Gene description for EIF2AK2
Gene name eukaryotic translation initiation factor 2-alpha kinase 2
Gene symbol EIF2AK2
Other names/aliases EIF2AK1
PKR
PPP1R83
PRKR
Species Homo sapiens
 Database cross references - EIF2AK2
ExoCarta ExoCarta_5610
Vesiclepedia VP_5610
Entrez Gene 5610
HGNC 9437
MIM 176871
UniProt P19525  
 EIF2AK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Thymus 23844026    
 Gene ontology annotations for EIF2AK2
Molecular Function
    RNA binding GO:0003723 HDA
    double-stranded RNA binding GO:0003725 IDA
    protein kinase activity GO:0004672 IDA
    protein kinase activity GO:0004672 IMP
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IBA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IMP
    ribosomal protein S6 kinase activity GO:0004711 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 IDA
    protein phosphatase regulator activity GO:0019888 TAS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    positive regulation of cytokine production GO:0001819 ISS
    chromatin remodeling GO:0006338 IEA
    translation GO:0006412 IEA
    regulation of translational initiation GO:0006446 IBA
    protein phosphorylation GO:0006468 IDA
    negative regulation of cell population proliferation GO:0008285 TAS
    response to virus GO:0009615 IMP
    negative regulation of translation GO:0017148 IBA
    negative regulation of translation GO:0017148 IDA
    negative regulation of translation GO:0017148 IMP
    endoplasmic reticulum unfolded protein response GO:0030968 IEA
    positive regulation of chemokine production GO:0032722 ISS
    positive regulation of stress-activated MAPK cascade GO:0032874 ISS
    negative regulation of osteoblast proliferation GO:0033689 IMP
    cellular response to amino acid starvation GO:0034198 IMP
    response to interferon-alpha GO:0035455 IDA
    negative regulation of apoptotic process GO:0043066 IEA
    positive regulation of MAPK cascade GO:0043410 IMP
    negative regulation of viral genome replication GO:0045071 IMP
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    defense response to virus GO:0051607 IEP
    antiviral innate immune response GO:0140374 IDA
    regulation of NLRP3 inflammasome complex assembly GO:1900225 ISS
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISS
    regulation of hematopoietic progenitor cell differentiation GO:1901532 ISS
    regulation of hematopoietic stem cell proliferation GO:1902033 ISS
    regulation of hematopoietic stem cell differentiation GO:1902036 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 TAS
    membrane GO:0016020 HDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified EIF2AK2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
14
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2AK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 HNRNPC 3183
Affinity Capture-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MACROD1 28992
Affinity Capture-MS Homo sapiens
6 COIL  
Proximity Label-MS Homo sapiens
7 ZNF346  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
8 FBXO32  
Affinity Capture-MS Homo sapiens
9 FNDC8  
Affinity Capture-MS Homo sapiens
10 BTF3 689
Affinity Capture-MS Homo sapiens
11 ARFGAP2 84364
Co-fractionation Homo sapiens
12 TRUB2  
Proximity Label-MS Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 CMPK1 51727
Co-fractionation Homo sapiens
15 RPL10 6134
Affinity Capture-MS Homo sapiens
16 CHCHD1  
Proximity Label-MS Homo sapiens
17 SSB 6741
Affinity Capture-MS Homo sapiens
18 XRN2 22803
Affinity Capture-MS Homo sapiens
19 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
20 CAND1 55832
Affinity Capture-MS Homo sapiens
21 RPUSD4 84881
Proximity Label-MS Homo sapiens
22 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
23 CCDC124 115098
Affinity Capture-MS Homo sapiens
24 MCCC2 64087
Co-fractionation Homo sapiens
25 SENP3 26168
Affinity Capture-MS Homo sapiens
26 MTRF1  
Proximity Label-MS Homo sapiens
27 ILF2 3608
Biochemical Activity Homo sapiens
28 PPP1CC 5501
