Gene description for TOP2A
Gene name topoisomerase (DNA) II alpha 170kDa
Gene symbol TOP2A
Other names/aliases TOP2
TP2A
Species Homo sapiens
 Database cross references - TOP2A
ExoCarta ExoCarta_7153
Vesiclepedia VP_7153
Entrez Gene 7153
HGNC 11989
MIM 126430
UniProt P11388  
 TOP2A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Endothelial cells 26027894    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for TOP2A
Molecular Function
    magnesium ion binding GO:0000287 IDA
    DNA binding GO:0003677 IDA
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 HDA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IDA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IMP
    protein kinase C binding GO:0005080 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP-dependent activity, acting on DNA GO:0008094 IDA
    DNA binding, bending GO:0008301 IDA
    DNA negative supercoiling activity GO:0034335 IEA
    protein homodimerization activity GO:0042803 IPI
    ubiquitin binding GO:0043130 IMP
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    resolution of meiotic recombination intermediates GO:0000712 IBA
    sister chromatid segregation GO:0000819 IBA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    DNA topological change GO:0006265 IDA
    DNA topological change GO:0006265 IMP
    DNA ligation GO:0006266 IDA
    DNA damage response GO:0006974 IDA
    chromosome segregation GO:0007059 IMP
    female meiotic nuclear division GO:0007143 IEA
    apoptotic chromosome condensation GO:0030263 IBA
    apoptotic chromosome condensation GO:0030263 IDA
    embryonic cleavage GO:0040016 IEA
    regulation of circadian rhythm GO:0042752 ISS
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045870 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    rhythmic process GO:0048511 IEA
    negative regulation of DNA duplex unwinding GO:1905463 IMP
Subcellular Localization
    nuclear chromosome GO:0000228 IDA
    chromosome, centromeric region GO:0000775 IEA
    condensed chromosome GO:0000793 IEA
    male germ cell nucleus GO:0001673 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    centriole GO:0005814 IDA
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex GO:0009330 IDA
    protein-containing complex GO:0032991 IDA
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified TOP2A in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 1275
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 1280
MISEV standards
Biophysical techniques
✔ Enriched markers
✔ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 226
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
22
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 255
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 256
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 258
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
33
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
34
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
37
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
38
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
39
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
40
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TOP2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 HDAC2 3066
Affinity Capture-Western Homo sapiens
5 KPNA1 3836
Co-fractionation Homo sapiens
6 PRPF8 10594
Co-fractionation Homo sapiens
7 LLGL1 3996
Synthetic Lethality Homo sapiens
8 TOP2B 7155
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
9 FOXD3  
Affinity Capture-MS Homo sapiens
10 BRCA1 672
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 MAP4K4 9448
Affinity Capture-MS Homo sapiens
14 CSNK2A1 1457
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
15 VHL  
Affinity Capture-Western Homo sapiens
