Gene description for PRKCH
Gene name protein kinase C, eta
Gene symbol PRKCH
Other names/aliases PKC-L
PKCL
PRKCL
nPKC-eta
Species Homo sapiens
 Database cross references - PRKCH
ExoCarta ExoCarta_5583
Vesiclepedia VP_5583
Entrez Gene 5583
HGNC 9403
MIM 605437
UniProt P24723  
 PRKCH identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Urine 19056867    
 Gene ontology annotations for PRKCH
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme binding GO:0019899 IPI
    protein serine/threonine kinase inhibitor activity GO:0030291 IDA
    small GTPase binding GO:0031267 IEA
    translation regulator activity GO:0045182 IDA
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
    protein serine/threonine kinase binding GO:0120283 IPI
Biological Process
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    signal transduction GO:0007165 TAS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 ISS
    negative regulation of translation GO:0017148 IDA
    negative regulation of translation GO:0017148 IMP
    cell differentiation GO:0030154 IEA
    positive regulation of translation in response to stress GO:0032056 IDA
    cellular response to amino acid starvation GO:0034198 IDA
    negative regulation of glial cell apoptotic process GO:0034351 IMP
    intracellular signal transduction GO:0035556 IBA
    positive regulation of keratinocyte differentiation GO:0045618 ISS
    positive regulation of B cell receptor signaling pathway GO:0050861 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    positive regulation of glial cell proliferation GO:0060252 IMP
    protein kinase C signaling GO:0070528 IEA
    negative regulation of protein serine/threonine kinase activity GO:0071901 IDA
    positive regulation of protein localization to plasma membrane GO:1903078 TAS
    regulation of bicellular tight junction assembly GO:2000810 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PRKCH in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKCH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSK3A 2931
Biochemical Activity Homo sapiens
2 BANP  
Two-hybrid Homo sapiens
3 OCIAD1 54940
Proximity Label-MS Homo sapiens
4 AKAP1 8165
Proximity Label-MS Homo sapiens
5 ZBTB8OS 339487
Two-hybrid Homo sapiens
6 PPP1R14A 94274
Reconstituted Complex Homo sapiens
7 CDK2 1017
Affinity Capture-Western Homo sapiens
8 MTCH2 23788
Proximity Label-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 FIS1 51024
Proximity Label-MS Homo sapiens
11 RHOB 388
Proximity Label-MS Homo sapiens
12 ARF1 375
Proximity Label-MS Homo sapiens
13 AKAP13 11214
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 VHL  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 PRKCE 5581
Affinity Capture-MS Homo sapiens
16 PRKCA 5578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
18 RIPK4  
Affinity Capture-MS Homo sapiens
19 MGMT 4255
Affinity Capture-MS Homo sapiens
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