Gene ontology annotations for FARSA
Experiment description of studies that identified FARSA in sEVs
1
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
412
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
6
Experiment ID
414
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
7
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
10
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
207
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
208
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
1203
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
15
Experiment ID
407
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
16
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
17
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
18
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
19
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
20
Experiment ID
1275
MISEV standards
Biophysical techniques
✔
Enriched markers
✔
Negative markers
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36706192
Organism
Homo sapiens
Experiment description
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name
Sci Adv
Publication year
2023
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
21
Experiment ID
1280
MISEV standards
Biophysical techniques
✔
Enriched markers
✔
Negative markers
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36706192
Organism
Homo sapiens
Experiment description
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name
Sci Adv
Publication year
2023
Sample
Embryonic kidney cells
Sample name
HEK293T - Fraction 5
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
1.151 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
22
Experiment ID
405
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
23
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
26
Experiment ID
417
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
27
Experiment ID
411
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
28
Experiment ID
488
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
29
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
30
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
31
Experiment ID
413
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
32
Experiment ID
211
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
33
Experiment ID
212
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
34
Experiment ID
406
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
35
Experiment ID
415
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
36
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
37
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
38
Experiment ID
408
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
39
Experiment ID
409
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
40
Experiment ID
416
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
41
Experiment ID
834
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
42
Experiment ID
835
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
43
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
44
Experiment ID
410
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
45
Experiment ID
217
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for FARSA
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
UBE2H
7328
Affinity Capture-MS
Homo sapiens
2
ACOT7
11332
Affinity Capture-MS
Homo sapiens
3
RIT1
6016
Negative Genetic
Homo sapiens
4
ECT2L
Affinity Capture-MS
Homo sapiens
5
SPRTN
Affinity Capture-MS
Homo sapiens
6
COIL
Proximity Label-MS
Homo sapiens
7
NACA
4666
Cross-Linking-MS (XL-MS)
Homo sapiens
8
PRPH
5630
Proximity Label-MS
Homo sapiens
9
APOE
348
Two-hybrid
Homo sapiens
10
UBC
7316
Affinity Capture-MS
Homo sapiens
11
CCT6B
10693
Affinity Capture-MS
Homo sapiens
12
SOX2
Affinity Capture-MS
Homo sapiens
13
ABI3
51225
Two-hybrid
Homo sapiens
14
GRB7
2886
Affinity Capture-MS
Homo sapiens
15
PDCD10
11235
Two-hybrid
Homo sapiens
16
IRF2BP1
