Gene description for PRKCA
Gene name protein kinase C, alpha
Gene symbol PRKCA
Other names/aliases AAG6
PKC-alpha
PKCA
PRKACA
Species Homo sapiens
 Database cross references - PRKCA
ExoCarta ExoCarta_5578
Vesiclepedia VP_5578
Entrez Gene 5578
HGNC 9393
MIM 176960
UniProt P17252  
 PRKCA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for PRKCA
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 EXP
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IDA
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IMP
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 ISS
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    integrin binding GO:0005178 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IEA
    enzyme binding GO:0019899 IDA
    diacylglycerol binding GO:0019992 TAS
    histone H3T6 kinase activity GO:0035403 IDA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IMP
    desmosome assembly GO:0002159 IMP
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    mitotic nuclear membrane disassembly GO:0007077 TAS
    cell adhesion GO:0007155 IEA
    positive regulation of endothelial cell migration GO:0010595 IMP
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISS
    peptidyl-threonine phosphorylation GO:0018107 ISS
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    positive regulation of insulin secretion GO:0032024 TAS
    negative regulation of glial cell apoptotic process GO:0034351 IMP
    intracellular signal transduction GO:0035556 IBA
    regulation of mRNA stability GO:0043488 TAS
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    post-translational protein modification GO:0043687 IDA
    positive regulation of macrophage differentiation GO:0045651 ISS
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of bone resorption GO:0045780 ISS
    positive regulation of cell adhesion GO:0045785 IMP
    positive regulation of mitotic cell cycle GO:0045931 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    response to interleukin-1 GO:0070555 IMP
    regulation of platelet aggregation GO:0090330 IDA
    apoptotic signaling pathway GO:0097190 TAS
    positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0106071 ISS
    positive regulation of angiotensin-activated signaling pathway GO:0110063 IDA
    positive regulation of dense core granule biogenesis GO:2000707 ISS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    mitochondrial membrane GO:0031966 IEA
    alphav-beta3 integrin-PKCalpha complex GO:0035866 ISS
    ciliary basal body GO:0036064 IDA
    perinuclear region of cytoplasm GO:0048471 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PRKCA in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 226
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
12
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 235
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
16
Experiment ID 236
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
17
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
18
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKCA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 EIF4B 1975
Co-fractionation Homo sapiens
3 BPHL 670
Affinity Capture-MS Homo sapiens
4 DPPA3  
Affinity Capture-MS Homo sapiens
5 MAPK3 5595
Co-fractionation Homo sapiens
6 UBL4A 8266
Affinity Capture-MS Homo sapiens
7 GABRB3 2562
Reconstituted Complex Homo sapiens
8 WWC1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 SACM1L 22908
Two-hybrid Homo sapiens
10 RGS2  
Biochemical Activity Homo sapiens
11 AKAP12 9590
Affinity Capture-Western Homo sapiens
12 MAPK1 5594
Co-fractionation Homo sapiens
13 ERCC8  
Affinity Capture-MS Homo sapiens
14 CD53 963
Affinity Capture-Western Homo sapiens
15 FBXO25  
Biochemical Activity Homo sapiens
16 FGD4 121512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HTATIP2 10553
Co-fractionation Homo sapiens
18 UQCRB 7381
Affinity Capture-MS Homo sapiens
19 ATM 472
Synthetic Lethality Homo sapiens
20 ANXA6 309
Co-purification Homo sapiens
21 CLUH 23277
Co-fractionation Homo sapiens
22 NCF1  
Biochemical Activity Homo sapiens
23 GSK3A 2931
Biochemical Activity Homo sapiens
