Gene description for PPFIA2
Gene name
protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Gene symbol
PPFIA2
Other names/aliases
-
Species
Homo sapiens
Database cross references - PPFIA2
ExoCarta
ExoCarta_8499
Vesiclepedia
VP_8499
Entrez Gene
8499
HGNC
9246
MIM
603143
UniProt
O75334
PPFIA2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells
25944692
Urine
19056867
Gene ontology annotations for PPFIA2
Experiment description of studies that identified PPFIA2 in sEVs
1
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
2
Experiment ID
63
MISEV standards
✘
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for PPFIA2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
PPP2CA
5515
Affinity Capture-MS
Homo sapiens
2
PTPRF
5792
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
3
PPFIA2
8499
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
4
ERC2
26059
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
5
PPFIA1
8500
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
6
ITSN1
6453
Two-hybrid
Homo sapiens
7
TRNT1
51095
Cross-Linking-MS (XL-MS)
Homo sapiens
8
BRK1
55845
Affinity Capture-MS
Homo sapiens
9
GIT1
28964
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
10
PPP2R1A
5518
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
11
PTPRS
5802
Two-hybrid
Homo sapiens
12
CDC5L
988
Two-hybrid
Homo sapiens
13
SETD6
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which PPFIA2 is involved