Gene description for GRB7
Gene name growth factor receptor-bound protein 7
Gene symbol GRB7
Other names/aliases -
Species Homo sapiens
 Database cross references - GRB7
ExoCarta ExoCarta_2886
Vesiclepedia VP_2886
Entrez Gene 2886
HGNC 4567
MIM 601522
UniProt Q14451  
 GRB7 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GRB7
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IDA
    phosphatidylinositol binding GO:0035091 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    insulin receptor signaling pathway GO:0008286 IBA
    negative regulation of translation GO:0017148 ISS
    positive regulation of cell migration GO:0030335 IDA
    stress granule assembly GO:0034063 ISS
    negative regulation of insulin receptor signaling pathway GO:0046627 IBA
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    focal adhesion GO:0005925 IDA
    cytoplasmic stress granule GO:0010494 ISS
    cell projection GO:0042995 IEA
 Experiment description of studies that identified GRB7 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GRB7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 G3BP2 9908
Affinity Capture-MS Homo sapiens
2 STAC3  
Two-hybrid Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 ZBTB16  
Two-hybrid Homo sapiens
5 CCT7 10574
Affinity Capture-MS Homo sapiens
6 EIF4B 1975
Affinity Capture-MS Homo sapiens
7 KCTD6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
10 HADHB 3032
Two-hybrid Homo sapiens
11 A1BG 1
Two-hybrid Homo sapiens
12 SHC1 6464
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 RBM14 10432
Affinity Capture-MS Homo sapiens
14 RND1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
15 CREBRF  
Two-hybrid Homo sapiens
16 RPS2 6187
Two-hybrid Homo sapiens
17 HSPA8 3312
Affinity Capture-Western Homo sapiens
18 MSH2 4436
Two-hybrid Homo sapiens
19 PKM 5315
Two-hybrid Homo sapiens
20 KMT2B  
Two-hybrid Homo sapiens
21 DOCK7 85440
Two-hybrid Homo sapiens
22 LAX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 EPHB1 2047
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
24 FARSA 2193
Affinity Capture-MS Homo sapiens
25 HDAC2 3066
Affinity Capture-MS Homo sapiens
26 FLAD1 80308
Two-hybrid Homo sapiens
27 SDHA 6389
Affinity Capture-MS Homo sapiens
28 KIT 3815
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
29 FCHO1  
Two-hybrid Homo sapiens
30 DYNC1I1  
Two-hybrid Homo sapiens
31 FGB 2244
Two-hybrid Homo sapiens
32 PTBP1 5725
Affinity Capture-MS Homo sapiens
33 ERBB4 2066
Reconstituted Complex Homo sapiens
34 INSR 3643
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
35 EGFR 1956
Reconstituted Complex Homo sapiens
36 LY6G6F 259215
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
37 CALM1 801
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
38 CAPRIN1 4076
Affinity Capture-Western Homo sapiens
39 PTK2 5747
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
40 PDHB 5162
Two-hybrid Homo sapiens
41 ERBB3 2065
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 SETDB1  
Two-hybrid Homo sapiens
43 HNRNPM 4670
Affinity Capture-MS Homo sapiens
44 AGAP1  
Two-hybrid Homo sapiens
45 STAU1 6780
Affinity Capture-MS Homo sapiens
46 CPNE6 9362
Two-hybrid Homo sapiens
47 PPIL4  
Affinity Capture-MS Homo sapiens
48 RET 5979
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
49 LTV1  
Affinity Capture-MS Homo sapiens
50 GNB2 2783
Two-hybrid Homo sapiens
51 PHAX  
Two-hybrid Homo sapiens
52 TCF12  
Two-hybrid Homo sapiens
53 PUF60 22827
Affinity Capture-MS Homo sapiens
54 MET 4233
Reconstituted Complex Homo sapiens
55 LUC7L3 51747
Affinity Capture-MS Homo sapiens
56 TGM5  
Two-hybrid Homo sapiens
57 RPN1 6184
Affinity Capture-MS Homo sapiens
58 NPM1 4869
Affinity Capture-MS Homo sapiens
59 AR 367
Reconstituted Complex Homo sapiens
60 TMSB4X 7114
Two-hybrid Homo sapiens
61 TLE1 7088
Two-hybrid Homo sapiens
62 FAM124B  
Two-hybrid Homo sapiens
63 TRIM43  
Two-hybrid Homo sapiens
64 ATP5B 506
Two-hybrid Homo sapiens
65 RBM10  
Affinity Capture-MS Homo sapiens
66 PSMD3 5709
Affinity Capture-MS Homo sapiens
67 TRIM36  
Two-hybrid Homo sapiens
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