24 SEPT9 10801
Affinity Capture-MS Homo sapiens
25 TOP2A 7153
Biochemical Activity Homo sapiens
26 AFAP1 60312
Reconstituted Complex Homo sapiens
27 PLCG2 5336
Affinity Capture-Western Homo sapiens
28 LIPT1  
Affinity Capture-MS Homo sapiens
29 INSRR 3645
Affinity Capture-MS Homo sapiens
30 GTF2E2  
Affinity Capture-MS Homo sapiens
31 EP300 2033
Biochemical Activity Homo sapiens
32 PITPNB 23760
Affinity Capture-MS Homo sapiens
33 KLC2 64837
Co-fractionation Homo sapiens
34 GNS 2799
Co-fractionation Homo sapiens
35 KIF5B 3799
Co-fractionation Homo sapiens
36 DUSP11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SCRIB 23513
Biochemical Activity Homo sapiens
38 ADD1 118
Biochemical Activity Homo sapiens
39 YWHAG 7532
Affinity Capture-Western Homo sapiens
40 TNP2  
Biochemical Activity Homo sapiens
41 TIAM1  
Biochemical Activity Homo sapiens
42 HMGA1 3159
Biochemical Activity Homo sapiens
43 GSPT1 2935
Co-fractionation Homo sapiens
44 NR1H2  
Biochemical Activity Homo sapiens
45 PLD2 5338
Affinity Capture-Western Homo sapiens
46 JSRP1  
Affinity Capture-MS Homo sapiens
47 PPM1A 5494
Biochemical Activity Homo sapiens
48 AVPR1B  
Affinity Capture-Western Homo sapiens
49 TAS2R7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 PSMB6 5694
Co-fractionation Homo sapiens
51 POLR1E  
Co-fractionation Homo sapiens
52 GRM5  
Biochemical Activity Homo sapiens
53 PSAP 5660
Co-fractionation Homo sapiens
54 STARD7 56910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 DYNLT1 6993
Proximity Label-MS Homo sapiens
56 CBL 867
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
57 HSPA8 3312
Co-fractionation Homo sapiens
58 FAS 355
Co-localization Homo sapiens
59 Bcas2  
Affinity Capture-MS Mus musculus
60 TFDP2  
Affinity Capture-MS Homo sapiens
61 TMEM120A 83862
Affinity Capture-MS Homo sapiens
62 TRIM41 90933
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
63 PHF20L1  
Affinity Capture-MS Homo sapiens
64 CYP2S1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 HIST1H1T 3010
Biochemical Activity Homo sapiens
66 GJA1 2697
Affinity Capture-Western Homo sapiens
67 CCR3  
Affinity Capture-MS Homo sapiens
68 PARK2  
Affinity Capture-MS Homo sapiens
69 PLA2G4A 5321
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
70 PRKCB 5579
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 Grin2c  
Biochemical Activity Rattus norvegicus
72 PLD1 5337
Affinity Capture-Western Homo sapiens
73 IL23R  
Affinity Capture-MS Homo sapiens
74 ACIN1 22985
Co-fractionation Homo sapiens
75 PRKCG 5582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CERS2 29956
Affinity Capture-MS Homo sapiens
77 APLP2 334
Biochemical Activity Homo sapiens
78 HIST1H1D 3007
Biochemical Activity Homo sapiens
79 MAPT  
Biochemical Activity Homo sapiens
80 Ctnnbl1  
Affinity Capture-MS Mus musculus
81 MKRN2 23609
Affinity Capture-RNA Homo sapiens
82 NUMB 8650
Affinity Capture-MS Homo sapiens
83 NTRK1 4914
Affinity Capture-MS Homo sapiens
84 HAND1  
Biochemical Activity Homo sapiens
85 MGMT 4255
Biochemical Activity Homo sapiens
86 HIST1H1E 3008
Biochemical Activity Homo sapiens
87 HMGA2 8091
Biochemical Activity Homo sapiens
88 SRSF9 8683
Co-fractionation Homo sapiens
89 RDH11 51109
Affinity Capture-MS Homo sapiens
90 GRIN1 2902
Biochemical Activity Homo sapiens
91 KIF11 3832
Affinity Capture-MS Homo sapiens
92 HIST1H1C 3006
Biochemical Activity Homo sapiens
93 NOXA1  
Biochemical Activity Homo sapiens
94 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
95 SHCBP1 79801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 EIF4EBP1  
Biochemical Activity Homo sapiens
97 ITGB2 3689
Biochemical Activity Homo sapiens
98 CFHR3 10878
Affinity Capture-MS Homo sapiens
99 PPY  
Affinity Capture-MS Homo sapiens
100 CLDN10  
Affinity Capture-MS Homo sapiens
101 TMEM185A  
Affinity Capture-MS Homo sapiens
102 PTGIR  
Biochemical Activity Homo sapiens
103 TRPV6  
Biochemical Activity Homo sapiens
104 ANLN 54443
Affinity Capture-MS Homo sapiens
105 HABP4  
Biochemical Activity Homo sapiens
106 PICK1  
Affinity Capture-Western Homo sapiens
107 PER2  
Proximity Label-MS Homo sapiens
108 CYP3A4  
