Gene description for OSBPL1A
Gene name oxysterol binding protein-like 1A
Gene symbol OSBPL1A
Other names/aliases ORP-1
ORP1
OSBPL1B
Species Homo sapiens
 Database cross references - OSBPL1A
ExoCarta ExoCarta_114876
Vesiclepedia VP_114876
Entrez Gene 114876
HGNC 16398
MIM 606730
UniProt Q9BXW6  
 OSBPL1A identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for OSBPL1A
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 NAS
    cholesterol binding GO:0015485 IBA
    cholesterol binding GO:0015485 IDA
    sterol transfer activity GO:0120015 TAS
Biological Process
    bile acid biosynthetic process GO:0006699 TAS
    cholesterol metabolic process GO:0008203 NAS
    sterol transport GO:0015918 IEA
    vesicle-mediated transport GO:0016192 NAS
Subcellular Localization
    endosome GO:0005768 IDA
    late endosome GO:0005770 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    organelle membrane contact site GO:0044232 IDA
    extracellular exosome GO:0070062 HDA
    perinuclear endoplasmic reticulum GO:0097038 IBA
 Experiment description of studies that identified OSBPL1A in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for OSBPL1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TXLNA 200081
Affinity Capture-MS Homo sapiens
2 POLG2  
Affinity Capture-MS Homo sapiens
3 ZNF331  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ABT1 29777
Affinity Capture-MS Homo sapiens
6 RAB7A 7879
Proximity Label-MS Homo sapiens
7 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 Rab5b 19344
Affinity Capture-MS Mus musculus
9 HEXIM1 10614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BSPRY 54836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Nfya  
Affinity Capture-MS Mus musculus
12 SALL1 6299
Affinity Capture-MS Homo sapiens
13 C15orf59  
Affinity Capture-MS Homo sapiens
14 KIFAP3 22920
Affinity Capture-MS Homo sapiens
15 Aurkb  
Affinity Capture-MS Mus musculus
16 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 Cenpf  
Affinity Capture-MS Mus musculus
18 C9orf41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RILP 83547
Affinity Capture-MS Homo sapiens
20 NACC2  
Affinity Capture-MS Homo sapiens
21 ANKS3  
Affinity Capture-MS Homo sapiens
22 BIN3 55909
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NDC80 10403
Proximity Label-MS Homo sapiens
24 MEOX2  
Two-hybrid Homo sapiens
25 TSC22D2 9819
Two-hybrid Homo sapiens
26 BCL6  
Affinity Capture-MS Homo sapiens
27 CTNNA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 DMD 1756
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PRPF31 26121
Affinity Capture-MS Homo sapiens
30 Eea1  
Affinity Capture-MS Mus musculus
31 INO80E  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here