Experiment description of studies that identified UFC1 in sEVs

1 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

8 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for UFC1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Co-fractionation Homo sapiens 2 ATP6V0D1 9114 Cross-Linking-MS (XL-MS) Homo sapiens 3 RPA2 6118 Proximity Label-MS Homo sapiens 4 RIT1 6016 Negative Genetic Homo sapiens 5 CAT 847 Co-fractionation Homo sapiens 6 COX17   Co-fractionation Homo sapiens 7 FASN 2194 Negative Genetic Homo sapiens 8 ZPR1 8882 Co-fractionation Homo sapiens 9 NEDD8 4738 Co-fractionation Homo sapiens 10 ETFA 2108 Co-fractionation Homo sapiens 11 SOD1 6647 Co-fractionation Homo sapiens 12 USP34 9736 Co-fractionation Homo sapiens 13 SAR1B 51128 Affinity Capture-MS Homo sapiens 14 AHCYL1 10768 Co-fractionation Homo sapiens 15 ATP6V1B2 526 Co-fractionation Homo sapiens 16 LDHB 3945 Co-fractionation Homo sapiens 17 TYMS 7298 Co-fractionation Homo sapiens 18 PCMT1 5110 Co-fractionation Homo sapiens 19 UNK   Co-fractionation Homo sapiens 20 UBXN7 26043 Co-fractionation Homo sapiens 21 ZYX 7791 Co-fractionation Homo sapiens 22 CRIP1 1396 Co-fractionation Homo sapiens 23 UBA52 7311 Co-fractionation Homo sapiens 24 USP9X 8239 Co-fractionation Homo sapiens 25 NECAP1 25977 Co-fractionation Homo sapiens 26 LYPLA2 11313 Co-fractionation Homo sapiens 27 TKT 7086 Co-fractionation Homo sapiens 28 FKBP1A 2280 Co-fractionation Homo sapiens 29 GEMIN4 50628 Two-hybrid Homo sapiens 30 CLNS1A 1207 Co-fractionation Homo sapiens 31 ATP6V1F 9296 Co-fractionation Homo sapiens 32 UFL1 23376 Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 TALDO1 6888 Co-fractionation Homo sapiens 34 RPA3 6119 Proximity Label-MS Homo sapiens 35 NECAP2 55707 Co-fractionation Homo sapiens 36 CAPG 822 Co-fractionation Homo sapiens 37 TAGLN2 8407 Co-fractionation Homo sapiens 38 UBA3 9039 Co-fractionation Homo sapiens 39 AURKA 6790 Affinity Capture-MS Homo sapiens 40 WDYHV1   Two-hybrid Homo sapiens 41 C16orf72 29035 Affinity Capture-MS Homo sapiens 42 GAPVD1 26130 Cross-Linking-MS (XL-MS) Homo sapiens 43 EGFR 1956 Negative Genetic Homo sapiens 44 SPR 6697 Co-fractionation Homo sapiens 45 SSSCA1 10534 Co-fractionation Homo sapiens 46 YKT6 10652 Co-fractionation Homo sapiens 47 CDK5RAP3 80279 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 ANKRD11 29123 Two-hybrid Homo sapiens 49 UBA5 79876 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 TTC9C 283237 Co-fractionation Homo sapiens 51 DDRGK1 65992 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 Ufl1   Affinity Capture-MS Mus musculus 53 KRAS 3845 Negative Genetic Homo sapiens 54 VCL 7414 Co-fractionation Homo sapiens 55 PSMC2 5701 Co-fractionation Homo sapiens 56 CCR1   Affinity Capture-MS Homo sapiens 57 HINT1 3094 Co-fractionation Homo sapiens 58 ZFYVE19 84936 Co-fractionation Homo sapiens 59 UBXN1 51035 Co-fractionation Homo sapiens 60 UFM1 51569 Affinity Capture-Western Homo sapiens Co-fractionation Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 61 HUWE1 10075 Affinity Capture-MS Homo sapiens 62 PIPSL 266971 Co-fractionation Homo sapiens View the network

Pathways in which UFC1 is involved

No pathways found