Biochemical Activity Homo sapiens
109 HIST1H3A 8350
Biochemical Activity Homo sapiens
110 VPS4B 9525
Affinity Capture-MS Homo sapiens
111 MUS81  
Affinity Capture-MS Homo sapiens
112 YWHAZ 7534
Affinity Capture-Western Homo sapiens
113 LMNB1 4001
Reconstituted Complex Homo sapiens
114 GLI3 2737
Biochemical Activity Homo sapiens
115 EIF2S1 1965
Biochemical Activity Homo sapiens
116 PPARG 5468
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
117 CTNNB1 1499
Affinity Capture-Western Homo sapiens
118 CCDC8  
Affinity Capture-MS Homo sapiens
119 TFAP4 7023
Affinity Capture-MS Homo sapiens
120 RICTOR 253260
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
121 CD9 928
Affinity Capture-Western Homo sapiens
122 AKAP13 11214
Affinity Capture-Western Homo sapiens
123 DLG4  
Affinity Capture-Western Homo sapiens
124 TAS2R20  
Affinity Capture-MS Homo sapiens
125 FTH1 2495
Affinity Capture-MS Homo sapiens
126 PIGK 10026
Affinity Capture-MS Homo sapiens
127 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 HIST3H3 8290
Biochemical Activity Homo sapiens
129 TNP1  
Biochemical Activity Homo sapiens
130 NXF1 10482
Affinity Capture-RNA Homo sapiens
131 AVPR1A 552
Affinity Capture-Western Homo sapiens
132 NF2 4771
Biochemical Activity Homo sapiens
133 PSMB4 5692
Two-hybrid Homo sapiens
134 GRM7 2917
Affinity Capture-Western Homo sapiens
135 HIST1H1B 3009
Biochemical Activity Homo sapiens
136 CCNL2 81669
Affinity Capture-MS Homo sapiens
137 RPA3 6119
Proximity Label-MS Homo sapiens
138 GRIN2B  
Biochemical Activity Homo sapiens
139 AKAP5  
Affinity Capture-Western Homo sapiens
140 EGFR 1956
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
141 MTOR 2475
Biochemical Activity Homo sapiens
142 HAND2  
Biochemical Activity Homo sapiens
143 HSPA1A 3303
Co-fractionation Homo sapiens
144 HIST1H1A 3024
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
145 FCGR2B  
Reconstituted Complex Homo sapiens
146 ISOC1 51015
Affinity Capture-MS Homo sapiens
147 TRNT1 51095
Affinity Capture-MS Homo sapiens
148 FSCN1 6624
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
149 LMNA 4000
Protein-peptide Homo sapiens
150 PEX3 8504
Affinity Capture-MS Homo sapiens
151 CHUK 1147
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
152 FAM133A 286499
Affinity Capture-MS Homo sapiens
153 DSP 1832
Co-fractionation Homo sapiens
154 PFKFB1  
Biochemical Activity Homo sapiens
155 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 RALBP1 10928
Biochemical Activity Homo sapiens
157 KIF2A 3796
Affinity Capture-MS Homo sapiens
158 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
159 SLC25A11 8402
Affinity Capture-MS Homo sapiens
160 MAPKAP1 79109
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
161 LNX2  
Affinity Capture-Western Homo sapiens
162 UBA5 79876
Affinity Capture-MS Homo sapiens
163 PDE7A  
Affinity Capture-MS Homo sapiens
164 OPALIN  
Affinity Capture-MS Homo sapiens
165 Marcks 17118
Biochemical Activity Mus musculus
166 SLK 9748
Co-fractionation Homo sapiens
167 HDAC6 10013
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
168 PSMB7 5695
Co-fractionation Homo sapiens
169 EZR 7430
Affinity Capture-Western Homo sapiens
170 SCP2 6342
Affinity Capture-MS Homo sapiens
171 WWC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
172 ALS2CR12  
Affinity Capture-MS Homo sapiens
173 SMURF1 57154
Biochemical Activity Homo sapiens
174 PRKCH 5583
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 GCLM 2730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
176 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
177 ZDHHC23  
Affinity Capture-MS Homo sapiens
178 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
179 HR  
Biochemical Activity Homo sapiens
180 DDX58 23586
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
181 VTN 7448
Biochemical Activity Homo sapiens
182 KLF5  
Biochemical Activity Homo sapiens
183 MBP 4155
Biochemical Activity Homo sapiens
184 PPP1R14A 94274
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
185 OGG1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
186 MAPK7  
Co-fractionation Homo sapiens
187 GSK3B 2932
Biochemical Activity Homo sapiens