16 GCN1L1 10985
Co-fractionation Homo sapiens
17 PRKDC 5591
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 CENPA  
Proximity Label-MS Homo sapiens
19 DDX23 9416
Proximity Label-MS Homo sapiens
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 SF3B1 23451
Co-fractionation Homo sapiens
22 FBL 2091
Proximity Label-MS Homo sapiens
23 RPS9 6203
Affinity Capture-MS Homo sapiens
24 SNRPD3 6634
Co-fractionation Homo sapiens
25 RPS11 6205
Synthetic Lethality Homo sapiens
26 CKMT2  
Affinity Capture-MS Homo sapiens
27 KIF14 9928
Affinity Capture-MS Homo sapiens
28 NINL  
Proximity Label-MS Homo sapiens
29 RBMX 27316
Co-fractionation Homo sapiens
30 UNK  
Affinity Capture-RNA Homo sapiens
31 CHMP4C 92421
Affinity Capture-MS Homo sapiens
32 RNF4 6047
Affinity Capture-MS Homo sapiens
33 RBM4B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PRKCG 5582
Biochemical Activity Homo sapiens
35 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
36 Srp72  
Affinity Capture-MS Mus musculus
37 TTN 7273
Synthetic Lethality Homo sapiens
38 CKAP5 9793
Affinity Capture-MS Homo sapiens
39 RPL31 6160
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 BANF1 8815
Affinity Capture-MS Homo sapiens
41 MED25  
Affinity Capture-MS Homo sapiens
42 HECTD1 25831
Affinity Capture-MS Homo sapiens
43 SPANXN2  
Affinity Capture-MS Homo sapiens
44 SRPK3  
Affinity Capture-MS Homo sapiens
45 SRPK1 6732
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
46 PIPSL 266971
Affinity Capture-MS Homo sapiens
47 CD3EAP  
Proximity Label-MS Homo sapiens
48 ANLN 54443
Affinity Capture-MS Homo sapiens
49 RING1 6015
Affinity Capture-Western Homo sapiens
50 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
51 DNAJC21  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
52 C9orf78 51759
Affinity Capture-MS Homo sapiens
53 SIRT7  
Affinity Capture-MS Homo sapiens
54 POLD3  
Co-fractionation Homo sapiens
55 FKBP8 23770
Affinity Capture-MS Homo sapiens
56 EIF2S2 8894
Affinity Capture-MS Homo sapiens
57 RBBP4 5928
Affinity Capture-MS Homo sapiens
58 FN1 2335
Affinity Capture-MS Homo sapiens
59 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
60 C12orf65  
Proximity Label-MS Homo sapiens
61 PCBP1 5093
Proximity Label-MS Homo sapiens
62 KIF1BP 26128
Affinity Capture-MS Homo sapiens
63 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
64 DLST 1743
Affinity Capture-MS Homo sapiens
65 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
66 TCF7  
Affinity Capture-MS Homo sapiens
67 PSMC5 5705
Affinity Capture-MS Homo sapiens
68 HIST1H2AJ 8331
Affinity Capture-MS Homo sapiens
69 RPS16 6217
Affinity Capture-MS Homo sapiens
70 METTL14  
Affinity Capture-MS Homo sapiens
71 FTL 2512
Affinity Capture-MS Homo sapiens
72 ASB3 51130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 HIST1H2AC 8334
Affinity Capture-MS Homo sapiens
74 MTA1 9112
Affinity Capture-MS Homo sapiens
75 VAC14 55697
Affinity Capture-MS Homo sapiens
76 CDC37 11140
Affinity Capture-MS Homo sapiens
77 Atrx  
Affinity Capture-MS Mus musculus
78 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
79 CDC6  
Synthetic Lethality Homo sapiens
80 RUVBL2 10856
Affinity Capture-MS Homo sapiens
81 G3BP2 9908
Affinity Capture-MS Homo sapiens
82 MAGEA9  
Affinity Capture-MS Homo sapiens
83 RPS15A 6210
Affinity Capture-MS Homo sapiens
84 DYNC1H1 1778
Co-fractionation Homo sapiens
85 MCM5 4174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 RPS24 6229
Affinity Capture-MS Homo sapiens
87 ATG4B 23192
Affinity Capture-MS Homo sapiens
88 POLA1  
Co-fractionation Homo sapiens
89 PTGES3 10728
Affinity Capture-MS Homo sapiens
90 CUL7 9820
Affinity Capture-MS Homo sapiens
91 PSMC4 5704
Affinity Capture-MS Homo sapiens
92 POLD2 5425
Co-fractionation Homo sapiens
93 POLE3  
Co-fractionation Homo sapiens
