Gene description for ATP6V1F
Gene name ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
Gene symbol ATP6V1F
Other names/aliases ATP6S14
VATF
Vma7
Species Homo sapiens
 Database cross references - ATP6V1F
ExoCarta ExoCarta_9296
Vesiclepedia VP_9296
Entrez Gene 9296
HGNC 16832
MIM 607160
UniProt Q16864  
 ATP6V1F identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for ATP6V1F
Molecular Function
    protein binding GO:0005515 IPI
    proton transmembrane transporter activity GO:0015078 NAS
    ATPase-coupled ion transmembrane transporter activity GO:0042625 NAS
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
Biological Process
    vacuolar acidification GO:0007035 NAS
    lysosomal lumen acidification GO:0007042 NAS
    endosomal lumen acidification GO:0048388 NAS
    intracellular pH reduction GO:0051452 NAS
    Golgi lumen acidification GO:0061795 NAS
    synaptic vesicle lumen acidification GO:0097401 IEA
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 ISS
    lysosomal membrane GO:0005765 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 NAS
    endosome membrane GO:0010008 NAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    synaptic vesicle membrane GO:0030672 IEA
    proton-transporting V-type ATPase complex GO:0033176 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ATP6V1F in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP6V1F
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 IPO7 10527
Affinity Capture-MS Homo sapiens
5 KIAA2013 90231
Affinity Capture-MS Homo sapiens
6 ZPR1 8882
Co-fractionation Homo sapiens
7 USP9X 8239
Co-fractionation Homo sapiens
8 DNAJC25 548645
Proximity Label-MS Homo sapiens
9 MID1 4281
Affinity Capture-MS Homo sapiens
10 PTPN1 5770
Proximity Label-MS Homo sapiens
11 SRSF2 6427
Co-fractionation Homo sapiens
12 VASP 7408
Co-fractionation Homo sapiens
13 VPS26A 9559
Co-fractionation Homo sapiens
14 CORO1B 57175
Co-fractionation Homo sapiens
15 WDR7 23335
Affinity Capture-MS Homo sapiens
16 CCDC115 84317
Affinity Capture-MS Homo sapiens
17 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ATP6V0A4 50617
Affinity Capture-MS Homo sapiens
19 ATP6AP1 537
Affinity Capture-MS Homo sapiens
20 UBE2V1 7335
Co-fractionation Homo sapiens
21 UNK  
Co-fractionation Homo sapiens
22 NNT 23530
Co-fractionation Homo sapiens
23 ATP6V1G1 9550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 EIF2AK2 5610
Co-fractionation Homo sapiens
25 MPV17 4358
Co-fractionation Homo sapiens
26 UBE4B 10277
Co-fractionation Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 ATP6V1G2  
Affinity Capture-MS Homo sapiens
29 UBXN7 26043
Co-fractionation Homo sapiens
30 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
31 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
32 UBR5 51366
Co-fractionation Homo sapiens
33 PLXDC2 84898
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 VMA21  
Affinity Capture-MS Homo sapiens
37 RHOT2 89941
Proximity Label-MS Homo sapiens
38 UBE2D1 7321
Co-fractionation Homo sapiens
39 VDAC2 7417
Co-fractionation Homo sapiens
40 ATP6V1E1 529
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TTLL12 23170
Co-fractionation Homo sapiens
42 TMEM185A  
Affinity Capture-MS Homo sapiens
43 IGBP1 3476
Co-fractionation Homo sapiens
44 ATP6V1D 51382
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ROGDI 79641
Affinity Capture-MS Homo sapiens
46 SEC63 11231
Proximity Label-MS Homo sapiens
47 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
48 UFC1 51506
Co-fractionation Homo sapiens
49 SRXN1 140809
Co-fractionation Homo sapiens
50 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 LAMP1 3916
Proximity Label-MS Homo sapiens
52 GOLT1B 51026
Affinity Capture-MS Homo sapiens
53 Rmdn3  
Affinity Capture-MS Mus musculus
54 TMEM199  
Affinity Capture-MS Homo sapiens
55 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DMXL1 1657
Affinity Capture-MS Homo sapiens
57 FKBP8 23770
Proximity Label-MS Homo sapiens
58 KIAA1377  
Two-hybrid Homo sapiens
59 CORO1C 23603
Co-fractionation Homo sapiens
60 Vps4b 20479
Affinity Capture-MS Mus musculus
61 TCIRG1 10312
Affinity Capture-MS Homo sapiens
62 ATP6V1A 523
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ABCC1 4363
Co-fractionation Homo sapiens
64 DDB1 1642
Co-fractionation Homo sapiens
65 HYOU1 10525
Co-fractionation Homo sapiens
66 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 C9orf16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SLC14A1  
Affinity Capture-MS Homo sapiens
69 AIMP1 9255
Co-fractionation Homo sapiens
70 ATP6V1H 51606
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
71 DMXL2  
Affinity Capture-MS Homo sapiens
72 ZRANB2 9406
Co-fractionation Homo sapiens
73 ARRDC5  
Affinity Capture-MS Homo sapiens
74 DDX3X 1654
Co-fractionation Homo sapiens
75 UBE2C 11065
Co-fractionation Homo sapiens
76 PICALM 8301
Co-fractionation Homo sapiens
77 UCHL3 7347
Co-fractionation Homo sapiens
78 TXN 7295
Co-fractionation Homo sapiens
79 ATP6V0C 527
Affinity Capture-MS Homo sapiens
80 MOV10 4343
Affinity Capture-RNA Homo sapiens
81 HNRNPL 3191
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
82 UBE2B 7320
Co-fractionation Homo sapiens
83 RAB7A 7879
Proximity Label-MS Homo sapiens
84 SSSCA1 10534
Co-fractionation Homo sapiens
85 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 KRAS 3845
Negative Genetic Homo sapiens
87 TLDC2  
Affinity Capture-MS Homo sapiens
88 NEDD8 4738
Co-fractionation Homo sapiens
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