188 WWC3 55841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
189 MOV10 4343
Affinity Capture-RNA Homo sapiens
190 RNF31 55072
Biochemical Activity Homo sapiens
191 ARMS2 387715
Affinity Capture-MS Homo sapiens
192 IBTK 25998
Affinity Capture-Western Homo sapiens
193 PRKCA 5578
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
194 ATP1A1 476
Biochemical Activity Homo sapiens
195 TUBD1  
Affinity Capture-MS Homo sapiens
196 TRIB3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
197 C1QBP 708
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
198 SERBP1 26135
Affinity Capture-MS Homo sapiens
199 FBXO21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
200 GNB2L1 10399
Affinity Capture-Western Homo sapiens
201 TMEM53  
Affinity Capture-MS Homo sapiens
202 SDC2 6383
Biochemical Activity Homo sapiens
203 SLC1A1 6505
Affinity Capture-Western Homo sapiens
204 SELL 6402
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
205 IKBKB 3551
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
206 RBCK1  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
207 TBXA2R 6915
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
208 LNX1  
Affinity Capture-Western Homo sapiens
209 CDKN2A 1029
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PRKCA is involved
PathwayEvidenceSource
Acetylcholine regulates insulin secretion TAS Reactome
Axon guidance TAS Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling TAS Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca-dependent events TAS Reactome
Ca2+ pathway IEA Reactome
Calmodulin induced events TAS Reactome
CaM pathway TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
DAG and IP3 signaling TAS Reactome
Depolymerization of the Nuclear Lamina TAS Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disinhibition of SNARE formation TAS Reactome
EGFR Transactivation by Gastrin TAS Reactome
Extracellular matrix organization TAS Reactome
G alpha (i) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G alpha (z) signalling events IEA Reactome
G-protein mediated events TAS Reactome
Gastrin-CREB signalling pathway via PKC and MAPK TAS Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity TAS Reactome
GPCR downstream signalling IEA Reactome
GPCR downstream signalling TAS Reactome
Hemostasis TAS Reactome
HuR (ELAVL1) binds and stabilizes mRNA TAS Reactome
Inactivation, recovery and regulation of the phototransduction cascade IEA Reactome
Integration of energy metabolism TAS Reactome
Intracellular signaling by second messengers TAS Reactome
M Phase TAS Reactome
Metabolism TAS Reactome
Metabolism of RNA TAS Reactome
Mitotic Prophase TAS Reactome
Nervous system development TAS Reactome
Nervous system development IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Non-integrin membrane-ECM interactions TAS Reactome
Nuclear Envelope Breakdown TAS Reactome
Opioid Signalling TAS Reactome
PCP/CE pathway TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
PLC beta mediated events TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of KIT signaling TAS Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
RET signaling TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate NADPH Oxidases TAS Reactome
ROBO receptors bind AKAP5 TAS Reactome
ROBO receptors bind AKAP5 IEA Reactome
Sensory Perception IEA Reactome
SHC1 events in ERBB2 signaling TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by ERBB2 TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by ROBO receptors TAS Reactome
Signaling by ROBO receptors IEA Reactome
Signaling by SCF-KIT TAS Reactome
Signaling by VEGF TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Syndecan interactions TAS Reactome
The phototransduction cascade IEA Reactome
Trafficking of AMPA receptors TAS Reactome
Trafficking of GluR2-containing AMPA receptors TAS Reactome
Transmission across Chemical Synapses TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
VEGFR2 mediated cell proliferation TAS Reactome
Visual phototransduction IEA Reactome
WNT5A-dependent internalization of FZD4 TAS Reactome





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