94 PSMA6 5687
Affinity Capture-MS Homo sapiens
95 KPNA3 3839
Co-fractionation Homo sapiens
96 RPS17 6218
Affinity Capture-MS Homo sapiens
97 FMR1 2332
Affinity Capture-MS Homo sapiens
98 RPA2 6118
Affinity Capture-MS Homo sapiens
99 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
100 NOP56 10528
Affinity Capture-MS Homo sapiens
101 CHCHD1  
Proximity Label-MS Homo sapiens
102 RPL14 9045
Affinity Capture-MS Homo sapiens
103 ATM 472
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
104 SRSF2 6427
Co-fractionation Homo sapiens
105 RPS3 6188
Affinity Capture-MS Homo sapiens
106 RPS18 6222
Affinity Capture-MS Homo sapiens
107 NUCKS1 64710
Affinity Capture-MS Homo sapiens
108 PRC1 9055
Affinity Capture-MS Homo sapiens
109 NPM1 4869
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
110 MED27  
Affinity Capture-MS Homo sapiens
111 OBSL1 23363
Affinity Capture-MS Homo sapiens
112 HIST1H3A 8350
Proximity Label-MS Homo sapiens
113 NR4A1  
Affinity Capture-MS Homo sapiens
114 VCAM1 7412
Affinity Capture-MS Homo sapiens
115 PSMB3 5691
Affinity Capture-MS Homo sapiens
116 GSK3B 2932
Affinity Capture-Western Homo sapiens
117 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
118 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 DDX21 9188
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
120 RPL4 6124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 WHSC1 7468
Proximity Label-MS Homo sapiens
122 DRG1 4733
Affinity Capture-MS Homo sapiens
123 POLA2  
Co-fractionation Homo sapiens
124 TRIM24  
Affinity Capture-MS Homo sapiens
125 VRK1 7443
Affinity Capture-MS Homo sapiens
126 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
127 IWS1  
Affinity Capture-MS Homo sapiens
128 SNRPD1 6632
Co-fractionation Homo sapiens
129 RAD18  
Affinity Capture-MS Homo sapiens
130 EXOSC5 56915
Affinity Capture-MS Homo sapiens
131 PLK1 5347
Biochemical Activity Homo sapiens
132 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 PATL1 219988
Co-fractionation Homo sapiens
134 Rrbp1  
Affinity Capture-MS Mus musculus
135 MAGOH 4116
Affinity Capture-MS Homo sapiens
136 C7orf50 84310
Affinity Capture-MS Homo sapiens
137 HNRNPD 3184
Reconstituted Complex Homo sapiens
138 SMARCAD1  
Affinity Capture-MS Homo sapiens
139 RPS2 6187
Affinity Capture-MS Homo sapiens
140 CDK5R2 8941
Cross-Linking-MS (XL-MS) Homo sapiens
141 EIF6 3692
Affinity Capture-MS Homo sapiens
142 MEX3A  
Affinity Capture-RNA Homo sapiens
143 EGFR 1956
Negative Genetic Homo sapiens
144 WEE1 7465
Synthetic Lethality Homo sapiens
145 TFG 10342
Affinity Capture-MS Homo sapiens
146 MYC  
Affinity Capture-MS Homo sapiens
147 CSNK2A2 1459
Co-fractionation Homo sapiens
148 CSNK2B 1460
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
149 RCN2 5955
Cross-Linking-MS (XL-MS) Homo sapiens
150 DDRGK1 65992
Affinity Capture-MS Homo sapiens
151 BMI1  
Affinity Capture-Western Homo sapiens
152 LMNA 4000
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
153 EIF3H 8667
Affinity Capture-MS Homo sapiens
154 RPL19 6143
Affinity Capture-MS Homo sapiens
155 Top2b 21974
Affinity Capture-MS Mus musculus
156 RPL13 6137
Affinity Capture-MS Homo sapiens
157 MKRN1 23608
Affinity Capture-MS Homo sapiens
158 TERF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
159 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
160 ILF3 3609
Affinity Capture-MS Homo sapiens
161 H3F3A 3020
Affinity Capture-MS Homo sapiens
162 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Synthetic Growth Defect Homo sapiens
Affinity Capture-MS Homo sapiens
163 FEN1 2237
Affinity Capture-MS Homo sapiens
164 TRIM52  
Affinity Capture-MS Homo sapiens
165 SF3B4 10262
Co-fractionation Homo sapiens
166 RPS12 6206
Affinity Capture-MS Homo sapiens
167 RPS4X 6191
Affinity Capture-MS Homo sapiens
