Gene description for Sfn
Gene name stratifin
Gene symbol Sfn
Other names/aliases Er
Mme1
Ywhas
Species Mus musculus
 Database cross references - Sfn
ExoCarta ExoCarta_55948
Vesiclepedia VP_55948
Entrez Gene 55948
UniProt O70456  
 Sfn identified in sEVs derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Mast cells 17486113    
 Gene ontology annotations for Sfn
Molecular Function
    protein binding GO:0005515 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    phosphoserine residue binding GO:0050815 ISO
    phosphoserine residue binding GO:0050815 ISS
    phosphoprotein binding GO:0051219 IPI
    protein sequestering activity GO:0140311 ISO
    protein sequestering activity GO:0140311 ISS
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    release of cytochrome c from mitochondria GO:0001836 IEA
    release of cytochrome c from mitochondria GO:0001836 ISO
    keratinocyte development GO:0003334 IGI
    protein export from nucleus GO:0006611 IDA
    signal transduction GO:0007165 IBA
    protein localization GO:0008104 IBA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 ISO
    regulation of epidermal cell division GO:0010482 IMP
    negative regulation of keratinocyte proliferation GO:0010839 IGI
    regulation of cell-cell adhesion GO:0022407 ISS
    keratinocyte differentiation GO:0030216 IMP
    positive regulation of cell growth GO:0030307 IDA
    keratinization GO:0031424 IMP
    skin development GO:0043588 IGI
    skin development GO:0043588 IMP
    keratinocyte proliferation GO:0043616 IGI
    positive regulation of epidermal cell differentiation GO:0045606 IMP
    positive regulation of cell adhesion GO:0045785 ISS
    negative regulation of innate immune response GO:0045824 ISO
    negative regulation of innate immune response GO:0045824 ISS
    positive regulation of protein export from nucleus GO:0046827 IDA
    regulation of cell cycle GO:0051726 IDA
    establishment of skin barrier GO:0061436 IMP
    stem cell proliferation GO:0072089 IGI
    cAMP/PKA signal transduction GO:0141156 IDA
    negative regulation of protein localization to plasma membrane GO:1903077 ISS
    negative regulation of stem cell proliferation GO:2000647 IGI
Subcellular Localization
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified Sfn in sEVs
1
Experiment ID 73
MISEV standards
EM
Biophysical techniques
HSP70|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Sfn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
3 Lmna 16905
Affinity Capture-MS Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Mapt  
Affinity Capture-Western Mus musculus
6 Tcf3  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Nedd4l 83814
Affinity Capture-Western Mus musculus
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