Gene description for MCCC2
Gene name methylcrotonoyl-CoA carboxylase 2 (beta)
Gene symbol MCCC2
Other names/aliases MCCB
Species Homo sapiens
 Database cross references - MCCC2
ExoCarta ExoCarta_64087
Vesiclepedia VP_64087
Entrez Gene 64087
HGNC 6937
MIM 609014
UniProt Q9HCC0  
 MCCC2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for MCCC2
Molecular Function
    methylcrotonoyl-CoA carboxylase activity GO:0004485 IBA
    methylcrotonoyl-CoA carboxylase activity GO:0004485 IDA
    methylcrotonoyl-CoA carboxylase activity GO:0004485 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    L-leucine catabolic process GO:0006552 IBA
    L-leucine catabolic process GO:0006552 IEA
    L-leucine catabolic process GO:0006552 TAS
    branched-chain amino acid catabolic process GO:0009083 NAS
    coenzyme A metabolic process GO:0015936 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 NAS
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 TAS
    methylcrotonoyl-CoA carboxylase complex GO:1905202 IBA
    methylcrotonoyl-CoA carboxylase complex GO:1905202 IDA
    methylcrotonoyl-CoA carboxylase complex GO:1905202 IPI
    methylcrotonoyl-CoA carboxylase complex GO:1905202 NAS
    methylcrotonoyl-CoA carboxylase complex GO:1905202 TAS
 Experiment description of studies that identified MCCC2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
3 DNAJC4  
Affinity Capture-MS Homo sapiens
4 P4HB 5034
Co-fractionation Homo sapiens
5 NDUFB11 54539
Co-fractionation Homo sapiens
6 ECH1 1891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PER2  
Proximity Label-MS Homo sapiens
9 HOOK1  
Proximity Label-MS Homo sapiens
10 CYB5B 80777
Affinity Capture-MS Homo sapiens
11 TERF2  
Affinity Capture-MS Homo sapiens
12 HSPB2  
Two-hybrid Homo sapiens
13 SLFN11 91607
Proximity Label-MS Homo sapiens
14 NR4A1  
Affinity Capture-MS Homo sapiens
15 NDUFB10 4716
Co-fractionation Homo sapiens
16 NDUFS1 4719
Co-fractionation Homo sapiens
17 MCCC2 64087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HSPA8 3312
Affinity Capture-MS Homo sapiens
19 C9orf78 51759
Affinity Capture-MS Homo sapiens
20 ICT1 3396
Affinity Capture-MS Homo sapiens
21 TPP1 1200
Co-fractionation Homo sapiens
22 HMGCL 3155
Co-fractionation Homo sapiens
23 RPA2 6118
Proximity Label-MS Homo sapiens
24 EIF2AK2 5610
Co-fractionation Homo sapiens
25 ADRB2  
Affinity Capture-MS Homo sapiens
26 LGALS3 3958
Affinity Capture-MS Homo sapiens
27 EBNA-LP  
Affinity Capture-MS
28 FBXL19  
Affinity Capture-MS Homo sapiens
29 PDHA1 5160
Proximity Label-MS Homo sapiens
30 EPS15 2060
Affinity Capture-MS Homo sapiens
31 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
33 MCCC1 56922
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
34 INPPL1 3636
Affinity Capture-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 OTUB1 55611
Affinity Capture-MS Homo sapiens
37 PREP 5550
Co-fractionation Homo sapiens
38 MTHFD1 4522
Co-fractionation Homo sapiens
39 TXN 7295
Co-fractionation Homo sapiens
40 ARRDC3 57561
Affinity Capture-MS Homo sapiens
41 PMPCB 9512
Affinity Capture-MS Homo sapiens
42 NDUFB9 4715
Co-fractionation Homo sapiens
43 DYNC1H1 1778
Co-fractionation Homo sapiens
44 WWP2 11060
Affinity Capture-MS Homo sapiens
45 ACACA 31
Affinity Capture-MS Homo sapiens
46 PAF1 54623
Affinity Capture-MS Homo sapiens
47 TERF1 7013
Affinity Capture-MS Homo sapiens
48 UBASH3B 84959
Affinity Capture-MS Homo sapiens
49 NDUFS8 4728
Co-fractionation Homo sapiens
50 CORO1B 57175
Co-fractionation Homo sapiens
51 IFIT1 3434
Affinity Capture-MS Homo sapiens
52 RPA3 6119
Proximity Label-MS Homo sapiens
53 PC 5091
Affinity Capture-MS Homo sapiens
54 ATP5E 514
Affinity Capture-MS Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 RXRA 6256
Co-fractionation Homo sapiens
57 BICD1 636
Proximity Label-MS Homo sapiens
58 PCCB 5096
Affinity Capture-MS Homo sapiens
59 SPRTN  
Affinity Capture-MS Homo sapiens
60 EP300 2033
Affinity Capture-MS Homo sapiens
61 C1QBP 708
Affinity Capture-MS Homo sapiens
62 NAP1L5  
Affinity Capture-MS Homo sapiens
63 RECK 8434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 MRPS24 64951
Affinity Capture-MS Homo sapiens
65 NDUFA5 4698
Co-fractionation Homo sapiens
66 VSX1  
Proximity Label-MS Homo sapiens
67 GCAT  
Affinity Capture-MS Homo sapiens
68 NAGA 4668
Co-fractionation Homo sapiens
69 POU5F1  
Affinity Capture-MS Homo sapiens
70 ATP5C1 509
Affinity Capture-MS Homo sapiens
71 PCCA 5095
Affinity Capture-MS Homo sapiens
72 HSPD1 3329
Co-fractionation Homo sapiens
73 NANOG  
Affinity Capture-MS Homo sapiens
74 CARS 833
Co-fractionation Homo sapiens
75 C9orf72  
Affinity Capture-MS Homo sapiens
76 PMPCA 23203
Affinity Capture-MS Homo sapiens
77 ATP5B 506
Co-fractionation Homo sapiens
78 HUWE1 10075
Affinity Capture-MS Homo sapiens
79 ECSIT 51295
Affinity Capture-MS Homo sapiens
80 NDUFS7 374291
Affinity Capture-MS Homo sapiens
81 OGT 8473
Reconstituted Complex Homo sapiens
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