Gene description for Rab5b
Gene name RAB5B, member RAS oncogene family
Gene symbol Rab5b
Other names/aliases C030027M18Rik
Species Mus musculus
 Database cross references - Rab5b
ExoCarta ExoCarta_19344
Vesiclepedia VP_19344
Entrez Gene 19344
UniProt P61021  
 Rab5b identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rab5b
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    GTP-dependent protein binding GO:0030742 IEA
    GTP-dependent protein binding GO:0030742 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    endocytosis GO:0006897 IBA
    endosome organization GO:0007032 IDA
    protein transport GO:0015031 IEA
    antigen processing and presentation GO:0019882 IEA
    antigen processing and presentation GO:0019882 ISO
    regulation of endocytosis GO:0030100 IDA
    plasma membrane to endosome transport GO:0048227 IEA
    plasma membrane to endosome transport GO:0048227 ISO
Subcellular Localization
    endosome GO:0005768 ISO
    early endosome GO:0005769 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    endomembrane system GO:0012505 IBA
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 IDA
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 ISO
    synaptic vesicle membrane GO:0030672 ISO
    early endosome membrane GO:0031901 IEA
    melanosome GO:0042470 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Rab5b in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rab5b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF123 63891
Affinity Capture-MS Homo sapiens
2 CLSPN  
Affinity Capture-MS Homo sapiens
3 XPO1 7514
Affinity Capture-MS Homo sapiens
4 LETMD1  
Affinity Capture-MS Homo sapiens
5 RAB5C 5878
Affinity Capture-MS Homo sapiens
6 UHRF1BP1L 23074
Affinity Capture-MS Homo sapiens
7 DEPDC7  
Affinity Capture-MS Homo sapiens
8 TMEM201 199953
Affinity Capture-MS Homo sapiens
9 Vps8  
Co-fractionation Mus musculus
10 SPTLC1 10558
Affinity Capture-MS Homo sapiens
11 Rab7 19349
Co-fractionation Mus musculus
12 ASXL2  
Affinity Capture-MS Homo sapiens
13 AFAP1 60312
Affinity Capture-MS Homo sapiens
14 Rab5a 271457
Co-fractionation Mus musculus
15 Park7 57320
Co-fractionation Mus musculus
16 OSBPL1A 114876
Affinity Capture-MS Homo sapiens
17 KCNAB2 8514
Affinity Capture-MS Homo sapiens
18 Lrrk2  
Co-fractionation Mus musculus
Phenotypic Suppression Mus musculus
19 Fancd2  
Affinity Capture-MS Mus musculus
20 CDC27 996
Affinity Capture-MS Homo sapiens
21 EIF3L 51386
Affinity Capture-MS Homo sapiens
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