26145
Co-fractionation
Homo sapiens
17
KIF23
9493
Affinity Capture-MS
Homo sapiens
18
MCM2
4171
Affinity Capture-MS
Homo sapiens
19
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
20
MAD2L2
10459
Proximity Label-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
21
PRPS1
5631
Co-fractionation
Homo sapiens
22
ASB4
Affinity Capture-MS
Homo sapiens
23
ATG16L1
55054
Affinity Capture-MS
Homo sapiens
24
POLR2C
5432
Proximity Label-MS
Homo sapiens
25
KIAA0368
23392
Co-fractionation
Homo sapiens
26
MECP2
4204
Affinity Capture-MS
Homo sapiens
27
CUL1
8454
Affinity Capture-MS
Homo sapiens
28
ARHGEF15
Affinity Capture-MS
Homo sapiens
29
LAMTOR1
55004
Proximity Label-MS
Homo sapiens
30
WDR1
9948
Co-fractionation
Homo sapiens
31
KRT8
3856
Proximity Label-MS
Homo sapiens
32
NF2
4771
Affinity Capture-MS
Homo sapiens
33
EIF2AK2
5610
Affinity Capture-MS
Homo sapiens
34
DLD
1738
Affinity Capture-MS
Homo sapiens
35
POLD1
5424
Affinity Capture-MS
Homo sapiens
36
GORAB
92344
Two-hybrid
Homo sapiens
37
VAV1
7409
Affinity Capture-MS
Homo sapiens
38
ARHGEF39
84904
Affinity Capture-MS
Homo sapiens
39
RNF32
Two-hybrid
Homo sapiens
40
USP11
8237
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
41
PARK2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
42
A2M
2
Two-hybrid
Homo sapiens
43
PSMD14
10213
Affinity Capture-MS
Homo sapiens
44
PSMG2
56984
Co-fractionation
Homo sapiens
45
CHMP4B
128866
Affinity Capture-MS
Homo sapiens
46
TRIM21
6737
Affinity Capture-MS
Homo sapiens
47
OGT
8473
Co-fractionation
Homo sapiens
Reconstituted Complex
Homo sapiens
48
EEF2
1938
Co-fractionation
Homo sapiens
49
BCL6
Affinity Capture-MS
Homo sapiens
50
TDGF1
Two-hybrid
Homo sapiens
51
HECTD1
25831
Affinity Capture-MS
Homo sapiens
52
RPLP0
6175
Co-fractionation
Homo sapiens
53
COG4
25839
Two-hybrid
Homo sapiens
54
AIMP2
7965
Affinity Capture-MS
Homo sapiens
55
USP32
84669
Affinity Capture-MS
Homo sapiens
56
TMPO
7112
Affinity Capture-MS
Homo sapiens
57
OTUD1
220213
Affinity Capture-MS
Homo sapiens
58
SDHA
6389
Affinity Capture-MS
Homo sapiens
59
RC3H2
Affinity Capture-MS
Homo sapiens
60
NTRK1
4914
Affinity Capture-MS
Homo sapiens
61
CTNNB1
1499
Affinity Capture-MS
Homo sapiens
62
CD274
29126
Affinity Capture-MS
Homo sapiens
63
Rbm14
Two-hybrid
Mus musculus
64
CUL3
8452
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
65
EGFR
1956
Affinity Capture-MS
Homo sapiens
66
FBXO6
26270
Affinity Capture-MS
Homo sapiens
67
RIPK4
Affinity Capture-MS
Homo sapiens
68
CDC42
998
Co-fractionation
Homo sapiens
69
FARSB
10056
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
70
MAPK6
Affinity Capture-MS
Homo sapiens
71
ARHGAP29
9411
Affinity Capture-MS
Homo sapiens
72
RPA3
6119
Proximity Label-MS
Homo sapiens
73
PLEKHG1
Affinity Capture-MS
Homo sapiens
74
XRCC3
Affinity Capture-MS
Homo sapiens
75
PUS1
80324
Co-fractionation
Homo sapiens
76
AKAP1
8165
Proximity Label-MS
Homo sapiens
77
HUWE1
10075
Affinity Capture-MS
Homo sapiens
78
BRAF
Affinity Capture-MS
Homo sapiens
79
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
80
FBXL6
Affinity Capture-MS
Homo sapiens
81
WWOX
51741
Affinity Capture-MS
Homo sapiens
82
ZNF746
Affinity Capture-MS
Homo sapiens
83
SQSTM1
8878
Proximity Label-MS
Homo sapiens
84
UNC93B1
81622
Affinity Capture-MS
Homo sapiens
85
CAPZB
832
Affinity Capture-MS
Homo sapiens
86
MTHFD1
4522
Cross-Linking-MS (XL-MS)
Homo sapiens
87
TARS
6897
Co-fractionation
Homo sapiens
88
ESR1
Reconstituted Complex
Homo sapiens
89
UFL1
23376
Affinity Capture-MS
Homo sapiens
90
WWP2
11060
Affinity Capture-MS
Homo sapiens
91
ARHGAP36
Affinity Capture-MS
Homo sapiens
92
CYLD
Affinity Capture-MS
Homo sapiens
93
KRAS
3845
Negative Genetic
Homo sapiens
94
ARHGAP23
57636
Affinity Capture-MS
Homo sapiens
95
C9orf72
Affinity Capture-MS
Homo sapiens
96
AR
367
Affinity Capture-MS
Homo sapiens
97
RC3H1
149041
Affinity Capture-MS
Homo sapiens
98
ECSIT
51295
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which FARSA is involved