168 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
169 RPS5 6193
Affinity Capture-MS Homo sapiens
170 YTHDF3  
Affinity Capture-MS Homo sapiens
171 ESR1  
Affinity Capture-MS Homo sapiens
172 PRKCA 5578
Biochemical Activity Homo sapiens
173 GLYR1 84656
Co-fractionation Homo sapiens
174 RPS29 6235
Affinity Capture-MS Homo sapiens
175 NEK9 91754
Affinity Capture-MS Homo sapiens
176 RANBP2 5903
Co-fractionation Homo sapiens
177 INTU  
Affinity Capture-MS Homo sapiens
178 HSD3B2 3284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 HNRNPC 3183
Co-fractionation Homo sapiens
180 FAM131B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
181 GATAD2B 57459
Affinity Capture-MS Homo sapiens
182 TCEB1 6921
Affinity Capture-Western Homo sapiens
183 YWHAE 7531
Affinity Capture-Western Homo sapiens
184 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
185 FAU 2197
Affinity Capture-MS Homo sapiens
186 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
187 ATG13 9776
Affinity Capture-MS Homo sapiens
188 CAPZB 832
Affinity Capture-MS Homo sapiens
189 RPL10A 4736
Affinity Capture-MS Homo sapiens
190 LLPH  
Affinity Capture-MS Homo sapiens
191 EIF3B 8662
Affinity Capture-MS Homo sapiens
192 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
193 LSG1  
Affinity Capture-MS Homo sapiens
194 LINC00240  
Affinity Capture-MS Homo sapiens
195 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
196 ITGA4 3676
Affinity Capture-MS Homo sapiens
197 RPL29 6159
Cross-Linking-MS (XL-MS) Homo sapiens
198 ATG16L1 55054
Affinity Capture-MS Homo sapiens
199 PALB2  
Synthetic Lethality Homo sapiens
200 NCK1 4690
Affinity Capture-MS Homo sapiens
201 HSPA8 3312
Co-fractionation Homo sapiens
202 FOXP1 27086
Affinity Capture-MS Homo sapiens
203 CENPK  
Affinity Capture-MS Homo sapiens
204 RPL27 6155
Affinity Capture-MS Homo sapiens
205 RPL9 6133
Affinity Capture-MS Homo sapiens
206 RPS10 6204
Affinity Capture-MS Homo sapiens
207 BLM 641
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
208 NOP2 4839
Co-fractionation Homo sapiens
209 CBX3 11335
Proximity Label-MS Homo sapiens
210 MKRN2 23609
Affinity Capture-MS Homo sapiens
211 CHMP4B 128866
Affinity Capture-MS Homo sapiens
212 BTF3 689
Affinity Capture-MS Homo sapiens
213 DCP1A 55802
Affinity Capture-MS Homo sapiens
214 CDH1 999
Affinity Capture-MS Homo sapiens
215 RPL36 25873
Affinity Capture-MS Homo sapiens
216 RPA4  
Proximity Label-MS Homo sapiens
217 RPLP1 6176
Affinity Capture-MS Homo sapiens
218 DHX9 1660
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
219 C1orf115  
Affinity Capture-MS Homo sapiens
220 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
221 SEC61G 23480
Affinity Capture-MS Homo sapiens
222 NECAP1 25977
Affinity Capture-MS Homo sapiens
223 BZW1 9689
Co-fractionation Homo sapiens
224 HCLS1 3059
Cross-Linking-MS (XL-MS) Homo sapiens
225 RPL11 6135
Affinity Capture-MS Homo sapiens
226 ZRANB1 54764
Affinity Capture-MS Homo sapiens
227 SF3B2 10992
Co-fractionation Homo sapiens
228 ZBTB2 57621
Affinity Capture-MS Homo sapiens
229 CDC73  
Synthetic Lethality Homo sapiens
230 MSH2 4436
Synthetic Lethality Homo sapiens
231 BAZ1B 9031
Affinity Capture-MS Homo sapiens
232 ECT2 1894
Affinity Capture-MS Homo sapiens
233 FAM60A  
Affinity Capture-MS Homo sapiens
234 LMNB2 84823
Affinity Capture-MS Homo sapiens
235 RPL21 6144
Affinity Capture-MS Homo sapiens
236 PSMA2 5683
Affinity Capture-MS Homo sapiens
237 DHX30 22907
Affinity Capture-MS Homo sapiens
238 MDM4  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
239 RFC1 5981
Co-fractionation Homo sapiens
240 GIGYF2 26058
Affinity Capture-MS Homo sapiens
241 MED10  
Affinity Capture-MS Homo sapiens
242 PRPF19 27339
Co-fractionation Homo sapiens
243 FXR2 9513
Affinity Capture-MS Homo sapiens
244 RPS14 6208
Affinity Capture-MS Homo sapiens