Affinity Capture-MS Homo sapiens
29 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 FBXW7  
Affinity Capture-MS Homo sapiens
31 PDGFRB 5159
Affinity Capture-Western Homo sapiens
32 MCM2 4171
Affinity Capture-MS Homo sapiens
33 TRIM35 23087
Affinity Capture-MS Homo sapiens
34 RFXANK  
Affinity Capture-MS Homo sapiens
35 PRKY  
Affinity Capture-MS Homo sapiens
36 METAP2 10988
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 NPM1 4869
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
38 ANOS1 3730
Affinity Capture-MS Homo sapiens
39 OBSL1 23363
Affinity Capture-MS Homo sapiens
40 HIST1H2AB 8335
Biochemical Activity Homo sapiens
41 STAT3 6774
Affinity Capture-Western Homo sapiens
42 CDK3 1018
Affinity Capture-MS Homo sapiens
43 STAU2 27067
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
44 PRKRA 8575
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 HIST2H2AC 8338
Biochemical Activity Homo sapiens
46 GNL3 26354
Affinity Capture-MS Homo sapiens
47 RRP8  
Affinity Capture-MS Homo sapiens
48 RPS3A 6189
Affinity Capture-MS Homo sapiens
49 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
51 MYCN  
Affinity Capture-MS Homo sapiens
52 FASTKD2  
Proximity Label-MS Homo sapiens
53 Kif18b  
Affinity Capture-MS Mus musculus
54 PABPC4 8761
Affinity Capture-MS Homo sapiens
55 RPL19 6143
Affinity Capture-MS Homo sapiens
56 MTERF3  
Proximity Label-MS Homo sapiens
57 NEIL3  
Protein-RNA Homo sapiens
58 IFNA14  
Affinity Capture-MS Homo sapiens
59 FAS 355
Proximity Label-MS Homo sapiens
60 DUS2 54920
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
61 EDC4 23644
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
62 FARSA 2193
Affinity Capture-MS Homo sapiens
63 EIF2AK2 5610
Two-hybrid Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
64 PPP1CA 5499
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 FAM96A  
Affinity Capture-MS Homo sapiens
66 DLD 1738
Affinity Capture-MS Homo sapiens
67 DBNL 28988
Affinity Capture-MS Homo sapiens
68 COX15 1355
Affinity Capture-MS Homo sapiens
69 PARK2  
Affinity Capture-MS Homo sapiens
70 ZC3H3  
Affinity Capture-MS Homo sapiens
71 TARBP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
72 HMGN2 3151
Co-fractionation Homo sapiens
73 STAU1 6780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 NOP2 4839
Affinity Capture-MS Homo sapiens
75 GCH1  
Affinity Capture-MS Homo sapiens
76 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
77 Jak2 16452
Biochemical Activity Mus musculus
78 CHMP4B 128866
Affinity Capture-MS Homo sapiens
79 TYK2 7297
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
80 TRIM21 6737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
81 NTRK1 4914
Affinity Capture-MS Homo sapiens
82 DHX9 1660
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
83 IFRD1 3475
Affinity Capture-MS Homo sapiens
84 IFI16 3428
Affinity Capture-MS Homo sapiens
85 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
86 MOV10 4343
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
87 DICER1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
88 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
89 LYAR 55646
Affinity Capture-MS Homo sapiens
90 Rrbp1  
Affinity Capture-MS Mus musculus
91 HECTD1 25831
Affinity Capture-MS Homo sapiens
92 RPLP0 6175
Affinity Capture-MS Homo sapiens
93 GTPBP4 23560
Affinity Capture-MS Homo sapiens
94 GLTSCR2  
Affinity Capture-MS Homo sapiens
95 UBXN6 80700
Affinity Capture-MS Homo sapiens
96 USP32 84669
Affinity Capture-MS Homo sapiens
97 PSG11  
Affinity Capture-MS Homo sapiens
98 OGT 8473
Reconstituted Complex Homo sapiens
99 CCNA2 890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 NOC3L 64318
Affinity Capture-MS Homo sapiens
101 TOP1 7150
Affinity Capture-MS Homo sapiens
102 RPS2 6187
Affinity Capture-MS Homo sapiens
103 EIF6 3692
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
104 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
105 CUL3 8452
Affinity Capture-MS Homo sapiens
106 PTEN 5728
Affinity Capture-Western Homo sapiens
