Gene description for PPFIA2
Gene name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Gene symbol PPFIA2
Other names/aliases -
Species Homo sapiens
 Database cross references - PPFIA2
ExoCarta ExoCarta_8499
Vesiclepedia VP_8499
Entrez Gene 8499
HGNC 9246
MIM 603143
UniProt O75334  
 PPFIA2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 19056867    
 Gene ontology annotations for PPFIA2
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of postsynaptic density GO:0098919 IEA
    structural constituent of presynapse GO:0099181 IEA
Biological Process
    cell-matrix adhesion GO:0007160 TAS
    synapse organization GO:0050808 IBA
    regulation of dendritic spine development GO:0060998 IMP
    regulation of dendritic spine morphogenesis GO:0061001 IMP
    presynapse organization GO:0099172 IEA
    dense core granule cytoskeletal transport GO:0099519 IMP
    maintenance of postsynaptic density structure GO:0099562 IEA
    regulation of synaptic vesicle exocytosis GO:2000300 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    synaptic vesicle GO:0008021 IEA
    cell surface GO:0009986 IEA
    postsynaptic density GO:0014069 IEA
    axon GO:0030424 IEA
    presynaptic membrane GO:0042734 IEA
    dendritic spine GO:0043197 IDA
    presynaptic active zone GO:0048786 IBA
    presynaptic active zone GO:0048786 TAS
    extracellular exosome GO:0070062 HDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified PPFIA2 in sEVs
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PPFIA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2CA 5515
Affinity Capture-MS Homo sapiens
2 PTPRF 5792
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 PPFIA2 8499
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 ERC2 26059
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 PPFIA1 8500
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 ITSN1 6453
Two-hybrid Homo sapiens
7 TRNT1 51095
Cross-Linking-MS (XL-MS) Homo sapiens
8 BRK1 55845
Affinity Capture-MS Homo sapiens
9 GIT1 28964
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
11 PTPRS 5802
Two-hybrid Homo sapiens
12 CDC5L 988
Two-hybrid Homo sapiens
13 SETD6  
Affinity Capture-MS Homo sapiens
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