Gene description for Hsp90b1
Gene name heat shock protein 90, beta (Grp94), member 1
Gene symbol Hsp90b1
Other names/aliases ERp99
GRP94
TA-3
Targ2
Tra-1
Tra1
endoplasmin
gp96
Species Mus musculus
 Database cross references - Hsp90b1
ExoCarta ExoCarta_22027
Vesiclepedia VP_22027
Entrez Gene 22027
UniProt P08113  
 Hsp90b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hsp90b1
Molecular Function
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    protein phosphatase inhibitor activity GO:0004864 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    protein phosphatase binding GO:0019903 ISO
    protein folding chaperone GO:0044183 ISO
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IEA
    insulin processing GO:0030070 ISO
    retrograde protein transport, ER to cytosol GO:0030970 IEA
    actin rod assembly GO:0031247 ISO
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 ISS
    negative regulation of apoptotic process GO:0043066 IEA
    cellular response to ATP GO:0071318 ISO
Subcellular Localization
    extracellular region GO:0005576 TAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 ISO
    endoplasmic reticulum lumen GO:0005788 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    smooth endoplasmic reticulum GO:0005790 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    protein-containing complex GO:0032991 ISO
    sarcoplasmic reticulum lumen GO:0033018 IEA
    endoplasmic reticulum chaperone complex GO:0034663 IDA
    melanosome GO:0042470 IEA
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    sperm plasma membrane GO:0097524 IDA
 Experiment description of studies that identified Hsp90b1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hsp90b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Ppib 19035
Co-fractionation Mus musculus
4 Nphp4  
Affinity Capture-MS Mus musculus
5 Lmna 16905
Affinity Capture-MS Mus musculus
6 Kcna3  
Affinity Capture-MS Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 Hspa5 14828
Co-purification Mus musculus
9 Tfe3  
Affinity Capture-MS Mus musculus
10 Gabra1  
Affinity Capture-Western Mus musculus
11 Atg16l1  
Affinity Capture-MS Mus musculus
12 Mllt3  
Affinity Capture-Western Mus musculus
13 App 11820
Affinity Capture-MS Mus musculus
14 Atg12  
Negative Genetic Mus musculus
15 Uba5  
Co-fractionation Mus musculus
16 Fancd2  
Affinity Capture-MS Mus musculus
17 Sdf2l1  
Co-fractionation Mus musculus
18 Mttp  
Co-fractionation Mus musculus
19 Lipc  
Affinity Capture-MS Mus musculus
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