Gene description for MTHFD2
Gene name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Gene symbol MTHFD2
Other names/aliases NMDMC
Species Homo sapiens
 Database cross references - MTHFD2
ExoCarta ExoCarta_10797
Vesiclepedia VP_10797
Entrez Gene 10797
HGNC 7434
MIM 604887
UniProt P13995  
 MTHFD2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for MTHFD2
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 IMP
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 IBA
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 IDA
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 ISS
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 TAS
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 IBA
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 IDA
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 IMP
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 TAS
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IBA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IDA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IMP
    protein binding GO:0005515 IPI
    phosphate ion binding GO:0042301 IDA
    phosphate ion binding GO:0042301 IMP
Biological Process
    tetrahydrofolate interconversion GO:0035999 IBA
    tetrahydrofolate metabolic process GO:0046653 IDA
    folic acid metabolic process GO:0046655 TAS
Subcellular Localization
    extracellular space GO:0005615 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified MTHFD2 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MTHFD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MACROD1 28992
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 PARP3 10039
Affinity Capture-MS Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 CLPP 8192
Proximity Label-MS Homo sapiens
6 CS 1431
Proximity Label-MS Homo sapiens
7 PRDX5 25824
Co-fractionation Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 Lmna 16905
Affinity Capture-MS Mus musculus
10 CLIC1 1192
Co-fractionation Homo sapiens
11 TSFM 10102
Proximity Label-MS Homo sapiens
12 AUH 549
Proximity Label-MS Homo sapiens
13 MTIF2 4528
Proximity Label-MS Homo sapiens
14 AK2 204
Co-fractionation Homo sapiens
15 AK4 205
Co-fractionation Homo sapiens
16 MINOS1  
Affinity Capture-MS Homo sapiens
17 HRAS 3265
Affinity Capture-MS Homo sapiens
18 KIF14 9928
Affinity Capture-MS Homo sapiens
19 MSRB2  
Co-fractionation Homo sapiens
20 SNUPN 10073
Co-fractionation Homo sapiens
21 MDH2 4191
Proximity Label-MS Homo sapiens
22 ACAA1 30
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PSMD13 5719
Affinity Capture-MS Homo sapiens
24 TUFM 7284
Proximity Label-MS Homo sapiens
25 RFX5 5993
Co-fractionation Homo sapiens
26 Nek2  
Affinity Capture-MS Mus musculus
27 NFATC1 4772
Affinity Capture-MS Homo sapiens
28 DHX29 54505
Co-fractionation Homo sapiens
29 SRP9 6726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 DLD 1738
Affinity Capture-MS Homo sapiens
31 BRD8 10902
Affinity Capture-MS Homo sapiens
32 C6orf203  
Proximity Label-MS Homo sapiens
33 PDHA1 5160
Proximity Label-MS Homo sapiens
34 CUL3 8452
Affinity Capture-MS Homo sapiens
35 NAGK 55577
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 LAMTOR2 28956
Co-fractionation Homo sapiens
37 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
38 COX8A  
Proximity Label-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 TXNDC12 51060
Co-fractionation Homo sapiens
41 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
42 MYC  
Affinity Capture-MS Homo sapiens
43 C21orf33  
Proximity Label-MS Homo sapiens
44 SSBP1 6742
Proximity Label-MS Homo sapiens
45 TFCP2 7024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DUSP13  
Affinity Capture-MS Homo sapiens
47 DHX40  
Co-fractionation Homo sapiens
48 PMPCB 9512
Proximity Label-MS Homo sapiens
49 C2orf73  
Affinity Capture-MS Homo sapiens
50 HEATR3 55027
Affinity Capture-MS Homo sapiens
51 UBP1 7342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MTG2  
Proximity Label-MS Homo sapiens
53 GCC1 79571
Co-fractionation Homo sapiens
54 SERBP1 26135
Affinity Capture-MS Homo sapiens
55 MYH9 4627
Affinity Capture-MS Homo sapiens
56 AARS2  
Proximity Label-MS Homo sapiens
57 PEBP1 5037
Co-fractionation Homo sapiens
58 PREP 5550
Co-fractionation Homo sapiens
59 C1QBP 708
Proximity Label-MS Homo sapiens
60 LDLR 3949
Positive Genetic Homo sapiens
61 CALD1 800
Affinity Capture-MS Homo sapiens
62 UFL1 23376
Affinity Capture-MS Homo sapiens
63 C9orf72  
Affinity Capture-MS Homo sapiens
64 HCCS 3052
Co-fractionation Homo sapiens
65 METTL17  
Proximity Label-MS Homo sapiens
66 RER1 11079
Affinity Capture-MS Homo sapiens
67 LYPLA1 10434
Co-fractionation Homo sapiens
68 MCCC1 56922
Co-fractionation Homo sapiens
69 MYCN  
Affinity Capture-MS Homo sapiens
70 ABHD11 83451
Co-fractionation Homo sapiens
71 NFATC2  
Affinity Capture-MS Homo sapiens
72 SCFD1 23256
Co-fractionation Homo sapiens
73 TMEM70  
Proximity Label-MS Homo sapiens
74 MCUR1 63933
Proximity Label-MS Homo sapiens
75 ACADL 33
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 HUWE1 10075
Affinity Capture-MS Homo sapiens
77 PHGDH 26227
Affinity Capture-MS Homo sapiens
78 PTGR2 145482
Co-fractionation Homo sapiens
79 MTHFD2L 441024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here