245 TOP1 7150
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
246 RPL22 6146
Affinity Capture-MS Homo sapiens
247 HP1BP3 50809
Affinity Capture-MS Homo sapiens
248 RPL18 6141
Affinity Capture-MS Homo sapiens
249 RNF2  
Affinity Capture-MS Homo sapiens
250 KTN1 3895
Affinity Capture-MS Homo sapiens
251 Eif3e 16341
Affinity Capture-MS Mus musculus
252 CDH19  
Affinity Capture-MS Homo sapiens
253 XRCC5 7520
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
254 AHSA1 10598
Affinity Capture-Western Homo sapiens
255 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
256 CUL2 8453
Affinity Capture-Western Homo sapiens
257 RPL6 6128
Affinity Capture-MS Homo sapiens
258 RPS21 6227
Affinity Capture-MS Homo sapiens
259 PIN1 5300
Affinity Capture-Western Homo sapiens
260 PRRC2C 23215
Affinity Capture-MS Homo sapiens
261 RPA1 6117
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
262 SNRPA 6626
Co-fractionation Homo sapiens
263 PBRM1 55193
Affinity Capture-MS Homo sapiens
264 SETMAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
265 DFFB  
Reconstituted Complex Homo sapiens
266 LOC102724334 102724334
Cross-Linking-MS (XL-MS) Homo sapiens
267 MTA2 9219
Affinity Capture-MS Homo sapiens
268 PCNA 5111
Affinity Capture-MS Homo sapiens
269 PDZD8 118987
Affinity Capture-MS Homo sapiens
270 NF1 4763
Synthetic Lethality Homo sapiens
271 DUOX2 50506
Cross-Linking-MS (XL-MS) Homo sapiens
272 RPS7 6201
Affinity Capture-MS Homo sapiens
273 RPL8 6132
Affinity Capture-MS Homo sapiens
274 RPL28 6158
Affinity Capture-MS Homo sapiens
275 RPS27L 51065
Affinity Capture-MS Homo sapiens
276 SERBP1 26135
Affinity Capture-MS Homo sapiens
277 UHRF1 29128
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
278 RAC1 5879
Affinity Capture-MS Homo sapiens
279 PRKCB 5579
Biochemical Activity Homo sapiens
280 SSRP1 6749
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
281 UBA52 7311
Affinity Capture-MS Homo sapiens
282 CAPN2 824
Affinity Capture-MS Homo sapiens
283 UBE2H 7328
Affinity Capture-MS Homo sapiens
284 SLFN11 91607
Affinity Capture-MS Homo sapiens
285 PRIM2 5558
Co-fractionation Homo sapiens
286 RIT1 6016
Negative Genetic Homo sapiens
287 COIL  
Proximity Label-MS Homo sapiens
288 RPS3A 6189
Affinity Capture-MS Homo sapiens
289 RPL30 6156
Affinity Capture-MS Homo sapiens
290 RPL10 6134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
291 RPL15 6138
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
292 BASP1 10409
Cross-Linking-MS (XL-MS) Homo sapiens
293 SNRPD2 6633
Co-fractionation Homo sapiens
294 ILF2 3608
Co-fractionation Homo sapiens
295 USP36  
Affinity Capture-MS Homo sapiens
296 Spire2  
Affinity Capture-MS Mus musculus
297 SRP54 6729
Affinity Capture-MS Homo sapiens
298 CHD4 1108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
299 RPS20 6224
Affinity Capture-MS Homo sapiens
300 RPL24 6152
Affinity Capture-MS Homo sapiens
301 COPS5 10987
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
302 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
303 IMPDH2 3615
Affinity Capture-MS Homo sapiens
304 PRIM1  
Co-fractionation Homo sapiens
305 H2AFY2 55506
Affinity Capture-MS Homo sapiens
306 PRPS1 5631
Affinity Capture-MS Homo sapiens
307 HMGA1 3159
Affinity Capture-MS Homo sapiens
308 ABCE1 6059
Affinity Capture-MS Homo sapiens
309 ZNF451 26036
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
310 BRD4 23476
Affinity Capture-MS Homo sapiens
311 SRP68 6730
Affinity Capture-MS Homo sapiens
312 CDT1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
313 GATAD2A 54815
Affinity Capture-MS Homo sapiens
314 KLHL20  
Affinity Capture-MS Homo sapiens
315 Eif3i 54709
Affinity Capture-MS Mus musculus
316 PSMC6 5706
Affinity Capture-MS Homo sapiens
317 SSR3 6747
Affinity Capture-MS Homo sapiens
318 TNFRSF1B  
