Gene description for DAPK1
Gene name death-associated protein kinase 1
Gene symbol DAPK1
Other names/aliases DAPK
Species Homo sapiens
 Database cross references - DAPK1
ExoCarta ExoCarta_1612
Vesiclepedia VP_1612
Entrez Gene 1612
HGNC 2674
MIM 600831
UniProt P53355  
 DAPK1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DAPK1
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 IDA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    ATP binding GO:0005524 IDA
    GTP binding GO:0005525 IEA
    syntaxin-1 binding GO:0017075 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    defense response to tumor cell GO:0002357 IMP
    regulation of response to tumor cell GO:0002834 IDA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 TAS
    apoptotic process GO:0006915 IGI
    apoptotic process GO:0006915 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IEA
    regulation of autophagy GO:0010506 TAS
    positive regulation of autophagy GO:0010508 IMP
    negative regulation of translation GO:0017148 IDA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of apoptotic process GO:0043066 IEA
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 TAS
    cellular response to type II interferon GO:0071346 IDA
    cellular response to hydroperoxide GO:0071447 IMP
    apoptotic signaling pathway GO:0097190 IMP
    positive regulation of autophagic cell death GO:1904094 IMP
    regulation of NMDA receptor activity GO:2000310 ISS
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    postsynaptic density GO:0014069 IEA
    actin cytoskeleton GO:0015629 IDA
    glutamatergic synapse GO:0098978 IEA
    DAPK1-calmodulin complex GO:1990722 IPI
 Experiment description of studies that identified DAPK1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DAPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL20  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
3 CCNDBP1 23582
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 PARP1 142
Proximity Label-MS Homo sapiens
5 SVIL 6840
Affinity Capture-MS Homo sapiens
6 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 TSC2 7249
Affinity Capture-Western Homo sapiens
8 BAG2 9532
Affinity Capture-MS Homo sapiens
9 MAPK1 5594
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 DAPK1 1612
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
11 TMOD3 29766
Affinity Capture-MS Homo sapiens
12 EEF2 1938
Affinity Capture-MS Homo sapiens
13 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ACTC1 70
Affinity Capture-MS Homo sapiens
15 IKBKG 8517
Reconstituted Complex Homo sapiens
16 PDCD6 10016
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 MIB1 57534
Affinity Capture-Western Homo sapiens
18 MAPK3 5595
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 CUL3 8452
Affinity Capture-Western Homo sapiens
20 CAPZA2 830
Affinity Capture-MS Homo sapiens
21 LAMP2 3920
Proximity Label-MS Homo sapiens
22 CALM1 801
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
23 EIF4ENIF1 56478
Two-hybrid Homo sapiens
24 TSC1 7248
Affinity Capture-Western Homo sapiens
25 Mib1  
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
26 CORO1C 23603
Affinity Capture-MS Homo sapiens
27 FADD 8772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
28 ERP44 23071
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CAPZB 832
Affinity Capture-MS Homo sapiens
30 LRRK1 79705
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
32 MYO1D 4642
Affinity Capture-MS Homo sapiens
33 LNX1  
Reconstituted Complex Homo sapiens
34 PPP2R1A 5518
Biochemical Activity Homo sapiens
35 LRRK2 120892
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 DDX5 1655
Affinity Capture-MS Homo sapiens
37 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
38 TWF1 5756
Affinity Capture-MS Homo sapiens
39 KRIT1 889
Affinity Capture-Western Homo sapiens
40 RAB11A 8766
Proximity Label-MS Homo sapiens
41 PPP2CA 5515
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
42 UNC5B 219699
Two-hybrid Homo sapiens
43 YWHAQ 10971
Affinity Capture-Western Homo sapiens
44 BECN1 8678
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
45 PPP2R5A 5525
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
46 TPM3 7170
Affinity Capture-MS Homo sapiens
47 IVNS1ABP  
Affinity Capture-Western Homo sapiens
48 PPP2R2D 55844
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
49 PRRC2B  
Two-hybrid Homo sapiens
50 MDM2  
Protein-peptide Homo sapiens
51 PELI1 57162
Affinity Capture-Western Homo sapiens
52 MYO1B 4430
Affinity Capture-MS Homo sapiens
53 MYL12B 103910
Biochemical Activity Homo sapiens
54 ST13 6767
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 TTLL3 26140
Affinity Capture-MS Homo sapiens
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