Gene description for PRIM2
Gene name primase, DNA, polypeptide 2 (58kDa)
Gene symbol PRIM2
Other names/aliases PRIM2A
p58
Species Homo sapiens
 Database cross references - PRIM2
ExoCarta ExoCarta_5558
Vesiclepedia VP_5558
Entrez Gene 5558
HGNC 9370
MIM 176636
UniProt P49643  
 PRIM2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PRIM2
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IDA
    DNA/RNA hybrid binding GO:0071667 IDA
Biological Process
    DNA replication, synthesis of primer GO:0006269 IBA
    DNA replication, synthesis of primer GO:0006269 IDA
    DNA replication, synthesis of primer GO:0006269 ISS
    DNA replication initiation GO:0006270 IBA
    DNA replication initiation GO:0006270 IDA
    positive regulation of DNA primase activity GO:1903934 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    alpha DNA polymerase:primase complex GO:0005658 IBA
    alpha DNA polymerase:primase complex GO:0005658 IDA
    alpha DNA polymerase:primase complex GO:0005658 IPI
 Experiment description of studies that identified PRIM2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRIM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 KIF2A 3796
Proximity Label-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 TNFRSF1B  
Affinity Capture-MS Homo sapiens
5 BKRF1  
Affinity Capture-MS
6 TOP1 7150
Co-fractionation Homo sapiens
7 USP37  
Proximity Label-MS Homo sapiens
8 PRPS2 5634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CIAO1 9391
Affinity Capture-MS Homo sapiens
10 PSMD11 5717
Co-fractionation Homo sapiens
11 PSMC4 5704
Co-fractionation Homo sapiens
12 RNF31 55072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NLGN4Y 22829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 POLR3H  
Affinity Capture-MS Homo sapiens
15 ACTC1 70
Proximity Label-MS Homo sapiens
16 POLD2 5425
Co-fractionation Homo sapiens
17 GFOD1  
Affinity Capture-MS Homo sapiens
18 RPA1 6117
Co-fractionation Homo sapiens
19 AMMECR1  
Affinity Capture-MS Homo sapiens
20 EGFR 1956
Negative Genetic Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 PARK2  
Affinity Capture-MS Homo sapiens
23 OBFC1 79991
Affinity Capture-MS Homo sapiens
24 SUV39H1  
Two-hybrid Homo sapiens
25 MILR1  
Affinity Capture-MS Homo sapiens
26 BTF3L4 91408
Affinity Capture-MS Homo sapiens
27 PM20D2 135293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FAM96B 51647
Affinity Capture-MS Homo sapiens
29 RFC1 5981
Co-fractionation Homo sapiens
30 BAG6 7917
Affinity Capture-MS Homo sapiens
31 POLA2  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
32 MRPS18C  
Affinity Capture-MS Homo sapiens
33 POLD3  
Co-fractionation Homo sapiens
34 TOP2A 7153
Co-fractionation Homo sapiens
35 POLE3  
Co-fractionation Homo sapiens
36 DCAF10  
Affinity Capture-MS Homo sapiens
37 KDM1A 23028
Two-hybrid Homo sapiens
38 MMS19 64210
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 SHARPIN  
Affinity Capture-MS Homo sapiens
40 BTF3 689
Affinity Capture-MS Homo sapiens
41 BBS1 582
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 S100B 6285
Affinity Capture-MS Homo sapiens
44 RFXANK  
Affinity Capture-MS Homo sapiens
45 CDK20  
Affinity Capture-MS Homo sapiens
46 PNMA2  
Affinity Capture-MS Homo sapiens
47 LIG1 3978
Co-fractionation Homo sapiens
48 MOV10 4343
Affinity Capture-RNA Homo sapiens
49 POLA1  
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
50 DUSP9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RECK 8434
Two-hybrid Homo sapiens
52 PRIM1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
53 KRAS 3845
Negative Genetic Homo sapiens
54 PCNA 5111
Co-fractionation Homo sapiens
55 SLC31A1 1317
Affinity Capture-MS Homo sapiens
56 POLE  
Co-fractionation Homo sapiens
57 Mad2l1 56150
Affinity Capture-MS Mus musculus
58 SSRP1 6749
Affinity Capture-MS Homo sapiens
59 NEUROG3  
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 ATG7 10533
Affinity Capture-MS Homo sapiens
62 MAD2L1 4085
Affinity Capture-MS Homo sapiens
63 CDC37 11140
Co-fractionation Homo sapiens
64 ERP44 23071
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RAE1 8480
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PIPSL 266971
Affinity Capture-MS Homo sapiens
68 POLD1 5424
Co-fractionation Homo sapiens
69 OGT 8473
Reconstituted Complex Homo sapiens
70 PSMD13 5719
Co-fractionation Homo sapiens
View the network image/svg+xml



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