Gene description for AKAP13
Gene name A kinase (PRKA) anchor protein 13
Gene symbol AKAP13
Other names/aliases AKAP-13
AKAP-Lbc
ARHGEF13
BRX
HA-3
Ht31
LBC
PRKA13
PROTO-LB
PROTO-LBC
c-lbc
p47
Species Homo sapiens
 Database cross references - AKAP13
ExoCarta ExoCarta_11214
Vesiclepedia VP_11214
Entrez Gene 11214
HGNC 371
MIM 604686
UniProt Q12802  
 AKAP13 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AKAP13
Molecular Function
    MAP-kinase scaffold activity GO:0005078 IBA
    MAP-kinase scaffold activity GO:0005078 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
    metal ion binding GO:0046872 IEA
    protein kinase A binding GO:0051018 IDA
    molecular adaptor activity GO:0060090 IDA
Biological Process
    MAPK cascade GO:0000165 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    heart development GO:0007507 ISS
    regulation of Rho protein signal transduction GO:0035023 IBA
    positive regulation of Rho protein signal transduction GO:0035025 IDA
    positive regulation of Rho protein signal transduction GO:0035025 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    cardiac muscle cell differentiation GO:0055007 ISS
    regulation of sarcomere organization GO:0060297 ISS
    bone development GO:0060348 ISS
    adrenergic receptor signaling pathway GO:0071875 IBA
    adrenergic receptor signaling pathway GO:0071875 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cell cortex GO:0005938 IDA
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 HDA
    cortical actin cytoskeleton GO:0030864 IDA
 Experiment description of studies that identified AKAP13 in sEVs
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AKAP13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 MTR 4548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NUDT21 11051
Affinity Capture-MS Homo sapiens
5 ARHGEF18 23370
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TKFC 26007
Affinity Capture-MS Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 GNA12 2768
Affinity Capture-Western Homo sapiens
9 PRPF4B 8899
Affinity Capture-MS Homo sapiens
10 SRRM1 10250
Affinity Capture-MS Homo sapiens
11 THRA  
Reconstituted Complex Homo sapiens
12 RTKN 6242
Reconstituted Complex Homo sapiens
13 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LINC00839  
Protein-RNA Homo sapiens
15 C15orf59  
Affinity Capture-MS Homo sapiens
16 PFN1 5216
Proximity Label-MS Homo sapiens
17 YWHAE 7531
Affinity Capture-MS Homo sapiens
18 PRKAR2A 5576
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
19 APC  
Affinity Capture-MS Homo sapiens
20 GNAQ 2776
Affinity Capture-Western Homo sapiens
21 PRKD1 5587
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 Prkca  
Reconstituted Complex Rattus norvegicus
25 PRKCH 5583
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 CTDP1 9150
Affinity Capture-MS Homo sapiens
27 MTA1 9112
Affinity Capture-MS Homo sapiens
28 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PCMT1 5110
Affinity Capture-MS Homo sapiens
30 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 APP 351
Reconstituted Complex Homo sapiens
32 ESR1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
33 BAK1 578
Affinity Capture-MS Homo sapiens
34 MYCN  
Affinity Capture-MS Homo sapiens
35 PRKACA 5566
Biochemical Activity Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MAP2K3 5606
Co-fractionation Homo sapiens
37 MAP1LC3A 84557
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 DDX42 11325
Affinity Capture-MS Homo sapiens
39 MYC  
Affinity Capture-MS Homo sapiens
40 PPARA  
Reconstituted Complex Homo sapiens
41 PRDX2 7001
Affinity Capture-MS Homo sapiens
42 PRKCA 5578
Affinity Capture-Western Homo sapiens
43 HARS2 23438
Affinity Capture-MS Homo sapiens
44 CCNT2  
Affinity Capture-MS Homo sapiens
45 FBXW7  
Affinity Capture-MS Homo sapiens
46 YWHAQ 10971
Affinity Capture-MS Homo sapiens
47 GALM 130589
Affinity Capture-MS Homo sapiens
48 RHOB 388
Proximity Label-MS Homo sapiens
49 RXRB 6257
Reconstituted Complex Homo sapiens
50 YWHAG 7532
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
51 ANXA2 302
Affinity Capture-MS Homo sapiens
52 CCNT1  
Affinity Capture-MS Homo sapiens
53 RHOA 387
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 Cenpe  
Affinity Capture-MS Mus musculus
55 Prkar2a 19087
Affinity Capture-MS Mus musculus
56 Prkacb 18749
Affinity Capture-MS Mus musculus
57 RANBP2 5903
Two-hybrid Homo sapiens
58 Prkch  
Reconstituted Complex Rattus norvegicus
59 CTNNAL1 8727
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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