Gene description for PUS1
Gene name pseudouridylate synthase 1
Gene symbol PUS1
Other names/aliases MLASA1
Species Homo sapiens
 Database cross references - PUS1
ExoCarta ExoCarta_80324
Vesiclepedia VP_80324
Entrez Gene 80324
HGNC 15508
MIM 608109
UniProt Q9Y606  
 PUS1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PUS1
Molecular Function
    tRNA binding GO:0000049 IDA
    steroid receptor RNA activator RNA binding GO:0002153 IDA
    RNA binding GO:0003723 HDA
    pseudouridine synthase activity GO:0009982 EXP
    pseudouridine synthase activity GO:0009982 IBA
    pseudouridine synthase activity GO:0009982 IDA
    pseudouridine synthase activity GO:0009982 IMP
    tRNA pseudouridine(38-40) synthase activity GO:0160147 IEA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    tRNA pseudouridine synthesis GO:0031119 IBA
    tRNA pseudouridine synthesis GO:0031119 IMP
    mitochondrial tRNA pseudouridine synthesis GO:0070902 TAS
    mRNA pseudouridine synthesis GO:1990481 IBA
    mRNA pseudouridine synthesis GO:1990481 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified PUS1 in sEVs
1
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PUS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 GTF2F1 2962
Co-fractionation Homo sapiens
3 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
4 NCBP1 4686
Co-fractionation Homo sapiens
5 WDR3  
Co-fractionation Homo sapiens
6 ARPC2 10109
Co-fractionation Homo sapiens
7 PSMD11 5717
Co-fractionation Homo sapiens
8 GADD45A  
Affinity Capture-MS Homo sapiens
9 MTG2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TRMT6 51605
Co-fractionation Homo sapiens
11 PARP8  
Affinity Capture-MS Homo sapiens
12 VPS29 51699
Co-fractionation Homo sapiens
13 FRAT2  
Affinity Capture-MS Homo sapiens
14 LRPPRC 10128
Proximity Label-MS Homo sapiens
15 PPP1R12A 4659
Co-fractionation Homo sapiens
16 EMILIN1 11117
Affinity Capture-MS Homo sapiens
17 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
18 KIF23 9493
Affinity Capture-MS Homo sapiens
19 ACTR3 10096
Co-fractionation Homo sapiens
20 YARS2  
Affinity Capture-MS Homo sapiens
21 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PFDN4 5203
Co-fractionation Homo sapiens
23 MRPL21 219927
Affinity Capture-MS Homo sapiens
24 NARS 4677
Co-fractionation Homo sapiens
25 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
26 MALSU1  
Affinity Capture-MS Homo sapiens
27 EXD2  
Proximity Label-MS Homo sapiens
28 ETNK2  
Affinity Capture-MS Homo sapiens
29 P3H1 64175
Co-fractionation Homo sapiens
30 WDR12 55759
Co-fractionation Homo sapiens
31 MYCN  
Affinity Capture-MS Homo sapiens
32 NMT1 4836
Co-fractionation Homo sapiens
33 FTSJ2 29960
Affinity Capture-MS Homo sapiens
34 KIF3A 11127
Affinity Capture-MS Homo sapiens
35 MDH2 4191
Proximity Label-MS Homo sapiens
36 PCDHB11  
Affinity Capture-MS Homo sapiens
37 FARSA 2193
Co-fractionation Homo sapiens
38 NSUN2 54888
Co-fractionation Homo sapiens
39 IDE 3416
Co-fractionation Homo sapiens
40 C6orf203  
Proximity Label-MS Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 AMACR 23600
Affinity Capture-MS Homo sapiens
43 TUFM 7284
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
