Gene description for AFAP1
Gene name actin filament associated protein 1
Gene symbol AFAP1
Other names/aliases AFAP
AFAP-110
AFAP110
Species Homo sapiens
 Database cross references - AFAP1
ExoCarta ExoCarta_60312
Vesiclepedia VP_60312
Entrez Gene 60312
HGNC 24017
MIM 608252
UniProt Q8N556  
 AFAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AFAP1
Molecular Function
    actin binding GO:0003779 IEA
Biological Process
    regulation of signal transduction GO:0009966 IBA
Subcellular Localization
    stress fiber GO:0001725 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    actin filament GO:0005884 IBA
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IDA
    actin cytoskeleton GO:0015629 IDA
 Experiment description of studies that identified AFAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AFAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TPX2  
Affinity Capture-MS Homo sapiens
3 LIMA1 51474
Affinity Capture-MS Homo sapiens
4 MECP2 4204
Affinity Capture-MS Homo sapiens
5 Myh10 77579
Affinity Capture-MS Mus musculus
6 PPP1CB 5500
Affinity Capture-MS Homo sapiens
7 CSNK2B 1460
Affinity Capture-MS Homo sapiens
8 FGFR1 2260
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 Myh9 17886
Affinity Capture-MS Mus musculus
10 Lima1  
Affinity Capture-MS Mus musculus
11 PRKCD 5580
Reconstituted Complex Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 Tpm1 22003
Affinity Capture-MS Mus musculus
15 ECT2 1894
Affinity Capture-MS Homo sapiens
16 Rab5b 19344
Affinity Capture-MS Mus musculus
17 PRKCA 5578
Reconstituted Complex Homo sapiens
18 TOLLIP 54472
Proximity Label-MS Homo sapiens
19 Calml3  
Affinity Capture-MS Mus musculus
20 Myo1c 17913
Affinity Capture-MS Mus musculus
21 GAK 2580
Affinity Capture-MS Homo sapiens
22 BRCA1 672
Affinity Capture-MS Homo sapiens
23 HIST1H1T 3010
Cross-Linking-MS (XL-MS) Homo sapiens
24 CAPZA2 830
Affinity Capture-MS Homo sapiens
25 MYO19  
Affinity Capture-MS Homo sapiens
26 ACTC1 70
Reconstituted Complex Homo sapiens
27 Tpx2  
Affinity Capture-MS Mus musculus
28 Actb 11461
Affinity Capture-MS Mus musculus
29 MYO5C 55930
Affinity Capture-MS Homo sapiens
30 MYO1C 4641
Affinity Capture-MS Homo sapiens
31 MAPRE1 22919
Affinity Capture-MS Homo sapiens
32 NXF1 10482
Affinity Capture-RNA Homo sapiens
33 PRKCG 5582
Reconstituted Complex Homo sapiens
34 CDK2 1017
Affinity Capture-MS Homo sapiens
35 AFAP1 60312
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
36 SYNPO 11346
Affinity Capture-MS Homo sapiens
37 Flnb 286940
Affinity Capture-MS Mus musculus
38 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MYH9 4627
Affinity Capture-MS Homo sapiens
40 Tmod3 50875
Affinity Capture-MS Mus musculus
41 PRKCE 5581
Reconstituted Complex Homo sapiens
42 FLNA 2316
Affinity Capture-MS Homo sapiens
43 Flot1 14251
Affinity Capture-MS Mus musculus
44 PRC1 9055
Affinity Capture-MS Homo sapiens
45 IQGAP1 8826
Affinity Capture-MS Homo sapiens
46 EXT2 2132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CHMP4B 128866
Affinity Capture-MS Homo sapiens
48 Coro1c 23790
Affinity Capture-MS Mus musculus
49 PRKCB 5579
Reconstituted Complex Homo sapiens
50 Ppp1cb 19046
Affinity Capture-MS Mus musculus
51 Sept9  
Affinity Capture-MS Mus musculus
52 Flot2 14252
Affinity Capture-MS Mus musculus
53 DBN1 1627
Affinity Capture-MS Homo sapiens
54 TNFRSF1B  
Affinity Capture-MS Homo sapiens
55 MYO18A 399687
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AFAP1 is involved
No pathways found





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