 Sfn 55948
Affinity Capture-MS Mus musculus
45 Cbx5  
Affinity Capture-MS Mus musculus
46 Trim28 21849
Affinity Capture-MS Mus musculus
47 Rbm10 236732
Affinity Capture-MS Mus musculus
48 Sar1a  
Affinity Capture-MS Mus musculus
49 Rab5a 271457
Affinity Capture-MS Mus musculus
50 Sun2  
Affinity Capture-MS Mus musculus
51 Lemd3  
Affinity Capture-MS Mus musculus
52 Actg1 11465
Affinity Capture-MS Mus musculus
53 Gm15032  
Affinity Capture-MS Mus musculus
54 Trp53bp1  
Dosage Rescue Mus musculus
55 Cbx3 12417
Affinity Capture-MS Mus musculus
56 Hnrnpul1 232989
Affinity Capture-MS Mus musculus
57 Invs  
Affinity Capture-MS Mus musculus
58 Luc7l  
Affinity Capture-MS Mus musculus
59 Top2a 21973
Affinity Capture-MS Mus musculus
60 Hist1h1e 50709
Affinity Capture-MS Mus musculus
61 Tnpo1 238799
Affinity Capture-MS Mus musculus
62 Sstr3  
Proximity Label-MS Mus musculus
63 Eed  
Affinity Capture-MS Mus musculus
64 Ube2i  
Affinity Capture-MS Mus musculus
65 Xrcc6  
Affinity Capture-MS Mus musculus
66 DNAJB1 3337
Affinity Capture-MS Homo sapiens
67 Ik  
Affinity Capture-MS Mus musculus
68 Mlip  
Affinity Capture-Western Mus musculus
69 Gm5414  
Affinity Capture-MS Mus musculus
70 Atg12  
Negative Genetic Mus musculus
71 Ywhag 22628
Affinity Capture-MS Mus musculus
72 Fancd2  
Affinity Capture-MS Mus musculus
73 DSP 1832
Affinity Capture-MS Homo sapiens
74 Lmnb2  
Affinity Capture-MS Mus musculus
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