44 RHOT2 89941
Proximity Label-MS Homo sapiens
45 PPM1G 5496
Affinity Capture-MS Homo sapiens
46 SP100 6672
Co-fractionation Homo sapiens
47 ZNF664  
Affinity Capture-MS Homo sapiens
48 TRPV5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MRRF  
Proximity Label-MS Homo sapiens
50 THUMPD1 55623
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MTIF2 4528
Proximity Label-MS Homo sapiens
52 UBE2H 7328
Affinity Capture-MS Homo sapiens
53 ECT2 1894
Affinity Capture-MS Homo sapiens
54 MRPS11  
Affinity Capture-MS Homo sapiens
55 PSMD8 5714
Co-fractionation Homo sapiens
56 RMND1 55005
Proximity Label-MS Homo sapiens
57 EGFR 1956
Affinity Capture-MS Homo sapiens
58 FAHD1 81889
Affinity Capture-MS Homo sapiens
59 GATC  
Affinity Capture-MS Homo sapiens
60 HES4  
Affinity Capture-MS Homo sapiens
61 RPA3 6119
Proximity Label-MS Homo sapiens
62 MYC  
Affinity Capture-MS Homo sapiens
63 USP47 55031
Affinity Capture-MS Homo sapiens
64 NIN 51199
Proximity Label-MS Homo sapiens
65 ACAD9 28976
Proximity Label-MS Homo sapiens
66 NMD3 51068
Co-fractionation Homo sapiens
67 Jup 16480
Affinity Capture-MS Mus musculus
68 Nedd1  
Affinity Capture-MS Mus musculus
69 HSPD1 3329
Affinity Capture-MS Homo sapiens
70 NDUFV2 4729
Affinity Capture-MS Homo sapiens
71 PEX3 8504
Proximity Label-MS Homo sapiens
72 PFDN5 5204
Co-fractionation Homo sapiens
73 VPS25 84313
Co-fractionation Homo sapiens
74 AK4 205
Affinity Capture-MS Homo sapiens
75 CS 1431
Proximity Label-MS Homo sapiens
76 MRPL42  
Affinity Capture-MS Homo sapiens
77 AGL 178
Co-fractionation Homo sapiens
78 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
79 FBXW11  
Affinity Capture-MS Homo sapiens
80 TSFM 10102
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
81 AUH 549
Proximity Label-MS Homo sapiens
82 NUP133 55746
Affinity Capture-MS Homo sapiens
83 THUMPD3 25917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 ICT1 3396
Affinity Capture-MS Homo sapiens
85 YBEY  
Affinity Capture-MS Homo sapiens
86 RPA1 6117
Co-fractionation Homo sapiens
87 MEN1 4221
Affinity Capture-MS Homo sapiens
88 ACAA2 10449
Affinity Capture-MS Homo sapiens
89 PDHA1 5160
Proximity Label-MS Homo sapiens
90 PFDN1 5201
Co-fractionation Homo sapiens
91 NDUFS7 374291
Affinity Capture-MS Homo sapiens
92 COX8A  
Proximity Label-MS Homo sapiens
93 C21orf33  
Proximity Label-MS Homo sapiens
94 SSBP1 6742
Proximity Label-MS Homo sapiens
95 PCNA 5111
Co-fractionation Homo sapiens
96 BCAT1 586
Co-fractionation Homo sapiens
97 KLF5  
Affinity Capture-MS Homo sapiens
98 OXLD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 ATP5D 513
Affinity Capture-MS Homo sapiens
100 AARS2  
Proximity Label-MS Homo sapiens
101 PCCB 5096
Affinity Capture-MS Homo sapiens
102 C1QBP 708
Proximity Label-MS Homo sapiens
103 QRSL1  
Affinity Capture-MS Homo sapiens
104 METTL17  
Proximity Label-MS Homo sapiens
105 ABCD4 5826
Affinity Capture-MS Homo sapiens
106 PRMT1 3276
Affinity Capture-MS Homo sapiens
107 NDUFS6  
Affinity Capture-MS Homo sapiens
108 NRD1 4898
Co-fractionation Homo sapiens
View the network image/svg+xml



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