Gene description for PSMF1
Gene name proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
Gene symbol PSMF1
Other names/aliases PI31
Species Homo sapiens
 Database cross references - PSMF1
ExoCarta ExoCarta_9491
Vesiclepedia VP_9491
Entrez Gene 9491
HGNC 9571
UniProt Q92530  
 PSMF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PSMF1
Molecular Function
    endopeptidase inhibitor activity GO:0004866 NAS
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
    protein heterodimerization activity GO:0046982 IPI
    proteasome binding GO:0070628 IDA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    negative regulation of proteasomal protein catabolic process GO:1901799 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    proteasome core complex GO:0005839 NAS
    membrane GO:0016020 HDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified PSMF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PSMF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMB9 5698
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RAB33A 9363
Two-hybrid Homo sapiens
3 PSMA6 5687
Affinity Capture-MS Homo sapiens
4 PSMB2 5690
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 Psmb5 19173
Affinity Capture-MS Mus musculus
6 NUDT21 11051
Two-hybrid Homo sapiens
7 PDLIM7 9260
Two-hybrid Homo sapiens
8 CUL1 8454
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 FASN 2194
Negative Genetic Homo sapiens
11 CBX1 10951
Affinity Capture-MS Homo sapiens
12 TRAF2 7186
Two-hybrid Homo sapiens
13 C9orf24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PSMB3 5691
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FBXO7 25793
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
16 PSMD6 9861
Affinity Capture-MS Homo sapiens
17 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
18 MAGEA11  
Two-hybrid Homo sapiens
19 PSMA4 5685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CTBP2 1488
Two-hybrid Homo sapiens
21 MRPS11  
Affinity Capture-MS Homo sapiens
22 FAM46B  
Two-hybrid Homo sapiens
23 CD2BP2 10421
Two-hybrid Homo sapiens
24 TRIM73  
Two-hybrid Homo sapiens
25 PSMB7 5695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 QKI 9444
Two-hybrid Homo sapiens
27 RALYL 138046
Two-hybrid Homo sapiens
28 SKP1 6500
Affinity Capture-MS Homo sapiens
29 PSMA2 5683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PSMB4 5692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NTNG1  
Affinity Capture-MS Homo sapiens
32 PSMC3  
Reconstituted Complex Bos taurus
33 SHFM1 7979
Affinity Capture-MS Homo sapiens
34 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PSMD4 5710
Affinity Capture-MS Homo sapiens
36 VHL  
Negative Genetic Homo sapiens
37 RNF126  
Two-hybrid Homo sapiens
38 RBFOX1 54715
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
39 HOOK2  
Two-hybrid Homo sapiens
40 BEND7 222389
Two-hybrid Homo sapiens
41 DDX58 23586
Affinity Capture-RNA Homo sapiens
42 RHOXF2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 PSMC1 5700
Affinity Capture-MS Homo sapiens
44 PSMA1 5682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 DVL3 1857
Two-hybrid Homo sapiens
46 PSMG2 56984
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TBK1 29110
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 KHDRBS3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 PSMA2  
Reconstituted Complex Bos taurus
Reconstituted Complex Bos taurus
Co-fractionation Bos taurus
50 PSMG3 84262
Affinity Capture-MS Homo sapiens
51 DDHD1 80821
Affinity Capture-MS Homo sapiens
52 PSMA5 5686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 MIEF2  
Two-hybrid Homo sapiens
54 PSMD4  
Co-fractionation Bos taurus
55 PSMA7 5688
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SEC16A 9919
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
57 CCDC85B  
Two-hybrid Homo sapiens
58 SLC9A3R1 9368
Affinity Capture-MS Homo sapiens
59 PSMF1 9491
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
60 HOXB8  
Affinity Capture-MS Homo sapiens
61 STK11 6794
Negative Genetic Homo sapiens
62 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PSMC1  
Reconstituted Complex Bos taurus
64 SNRNP27  
Affinity Capture-MS Homo sapiens
65 ADAMTS1 9510
Affinity Capture-MS Homo sapiens
66 SHPK 23729
Affinity Capture-MS Homo sapiens
67 PSMD1 5707
Affinity Capture-MS Homo sapiens
68 PSMD3 5709
Affinity Capture-MS Homo sapiens
69 PSMC4 5704
Affinity Capture-MS Homo sapiens
70 ALOX5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 RBMX 27316
Two-hybrid Homo sapiens
72 PSMB1 5689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PSMD8 512891
Reconstituted Complex Bos taurus
74 PSMG1 8624
Affinity Capture-MS Homo sapiens
75 UCHL5 51377
Affinity Capture-MS Homo sapiens
76 PSMC3 5702
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PSMF1 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome





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