107 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 DHX30 22907
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
109 IQCF1  
Affinity Capture-MS Homo sapiens
110 MAP3K7  
Affinity Capture-Western Homo sapiens
111 ZC3H11A  
Affinity Capture-MS Homo sapiens
112 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
113 ELMSAN1  
Affinity Capture-MS Homo sapiens
114 C12orf65  
Proximity Label-MS Homo sapiens
115 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
116 FARSB 10056
Affinity Capture-MS Homo sapiens
117 PCBP1 5093
Affinity Capture-MS Homo sapiens
118 IL7R  
Protein-RNA Homo sapiens
119 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
120 MYC  
Affinity Capture-MS Homo sapiens
121 HECW2  
Affinity Capture-MS Homo sapiens
122 JAK1 3716
Affinity Capture-Western Homo sapiens
123 EGFR 1956
Affinity Capture-MS Homo sapiens
124 DLST 1743
Affinity Capture-MS Homo sapiens
125 TRAF5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
126 RPL35A 6165
Affinity Capture-MS Homo sapiens
127 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
128 DNAJC3 5611
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
129 RPL18 6141
Reconstituted Complex Homo sapiens
130 DDRGK1 65992
Affinity Capture-MS Homo sapiens
131 G3BP1 10146
Reconstituted Complex Homo sapiens
132 PPP2R5A 5525
Affinity Capture-Western Homo sapiens
133 LMNA 4000
Affinity Capture-MS Homo sapiens
134 ADAR 103
Affinity Capture-MS Homo sapiens
135 CHUK 1147
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
136 FTSJ3 117246
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
137 HDGF 3068
Affinity Capture-MS Homo sapiens
138 METTL21B  
Affinity Capture-MS Homo sapiens
139 RPS16 6217
Affinity Capture-MS Homo sapiens
140 EIF2S1 1965
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
141 SURF6  
Affinity Capture-MS Homo sapiens
142 MRPL42  
Affinity Capture-MS Homo sapiens
143 SH3PXD2B 285590
Co-fractionation Homo sapiens
144 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
145 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
146 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
147 ILF3 3609
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
148 CKAP4 10970
Affinity Capture-MS Homo sapiens
149 FANCC 2176
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
150 RPL6 6128
Affinity Capture-MS Homo sapiens
151 ICT1 3396
Proximity Label-MS Homo sapiens
152 MEN1 4221
Affinity Capture-MS Homo sapiens
153 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
154 IKBKB 3551
Affinity Capture-Western Homo sapiens
155 NHLRC2 374354
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
156 SEPHS2  
Co-fractionation Homo sapiens
157 PRKRIP1 79706
Affinity Capture-Western Homo sapiens
158 CAPRIN1 4076
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
159 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
160 DDX58 23586
Affinity Capture-Western Homo sapiens
161 ATP6V1F 9296
Co-fractionation Homo sapiens
162 UFL1 23376
Affinity Capture-MS Homo sapiens
163 DHX58 79132
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
164 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
165 TBRG4 9238
Proximity Label-MS Homo sapiens
166 TRIP4 9325
Affinity Capture-MS Homo sapiens
167 TRIM31  
Affinity Capture-MS Homo sapiens
168 CIT 11113
Affinity Capture-MS Homo sapiens
169 TOLLIP 54472
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
170 IGF2BP2 10644
Affinity Capture-MS Homo sapiens
171 GRSF1 2926
Proximity Label-MS Homo sapiens
172 KCTD17 79734
Affinity Capture-MS Homo sapiens
173 PTGES3 10728
Affinity Capture-Western Homo sapiens
174 CARS 833
Co-fractionation Homo sapiens
175 GPX7 2882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
176 PURA 5813
Affinity Capture-MS Homo sapiens
177 CCT8L2  
Affinity Capture-MS Homo sapiens
178 PRMT1 3276
Affinity Capture-MS Homo sapiens
179 SCARB2 950
Affinity Capture-MS Homo sapiens
180 PTGR2 145482
Co-fractionation Homo sapiens
181 PRKRIR  
Affinity Capture-Western Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here