Gene description for TRNT1
Gene name tRNA nucleotidyl transferase, CCA-adding, 1
Gene symbol TRNT1
Other names/aliases CCA1
CGI-47
MtCCA
SIFD
Species Homo sapiens
 Database cross references - TRNT1
ExoCarta ExoCarta_51095
Vesiclepedia VP_51095
Entrez Gene 51095
HGNC 17341
MIM 612907
UniProt Q96Q11  
 TRNT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TRNT1
Molecular Function
    tRNA binding GO:0000049 IBA
    tRNA binding GO:0000049 IDA
    CCA tRNA nucleotidyltransferase activity GO:0004810 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
    5'-3' RNA polymerase activity GO:0034062 TAS
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    CCACCA tRNA nucleotidyltransferase activity GO:0160016 IDA
Biological Process
    tRNA 3'-terminal CCA addition GO:0001680 IBA
    tRNA 3'-terminal CCA addition GO:0001680 IDA
    tRNA 3'-end processing GO:0042780 TAS
    rescue of stalled ribosome GO:0072344 IDA
    tRNA surveillance GO:0106354 IDA
    mitochondrial tRNA 3'-end processing GO:1990180 IBA
    mitochondrial tRNA 3'-end processing GO:1990180 IDA
    mitochondrial tRNA 3'-end processing GO:1990180 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified TRNT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TRNT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GOLGA4  
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 OGFOD1  
Co-fractionation Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 MYCN  
Affinity Capture-MS Homo sapiens
6 CPOX 1371
Co-fractionation Homo sapiens
7 MPRIP 23164
Affinity Capture-MS Homo sapiens
8 RAD23A 5886
Co-fractionation Homo sapiens
9 TP53 7157
Negative Genetic Homo sapiens
10 TRMT10C 54931
Affinity Capture-MS Homo sapiens
11 PYGL 5836
Co-fractionation Homo sapiens
12 LIG4 3981
Cross-Linking-MS (XL-MS) Homo sapiens
13 ANXA3 306
Co-fractionation Homo sapiens
14 PPFIA2 8499
Cross-Linking-MS (XL-MS) Homo sapiens
15 H2AFZ 3015
Co-fractionation Homo sapiens
16 USP5 8078
Co-fractionation Homo sapiens
17 EZR 7430
Co-fractionation Homo sapiens
18 MEN1 4221
Affinity Capture-MS Homo sapiens
19 NTNG1  
Affinity Capture-MS Homo sapiens
20 EGFR 1956
Negative Genetic Homo sapiens
21 BASP1 10409
Co-fractionation Homo sapiens
22 CLPP 8192
Proximity Label-MS Homo sapiens
23 TXNDC17 84817
Co-fractionation Homo sapiens
24 DPYD 1806
Co-fractionation Homo sapiens
25 CTBP2 1488
Co-fractionation Homo sapiens
26 CLNS1A 1207
Co-fractionation Homo sapiens
27 ACACB 32
Cross-Linking-MS (XL-MS) Homo sapiens
28 KNTC1 9735
Cross-Linking-MS (XL-MS) Homo sapiens
29 EPSTI1  
Affinity Capture-MS Homo sapiens
30 MC1R 4157
Co-fractionation Homo sapiens
31 ACOT13  
Co-fractionation Homo sapiens
32 CTSA 5476
Co-fractionation Homo sapiens
33 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
34 MYC  
Affinity Capture-MS Homo sapiens
35 SELENBP1 8991
Affinity Capture-MS Homo sapiens
36 PRKCA 5578
Affinity Capture-MS Homo sapiens
37 H2AFV 94239
Co-fractionation Homo sapiens
38 TBCE 6905
Co-fractionation Homo sapiens
39 NARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MTX3  
Affinity Capture-MS Homo sapiens
41 UBA6 55236
Co-fractionation Homo sapiens
42 PPFIA1 8500
Cross-Linking-MS (XL-MS) Homo sapiens
43 MVK 4598
Affinity Capture-MS Homo sapiens
44 GNPDA1 10007
Co-fractionation Homo sapiens
45 MTMR4  
Affinity Capture-MS Homo sapiens
46 UBXN1 51035
Co-fractionation Homo sapiens
47 SH3BP1 23616
Co-fractionation Homo sapiens
48 ATIC 471
Co-fractionation Homo sapiens
49 DOC2A  
Affinity Capture-MS Homo sapiens
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