Gene description for DHX29
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 29
Gene symbol DHX29
Other names/aliases DDX29
Species Homo sapiens
 Database cross references - DHX29
ExoCarta ExoCarta_54505
Vesiclepedia VP_54505
Entrez Gene 54505
HGNC 15815
MIM 612720
UniProt Q7Z478  
 DHX29 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DHX29
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA helicase activity GO:0003724 IEA
    translation initiation factor activity GO:0003743 IEA
    helicase activity GO:0004386 IBA
    ATP binding GO:0005524 IEA
    translation activator activity GO:0008494 IMP
    ATP hydrolysis activity GO:0016887 IEA
    ribonucleoside triphosphate phosphatase activity GO:0017111 IMP
    ribosomal small subunit binding GO:0043024 IDA
    cadherin binding GO:0045296 HDA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    formation of translation preinitiation complex GO:0001731 IMP
    chromatin remodeling GO:0006338 IEA
    ribosome assembly GO:0042255 IMP
    positive regulation of translational initiation GO:0045948 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    eukaryotic 43S preinitiation complex GO:0016282 IDA
    cytosolic small ribosomal subunit GO:0022627 IDA
 Experiment description of studies that identified DHX29 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHX29
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Rpl35 66489
Affinity Capture-MS Mus musculus
3 MTHFD2 10797
Co-fractionation Homo sapiens
4 CHUK 1147
Co-fractionation Homo sapiens
5 RECQL5  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
6 Eif3a 13669
Affinity Capture-MS Mus musculus
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 POGLUT1 56983
Co-fractionation Homo sapiens
9 SLFN11 91607
Affinity Capture-MS Homo sapiens
10 RPS11 6205
Affinity Capture-MS Homo sapiens
11 RPS20 6224
Cross-Linking-MS (XL-MS) Homo sapiens
12 FTL 2512
Affinity Capture-MS Homo sapiens
13 FBXL6  
Affinity Capture-MS Homo sapiens
14 NAPA 8775
Co-fractionation Homo sapiens
15 DNAJC2 27000
Proximity Label-MS Homo sapiens
16 SRP9 6726
Affinity Capture-MS Homo sapiens
17 PISD  
Two-hybrid Homo sapiens
18 RPL10 6134
Affinity Capture-MS Homo sapiens
19 DNAJC17  
Co-fractionation Homo sapiens
20 G3BP2 9908
Affinity Capture-MS Homo sapiens
21 RPL7L1 285855
Affinity Capture-MS Homo sapiens
22 PAPOLG  
Co-fractionation Homo sapiens
23 EIF3B 8662
Affinity Capture-MS Homo sapiens
24 APEX1 328
Affinity Capture-RNA Homo sapiens
25 FOXM1  
Co-fractionation Homo sapiens
26 RPS27 6232
Co-fractionation Homo sapiens
27 CAPZB 832
Affinity Capture-MS Homo sapiens
28 RPA3 6119
Proximity Label-MS Homo sapiens
29 RPS9 6203
Cross-Linking-MS (XL-MS) Homo sapiens
30 METAP2 10988
Affinity Capture-MS Homo sapiens
31 RPL38 6169
Cross-Linking-MS (XL-MS) Homo sapiens
32 TRIM28 10155
Affinity Capture-MS Homo sapiens
33 CDH1 999
Proximity Label-MS Homo sapiens
34 PSPC1 55269
Affinity Capture-MS Homo sapiens
35 FBL 2091
Proximity Label-MS Homo sapiens
36 RPS10 6204
Co-fractionation Homo sapiens
37 HECTD1 25831
Affinity Capture-MS Homo sapiens
38 NTRK1 4914
Affinity Capture-MS Homo sapiens
39 PABPC5 140886
Affinity Capture-MS Homo sapiens
40 PGK1 5230
Cross-Linking-MS (XL-MS) Homo sapiens
41 Rrbp1  
Affinity Capture-MS Mus musculus
42 DCTN1 1639
Proximity Label-MS Homo sapiens
43 COPS5 10987
Affinity Capture-MS Homo sapiens
44 SIRT6  
Affinity Capture-MS Homo sapiens
45 PRC1 9055
Affinity Capture-MS Homo sapiens
46 Setd2  
Affinity Capture-MS Mus musculus
47 RPA4  
Proximity Label-MS Homo sapiens
48 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HNRNPU 3192
Affinity Capture-MS Homo sapiens
50 POU5F1  
Affinity Capture-MS Homo sapiens
51 CENPT  
Two-hybrid Homo sapiens
52 MYC  
Affinity Capture-MS Homo sapiens
53 OBSL1 23363
Affinity Capture-MS Homo sapiens
54 C1qbp 12261
Affinity Capture-MS Mus musculus
55 EDF1 8721
Co-fractionation Homo sapiens
56 GSPT1 2935
Affinity Capture-MS Homo sapiens
57 CDX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HAUS7  
Co-fractionation Homo sapiens
59 RPS16 6217
Affinity Capture-MS Homo sapiens
60 IFIT2 3433
Co-fractionation Homo sapiens
61 RBMS2 5939
Affinity Capture-MS Homo sapiens
62 EIF3H 8667
Affinity Capture-MS Homo sapiens
63 CKAP5 9793
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which DHX29 is involved
No pathways found





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