Gene description for Rab10
Gene name RAB10, member RAS oncogene family
Gene symbol Rab10
Other names/aliases AW107754
Species Mus musculus
 Database cross references - Rab10
ExoCarta ExoCarta_19325
Vesiclepedia VP_19325
Entrez Gene 19325
UniProt P61027  
 Rab10 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab10
Molecular Function
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    myosin V binding GO:0031489 IBA
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
    GDP-dissociation inhibitor binding GO:0051021 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    intracellular protein transport GO:0006886 TAS
    exocytosis GO:0006887 IBA
    Golgi to plasma membrane transport GO:0006893 ISO
    Golgi to plasma membrane transport GO:0006893 ISS
    axonogenesis GO:0007409 ISO
    axonogenesis GO:0007409 ISS
    vesicle-mediated transport GO:0016192 IMP
    endosomal transport GO:0016197 ISO
    endosomal transport GO:0016197 ISS
    antigen processing and presentation GO:0019882 IEA
    antigen processing and presentation GO:0019882 ISO
    polarized epithelial cell differentiation GO:0030859 ISS
    cellular response to insulin stimulus GO:0032869 IMP
    Golgi to plasma membrane protein transport GO:0043001 ISS
    regulated exocytosis GO:0045055 IEA
    regulated exocytosis GO:0045055 ISO
    establishment of neuroblast polarity GO:0045200 ISO
    establishment of neuroblast polarity GO:0045200 ISS
    endoplasmic reticulum tubular network organization GO:0071786 ISO
    endoplasmic reticulum tubular network organization GO:0071786 ISS
    protein localization to plasma membrane GO:0072659 IMP
    establishment of protein localization to membrane GO:0090150 ISO
    establishment of protein localization to endoplasmic reticulum membrane GO:0097051 ISO
    establishment of protein localization to endoplasmic reticulum membrane GO:0097051 ISS
    protein localization to basolateral plasma membrane GO:1903361 ISS
Subcellular Localization
    exocyst GO:0000145 ISS
    lysosome GO:0005764 IEA
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 ISO
    trans-Golgi network GO:0005802 ISS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cilium GO:0005929 IDA
    cilium GO:0005929 ISO
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    membrane GO:0016020 IBA
    cytoplasmic vesicle membrane GO:0030659 TAS
    phagocytic vesicle membrane GO:0030670 IEA
    synaptic vesicle membrane GO:0030672 ISO
    insulin-responsive compartment GO:0032593 ISO
    insulin-responsive compartment GO:0032593 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
    recycling endosome membrane GO:0055038 IEA
    exocytic vesicle GO:0070382 IEA
    exocytic vesicle GO:0070382 ISO
    endoplasmic reticulum tubular network GO:0071782 ISO
    endoplasmic reticulum tubular network GO:0071782 ISS
    secretory vesicle GO:0099503 IBA
 Experiment description of studies that identified Rab10 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Lmna 16905
Affinity Capture-MS Mus musculus
5 MAPT  
Reconstituted Complex Homo sapiens
6 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Rab10 19325
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Mapt  
Affinity Capture-MS Mus musculus
9 Mical1 171580
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
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