Affinity Capture-MS Homo sapiens
319 CTCF  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
320 PDS5B 23047
Cross-Linking-MS (XL-MS) Homo sapiens
321 MAPK1 5594
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
322 RB1 5925
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
323 E2F4  
Affinity Capture-MS Homo sapiens
324 H2AFZ 3015
Affinity Capture-MS Homo sapiens
325 CLOCK  
Affinity Capture-MS Homo sapiens
326 PPP1CA 5499
Co-fractionation Homo sapiens
327 POLD1 5424
Affinity Capture-MS Homo sapiens
328 COX15 1355
Affinity Capture-MS Homo sapiens
329 RPS28 6234
Affinity Capture-MS Homo sapiens
330 PIAS4  
Biochemical Activity Homo sapiens
331 PSMC1 5700
Affinity Capture-MS Homo sapiens
332 STAU1 6780
Affinity Capture-MS Homo sapiens
333 UBE2I 7329
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
334 BRCA2 675
Synthetic Lethality Homo sapiens
335 SMARCA4 6597
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
336 KMT2E  
Affinity Capture-MS Homo sapiens
337 CHEK1  
Synthetic Lethality Homo sapiens
338 SUMO2 6613
Reconstituted Complex Homo sapiens
339 FH 2271
Synthetic Lethality Homo sapiens
340 RPL27A 6157
Affinity Capture-MS Homo sapiens
341 PML 5371
Affinity Capture-MS Homo sapiens
342 POLE  
Co-fractionation Homo sapiens
343 CYLC1 1538
Cross-Linking-MS (XL-MS) Homo sapiens
344 H2AFY 9555
Affinity Capture-MS Homo sapiens
345 CEBPA  
Protein-peptide Homo sapiens
346 DAXX  
Affinity Capture-MS Homo sapiens
347 NUMA1 4926
Affinity Capture-MS Homo sapiens
348 RPLP2 6181
Affinity Capture-MS Homo sapiens
349 FANCD2  
Affinity Capture-MS Homo sapiens
350 EIF4G1 1981
Affinity Capture-MS Homo sapiens
351 GLE1 2733
Affinity Capture-MS Homo sapiens
352 RPL35 11224
Affinity Capture-MS Homo sapiens
353 PLRG1 5356
Co-fractionation Homo sapiens
354 CTNNB1 1499
Affinity Capture-MS Homo sapiens
355 SRSF1 6426
Co-fractionation Homo sapiens
356 RPL36A 6173
Affinity Capture-MS Homo sapiens
357 RPS6 6194
Proximity Label-MS Homo sapiens
358 RPL3 6122
Affinity Capture-MS Homo sapiens
359 RPL7 6129
Affinity Capture-MS Homo sapiens
360 AURKB 9212
Affinity Capture-MS Homo sapiens
361 JUN 3725
Far Western Homo sapiens
362 FKBP5 2289
Affinity Capture-MS Homo sapiens
363 L3MBTL3  
Affinity Capture-MS Homo sapiens
364 MYO6 4646
Proximity Label-MS Homo sapiens
365 MAP3K4 4216
Synthetic Lethality Homo sapiens
366 UPF3B 65109
Co-fractionation Homo sapiens
367 ARID1A 8289
Affinity Capture-Western Homo sapiens
368 VDAC1 7416
Affinity Capture-MS Homo sapiens
369 KRAS 3845
Negative Genetic Homo sapiens
370 BRD3 8019
Affinity Capture-MS Homo sapiens
371 EIF3M 10480
Affinity Capture-MS Homo sapiens
372 CCDC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
373 Cbx1  
Affinity Capture-MS Mus musculus
374 UBE3B 89910
Affinity Capture-MS Homo sapiens
375 GLUD1 2746
Affinity Capture-MS Homo sapiens
376 SIRT6  
Affinity Capture-MS Homo sapiens
377 SRRM1 10250
Co-fractionation Homo sapiens
378 RAD17  
Synthetic Lethality Homo sapiens
379 CUL5 8065
Affinity Capture-MS Homo sapiens
380 HMGN5 79366
Affinity Capture-MS Homo sapiens
381 SMURF1 57154
Affinity Capture-MS Homo sapiens
382 SRP72 6731
Affinity Capture-MS Homo sapiens
383 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
384 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
385 CWC15  
Co-fractionation Homo sapiens
386 NCAPH 23397
Affinity Capture-MS Homo sapiens
387 AIRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
388 RPL23 9349
Affinity Capture-MS Homo sapiens
389 RPS8 6202
Affinity Capture-MS Homo sapiens
390 XRCC6 2547
Affinity Capture-MS Homo sapiens
391 HNRNPU 3192
Affinity Capture-MS Homo sapiens
392 GNB2L1 10399
Affinity Capture-MS Homo sapiens
393 RPL17 6139
Affinity Capture-MS Homo sapiens
394 CDC5L 988
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here