ExoCarta: Gene summary

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Gene description for ARHGAP23
Gene name	Rho GTPase activating protein 23
Gene symbol	ARHGAP23
Other names/aliases	-
Species	Homo sapiens
Database cross references - ARHGAP23
ExoCarta	ExoCarta_57636
Vesiclepedia	VP_57636
Entrez Gene	57636
HGNC	29293
MIM	610590
UniProt	Q9P227
ARHGAP23 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Mesenchymal stem cells	Unpublished / Not applicable
Saliva	19199708

Gene ontology annotations for ARHGAP23

Molecular Function
	GTPase activator activity	GO:0005096	TAS
Biological Process
	signal transduction	GO:0007165	IEA
	regulation of small GTPase mediated signal transduction	GO:0051056	TAS
Subcellular Localization
	cytosol	GO:0005829	TAS
	extracellular exosome	GO:0070062	HDA

Experiment description of studies that identified ARHGAP23 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

126

MISEV standards
✘	Biophysical techniques
✔ GAPDH	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [LTQ-FT Ultra]

PubMed ID

Unpublished / Not applicable

Organism

Homo sapiens

Experiment description

Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?

Authors

"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"

Journal name

Mesenchymal Stem Cell Therapy

Publication year

2011

Sample

Mesenchymal stem cells

Sample name

huES9.E1

Isolation/purification methods

HPLC

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Antobody array
Mass spectrometry

Experiment ID

MISEV standards
✔ IEM	Biophysical techniques
✔ TSG101\|Alix\|CD63\|CD81	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

19199708

Organism

Homo sapiens

Experiment description

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

Authors

"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"

Journal name

JPR

Publication year

2009

Sample

Saliva

Sample name

Saliva

Isolation/purification methods

Differential centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy

Protein-protein interactions for ARHGAP23

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

SNRPB2

6629

Affinity Capture-MS

Homo sapiens

PRR36

80164

Affinity Capture-MS

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

RAC1

5879

FRET

Homo sapiens

ANKFY1

51479

Affinity Capture-MS

Homo sapiens

PLEC

5339

Affinity Capture-MS

Homo sapiens

MAT2A

4144

Affinity Capture-MS

Homo sapiens

CCDC86

Affinity Capture-MS

Homo sapiens

HMGB1

3146

Affinity Capture-MS

Homo sapiens

FASTKD1

Affinity Capture-MS

Homo sapiens

EIF6

3692

Affinity Capture-MS

Homo sapiens

YWHAB

7529

Affinity Capture-MS

Homo sapiens

PDIA4

9601

Affinity Capture-MS

Homo sapiens

CAMSAP3

Affinity Capture-MS

Homo sapiens

PLRG1

5356

Affinity Capture-MS

Homo sapiens

DLAT

1737

Affinity Capture-MS

Homo sapiens

LSM14A

26065

Affinity Capture-MS

Homo sapiens

RPAP1

Affinity Capture-MS

Homo sapiens

SNRPA

6626

Affinity Capture-MS

Homo sapiens

BRD2

Affinity Capture-MS

Homo sapiens

SRFBP1

Affinity Capture-MS

Homo sapiens

MEPCE

56257

Affinity Capture-MS

Homo sapiens

HNRNPA1

3178

Affinity Capture-MS

Homo sapiens

AZGP1

563

Affinity Capture-MS

Homo sapiens

KPNA2

3838

Affinity Capture-MS

Homo sapiens

PCNA

5111

Affinity Capture-MS

Homo sapiens

NT5C2

22978

Affinity Capture-MS

Homo sapiens

INA

9118

Affinity Capture-MS

Homo sapiens

CDH1

999

Proximity Label-MS

Homo sapiens

NEFL

4747

Affinity Capture-MS

Homo sapiens

SAPCD1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TFAM

7019

Affinity Capture-MS

Homo sapiens

NOL7

Affinity Capture-MS

Homo sapiens

HMGN1

Affinity Capture-MS

Homo sapiens

SF3B6

51639

Affinity Capture-MS

Homo sapiens

WDR74

54663

Affinity Capture-MS

Homo sapiens

PNMA2

Affinity Capture-MS

Homo sapiens

HP1BP3

50809

Affinity Capture-MS

Homo sapiens

YWHAQ

10971

Affinity Capture-MS

Homo sapiens

FARSA

2193

Affinity Capture-MS

Homo sapiens

ZC3H14

Affinity Capture-MS

Homo sapiens

YWHAG

7532

Affinity Capture-MS

Homo sapiens

Kalrn

Affinity Capture-MS

Rattus norvegicus

RHOA

387

FRET

Homo sapiens

PRDX3

10935

Affinity Capture-MS

Homo sapiens

GSPT1

2935

Affinity Capture-MS

Homo sapiens

TAB1

10454

Affinity Capture-MS

Homo sapiens

IPO8

10526

Affinity Capture-MS

Homo sapiens

CTNNA1

1495

Affinity Capture-MS

Homo sapiens

HMGB2

3148

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which ARHGAP23 is involved

Pathway	Evidence	Source
RAC1 GTPase cycle	TAS	Reactome
RHO GTPase cycle	TAS	Reactome
RHOA GTPase cycle	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

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