Gene description for ITGB2
Gene name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Gene symbol ITGB2
Other names/aliases CD18
LAD
LCAMB
LFA-1
MAC-1
MF17
MFI7
Species Homo sapiens
 Database cross references - ITGB2
ExoCarta ExoCarta_3689
Vesiclepedia VP_3689
Entrez Gene 3689
HGNC 6155
MIM 600065
UniProt P05107  
 ITGB2 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
T cells 11907077    
Thymus 23844026    
 Gene ontology annotations for ITGB2
Molecular Function
    amyloid-beta binding GO:0001540 IBA
    amyloid-beta binding GO:0001540 ISS
    complement component C3b binding GO:0001851 ISS
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 NAS
    integrin binding GO:0005178 TAS
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IPI
    ICAM-3 receptor activity GO:0030369 IMP
    heat shock protein binding GO:0031072 IPI
    cargo receptor activity GO:0038024 ISS
    metal ion binding GO:0046872 IEA
    cell adhesion molecule binding GO:0050839 IMP
Biological Process
    microglial cell activation GO:0001774 NAS
    leukocyte migration involved in inflammatory response GO:0002523 IEA
    receptor-mediated endocytosis GO:0006898 ISS
    phagocytosis, engulfment GO:0006911 ISS
    apoptotic process GO:0006915 NAS
    inflammatory response GO:0006954 NAS
    cell adhesion GO:0007155 IDA
    leukocyte cell-cell adhesion GO:0007159 IBA
    leukocyte cell-cell adhesion GO:0007159 IDA
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IDA
    cell-matrix adhesion GO:0007160 IMP
    cell-matrix adhesion GO:0007160 NAS
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 IMP
    integrin-mediated signaling pathway GO:0007229 NAS
    cell-cell signaling GO:0007267 NAS
    regulation of cell shape GO:0008360 NAS
    neutrophil chemotaxis GO:0030593 IBA
    neutrophil chemotaxis GO:0030593 IDA
    receptor internalization GO:0031623 ISS
    positive regulation of superoxide anion generation GO:0032930 IGI
    positive regulation of superoxide anion generation GO:0032930 NAS
    cell adhesion mediated by integrin GO:0033627 IBA
    heterotypic cell-cell adhesion GO:0034113 IMP
    endodermal cell differentiation GO:0035987 IEP
    receptor clustering GO:0043113 IMP
    positive regulation of neutrophil degranulation GO:0043315 IGI
    endothelial cell migration GO:0043542 IEA
    cellular extravasation GO:0045123 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    positive regulation of angiogenesis GO:0045766 IEA
    negative regulation of dopamine metabolic process GO:0045963 NAS
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IDA
    cellular response to low-density lipoprotein particle stimulus GO:0071404 ISS
    positive regulation of protein targeting to membrane GO:0090314 NAS
    amyloid-beta clearance GO:0097242 ISS
    cell-cell adhesion GO:0098609 ISS
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 NAS
    positive regulation of leukocyte adhesion to vascular endothelial cell GO:1904996 ISS
    neutrophil migration GO:1990266 IMP
    positive regulation of prostaglandin-E synthase activity GO:2000363 NAS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IBA
    integrin complex GO:0008305 IBA
    integrin complex GO:0008305 NAS
    external side of plasma membrane GO:0009897 NAS
    cell surface GO:0009986 HDA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    membrane GO:0016020 HDA
    integrin alphaL-beta2 complex GO:0034687 IDA
    integrin alphaL-beta2 complex GO:0034687 NAS
    integrin alphaM-beta2 complex GO:0034688 IPI
    integrin alphaM-beta2 complex GO:0034688 ISS
    integrin alphaX-beta2 complex GO:0034689 IPI
    integrin alphaX-beta2 complex GO:0034689 TAS
    specific granule membrane GO:0035579 TAS
    receptor complex GO:0043235 IDA
    plasma membrane raft GO:0044853 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
    extracellular vesicle GO:1903561 HDA
 Experiment description of studies that identified ITGB2 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 18
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors "Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C"
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITGB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EDEM2  
Affinity Capture-MS Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 RHBDD1 84236
Affinity Capture-MS Homo sapiens
4 SPTAN1 6709
Affinity Capture-Western Homo sapiens
5 MS4A7  
Two-hybrid Homo sapiens
6 WDR5 11091
Co-localization Homo sapiens
7 UPK1B 7348
Two-hybrid Homo sapiens
8 CD151 977
Affinity Capture-MS Homo sapiens
9 KMT2A  
Co-localization Homo sapiens
10 P2rx7  
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
11 PRKCD 5580
Biochemical Activity Homo sapiens
12 ICAM1 3383
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 YWHAB 7529
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 TM4SF18  
Two-hybrid Homo sapiens
15 MTIF3  
Two-hybrid Homo sapiens
16 KRTAP10-8  
Two-hybrid Homo sapiens
17 CD226 10666
Affinity Capture-Western Homo sapiens
18 PLAUR 5329
Affinity Capture-Western Homo sapiens
19 GNG8  
Affinity Capture-MS Homo sapiens
20 NBPF19  
Two-hybrid Homo sapiens
21 AMPD3 272
Affinity Capture-MS Homo sapiens
22 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
23 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
24 PSMD4 5710
Affinity Capture-Western Homo sapiens
25 CYTH1 9267
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 ITGAD  
Affinity Capture-Western Homo sapiens
27 DDX58 23586
Affinity Capture-RNA Homo sapiens
28 ITGAL 3683
Affinity Capture-Western Homo sapiens
29 SPTBN1 6711
Affinity Capture-Western Homo sapiens
30 SCARF2 91179
Affinity Capture-MS Homo sapiens
31 PRKCG 5582
Biochemical Activity Homo sapiens
32 ADAMTSL4  
Two-hybrid Homo sapiens
33 RANBP9 10048
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 CGRRF1  
Affinity Capture-MS Homo sapiens
35 APOL2 23780
Two-hybrid Homo sapiens
36 KLK10 5655
Affinity Capture-MS Homo sapiens
37 MYH9 4627
Affinity Capture-Western Homo sapiens
38 CYSRT1 375791
Two-hybrid Homo sapiens
39 PRKCA 5578
Biochemical Activity Homo sapiens
40 C3 718
Reconstituted Complex Homo sapiens
41 PTK2B 2185
Affinity Capture-Western Homo sapiens
42 KNG1 3827
Reconstituted Complex Homo sapiens
43 COPS5 10987
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
44 SHARPIN  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
45 VNN2  
Affinity Capture-Western Homo sapiens
46 GNB2L1 10399
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 SYK 6850
Affinity Capture-Western Homo sapiens
48 PRKCB 5579
Biochemical Activity Homo sapiens
49 LHFPL5  
Two-hybrid Homo sapiens
50 FHL2 2274
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 PRKCE 5581
Biochemical Activity Homo sapiens
52 APOA1 335
Cross-Linking-MS (XL-MS) Homo sapiens
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