Gene description for PPP2R5A
Gene name protein phosphatase 2, regulatory subunit B', alpha
Gene symbol PPP2R5A
Other names/aliases B56A
PR61A
Species Homo sapiens
 Database cross references - PPP2R5A
ExoCarta ExoCarta_5525
Vesiclepedia VP_5525
Entrez Gene 5525
HGNC 9309
MIM 601643
UniProt Q15172  
 PPP2R5A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPP2R5A
Molecular Function
    protein binding GO:0005515 IPI
    kinase inhibitor activity GO:0019210 IMP
    protein phosphatase regulator activity GO:0019888 IDA
    kinase binding GO:0019900 IPI
    protein phosphatase activator activity GO:0072542 IBA
    protein phosphatase activator activity GO:0072542 IMP
Biological Process
    signal transduction GO:0007165 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IBA
    protein phosphatase type 2A complex GO:0000159 IDA
    protein phosphatase type 2A complex GO:0000159 IMP
    chromosome, centromeric region GO:0000775 IEA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    Z disc GO:0030018 IDA
    M band GO:0031430 ISS
 Experiment description of studies that identified PPP2R5A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPP2R5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ESPL1  
Co-purification Homo sapiens
2 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
3 KNDC1  
Affinity Capture-MS Homo sapiens
4 RNF41  
Affinity Capture-MS Homo sapiens
5 UBE2S  
Affinity Capture-Western Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 Sgol2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
8 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
9 PPP2R5E 5529
Affinity Capture-MS Homo sapiens
10 CDC20 991
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 SLFN11 91607
Affinity Capture-MS Homo sapiens
12 FAM13A 10144
Affinity Capture-MS Homo sapiens
13 SP110  
Affinity Capture-MS Homo sapiens
14 WNT3A 89780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 USP15 9958
Affinity Capture-MS Homo sapiens
16 PPP2R5C 5527
Affinity Capture-Western Homo sapiens
17 SOGA3  
Affinity Capture-MS Homo sapiens
18 DAPK1 1612
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
19 EIF2AK2 5610
Affinity Capture-Western Homo sapiens
20 APC  
Co-localization Homo sapiens
21 BUB1B  
Affinity Capture-Western Homo sapiens
22 FAM43A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ERGIC3 51614
Affinity Capture-MS Homo sapiens
24 SGOL1  
Affinity Capture-MS Homo sapiens
25 PTTG1  
Biochemical Activity Homo sapiens
26 Soga1  
Affinity Capture-MS Mus musculus
27 CHEK2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
28 C1orf21  
Affinity Capture-MS Homo sapiens
29 MOS 4342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CDK17 5128
Affinity Capture-MS Homo sapiens
31 PRR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 AXIN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
33 NHSL1  
Affinity Capture-MS Homo sapiens
34 NEK1  
Two-hybrid Homo sapiens
35 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
36 C9orf78 51759
Affinity Capture-MS Homo sapiens
37 ERGIC2 51290
Affinity Capture-MS Homo sapiens
38 DDX3X 1654
Co-fractionation Homo sapiens
39 PPP2R1B 5519
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
40 BCL2  
Affinity Capture-Western Homo sapiens
41 MYC  
Affinity Capture-Western Homo sapiens
42 CTLA4  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
43 PPP2R5D 5528
Co-fractionation Homo sapiens
44 SOGA1 140710
Affinity Capture-MS Homo sapiens
45 CBX5 23468
Affinity Capture-MS Homo sapiens
46 Ccng1 12450
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
47 CTNNB1 1499
Co-fractionation Homo sapiens
48 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
49 BRD1 23774
Affinity Capture-MS Homo sapiens
50 MTCL1 23255
Affinity Capture-MS Homo sapiens
51 AIM1 202
Affinity Capture-MS Homo sapiens
52 ITGB7 3695
Affinity Capture-MS Homo sapiens
53 Sgol1  
Affinity Capture-MS Mus musculus
54 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
55 VIM 7431
Co-fractionation Homo sapiens
56 SGOL2 151246
Affinity Capture-MS Homo sapiens
57 PPP2R2D 55844
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 PPP2CB 5516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PPME1 51400
Affinity Capture-MS Homo sapiens
60 CDC27 996
Affinity Capture-Western Homo sapiens
61 KLHL14 57565
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP2R5A is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Costimulation by the CD28 family TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
CTNNB1 S33 mutants aren't phosphorylated TAS Reactome
CTNNB1 S37 mutants aren't phosphorylated TAS Reactome
CTNNB1 S45 mutants aren't phosphorylated TAS Reactome
CTNNB1 T41 mutants aren't phosphorylated TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Interferon Signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
M Phase TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
PKR-mediated signaling TAS Reactome
Platelet homeostasis TAS Reactome
Platelet sensitization by LDL TAS Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by AMER1 mutants TAS Reactome
Signaling by APC mutants TAS Reactome
Signaling by AXIN mutants TAS Reactome
Signaling by CTNNB1 phospho-site mutants TAS Reactome
Signaling by GSK3beta mutants TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Signaling by WNT in cancer TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCF dependent signaling in response to WNT IEA Reactome
Truncations of AMER1 destabilize the destruction complex TAS Reactome





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