Gene description for PSMG2
Gene name proteasome (prosome, macropain) assembly chaperone 2
Gene symbol PSMG2
Other names/aliases CLAST3
HCCA3
HsT1707
MDS003
PAC2
TNFSF5IP1
Species Homo sapiens
 Database cross references - PSMG2
ExoCarta ExoCarta_56984
Vesiclepedia VP_56984
Entrez Gene 56984
HGNC 24929
MIM 609702
UniProt Q969U7  
 PSMG2 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PSMG2
Molecular Function
    protein binding GO:0005515 IPI
    molecular adaptor activity GO:0060090 IGI
Biological Process
    mitotic spindle assembly checkpoint signaling GO:0007094 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    proteasome assembly GO:0043248 IBA
    chaperone-mediated protein complex assembly GO:0051131 IDA
    chaperone-mediated protein complex assembly GO:0051131 IGI
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    cytosol GO:0005829 IBA
    protein folding chaperone complex GO:0101031 IDA
 Experiment description of studies that identified PSMG2 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PSMG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMB9 5698
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PSMA6 5687
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 PSMB2 5690
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 C6orf15  
Affinity Capture-MS Homo sapiens
6 PSMA2 5683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
7 PSMA8 143471
Affinity Capture-MS Homo sapiens
8 PSMA3 5684
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
9 CA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PSMC3 5702
Co-fractionation Homo sapiens
11 Psmb5 19173
Affinity Capture-MS Mus musculus
12 Psmb4 19172
Affinity Capture-MS Mus musculus
13 SMAD7  
Affinity Capture-MS Homo sapiens
14 MELK  
Affinity Capture-MS Homo sapiens
15 C16orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NOTCH2NL 388677
Two-hybrid Homo sapiens
17 PSMD13 5719
Co-fractionation Homo sapiens
18 PAFAH1B2 5049
Co-fractionation Homo sapiens
19 RPA2 6118
Proximity Label-MS Homo sapiens
20 PSMB7 5695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SOST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 THOP1 7064
Co-fractionation Homo sapiens
23 PSME2 5721
Affinity Capture-MS Homo sapiens
24 POMP  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PSMB4 5692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
26 PSMG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SHFM1 7979
Affinity Capture-MS Homo sapiens
28 EGFR 1956
Negative Genetic Homo sapiens
29 PSMA4 5685
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PRR27 401137
Affinity Capture-MS Homo sapiens
31 SLC35F6 54978
Two-hybrid Homo sapiens
32 LINGO1 84894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SPATA8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PSMD7 5713
Co-fractionation Homo sapiens
35 PSMB8 5696
Affinity Capture-MS Homo sapiens
36 FN1 2335
Affinity Capture-MS Homo sapiens
37 PSMA1 5682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
38 XAGE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CEACAM16  
Affinity Capture-MS Homo sapiens
40 IBSP  
Affinity Capture-MS Homo sapiens
41 PSMG3 84262
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PSME1 5720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 PSMA5 5686
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RPA3 6119
Proximity Label-MS Homo sapiens
45 A1CF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TCF4  
Two-hybrid Homo sapiens
47 PSMA7 5688
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 NRIP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RAB40C 57799
Affinity Capture-MS Homo sapiens
50 PSMF1 9491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PSMC5 5705
Co-fractionation Homo sapiens
53 PSMB5 5693
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
54 KRAS 3845
Negative Genetic Homo sapiens
55 FOXR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 FBXO7 25793
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PSMB6 5694
Co-fractionation Homo sapiens
58 AMPD2 271
Affinity Capture-MS Homo sapiens
59 FAM89B  
Affinity Capture-MS Homo sapiens
60 WARS 7453
Co-fractionation Homo sapiens
61 CCDC22 28952
Co-fractionation Homo sapiens
62 KRT31 3881
Two-hybrid Homo sapiens
63 PSMB1 5689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FARSA 2193
Co-fractionation Homo sapiens
65 PSMG1 8624
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 UCHL5 51377
Affinity Capture-MS Homo sapiens
67 PSMB3 5691
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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