Gene description for RECK
Gene name reversion-inducing-cysteine-rich protein with kazal motifs
Gene symbol RECK
Other names/aliases ST15
Species Homo sapiens
 Database cross references - RECK
ExoCarta ExoCarta_8434
Vesiclepedia VP_8434
Entrez Gene 8434
HGNC 11345
MIM 605227
UniProt O95980  
 RECK identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RECK
Molecular Function
    endopeptidase inhibitor activity GO:0004866 IBA
    endopeptidase inhibitor activity GO:0004866 IDA
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    protein binding GO:0005515 IPI
    metalloendopeptidase inhibitor activity GO:0008191 IDA
    coreceptor activity GO:0015026 IEA
    Wnt-protein binding GO:0017147 IEA
Biological Process
    blood vessel maturation GO:0001955 IBA
    sprouting angiogenesis GO:0002040 IBA
    embryo implantation GO:0007566 IEA
    extracellular matrix organization GO:0030198 IBA
    negative regulation of cell migration GO:0030336 IMP
    embryonic forelimb morphogenesis GO:0035115 IEA
    regulation of angiogenesis GO:0045765 IEA
    canonical Wnt signaling pathway GO:0060070 IEA
    regulation of canonical Wnt signaling pathway GO:0060828 IBA
    regulation of establishment of blood-brain barrier GO:0090210 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    regulation of extracellular matrix organization GO:1903053 IMP
    negative regulation of metalloendopeptidase activity GO:1904684 IDA
    negative regulation of metalloendopeptidase activity GO:1904684 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    side of membrane GO:0098552 IEA
    Wnt signalosome GO:1990909 IEA
 Experiment description of studies that identified RECK in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RECK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TXN2 25828
Affinity Capture-MS Homo sapiens
2 PRIM2 5558
Two-hybrid Homo sapiens
3 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 DISC1 27185
Two-hybrid Homo sapiens
5 NR4A3  
Two-hybrid Homo sapiens
6 IFNA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 BMF  
Two-hybrid Homo sapiens
8 NUDT16L1 84309
Two-hybrid Homo sapiens
9 EFHC2  
Two-hybrid Homo sapiens
10 ZNF438  
Two-hybrid Homo sapiens
11 KRTAP10-8  
Two-hybrid Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 MCCC2 64087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MTNR1B  
Two-hybrid Homo sapiens
15 ADAM12 8038
Two-hybrid Homo sapiens
16 REL 5966
Two-hybrid Homo sapiens
17 TEKT4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 KEAP1 9817
Two-hybrid Homo sapiens
19 NBPF19  
Two-hybrid Homo sapiens
20 KRTAP3-1  
Two-hybrid Homo sapiens
21 NOV 4856
Two-hybrid Homo sapiens
22 SPSB2  
Two-hybrid Homo sapiens
23 KRTAP13-2  
Two-hybrid Homo sapiens
24 ZNF704  
Two-hybrid Homo sapiens
25 CEP44  
Two-hybrid Homo sapiens
26 CFP  
Two-hybrid Homo sapiens
27 MLPH  
Two-hybrid Homo sapiens
28 OXER1  
Two-hybrid Homo sapiens
29 TCF4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 KRTAP26-1  
Two-hybrid Homo sapiens
31 SSC4D  
Two-hybrid Homo sapiens
32 ACOX1 51
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CTIF  
Two-hybrid Homo sapiens
34 GLRX3 10539
Two-hybrid Homo sapiens
35 TRIM69 140691
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 MEOX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 CYSRT1 375791
Two-hybrid Homo sapiens
38 KRT40  
Two-hybrid Homo sapiens
39 LCN1 3933
Two-hybrid Homo sapiens
40 USHBP1  
Two-hybrid Homo sapiens
41 CTH 1491
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 TRIM39  
Two-hybrid Homo sapiens
43 INCA1  
Two-hybrid Homo sapiens
44 C22orf39  
Two-hybrid Homo sapiens
View the network image/svg+xml



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