Gene description for KIT
Gene name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Gene symbol KIT
Other names/aliases C-Kit
CD117
PBT
SCFR
Species Homo sapiens
 Database cross references - KIT
ExoCarta ExoCarta_3815
Vesiclepedia VP_3815
Entrez Gene 3815
HGNC 6342
MIM 164920
UniProt P10721  
 KIT identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for KIT
Molecular Function
    protease binding GO:0002020 IEA
    protein tyrosine kinase activity GO:0004713 TAS
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IBA
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IDA
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    growth factor binding GO:0019838 IBA
    cytokine binding GO:0019955 IDA
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    SH2 domain binding GO:0042169 IEA
    protein homodimerization activity GO:0042803 IPI
    metal ion binding GO:0046872 IEA
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    ovarian follicle development GO:0001541 ISS
    hematopoietic progenitor cell differentiation GO:0002244 IBA
    myeloid progenitor cell differentiation GO:0002318 IEA
    lymphoid progenitor cell differentiation GO:0002320 IEA
    immature B cell differentiation GO:0002327 ISS
    mast cell chemotaxis GO:0002551 IDA
    positive regulation of dendritic cell cytokine production GO:0002732 ISS
    chromatin remodeling GO:0006338 IEA
    glycosphingolipid metabolic process GO:0006687 IEA
    inflammatory response GO:0006954 ISS
    signal transduction GO:0007165 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    spermatogenesis GO:0007283 ISS
    spermatogenesis GO:0007283 TAS
    spermatid development GO:0007286 IEA
    insulin receptor signaling pathway GO:0008286 IEA
    germ cell migration GO:0008354 IEA
    regulation of cell shape GO:0008360 ISS
    visual learning GO:0008542 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    male gonad development GO:0008584 IEP
    cytokine-mediated signaling pathway GO:0019221 IDA
    stem cell population maintenance GO:0019827 TAS
    lamellipodium assembly GO:0030032 ISS
    actin cytoskeleton organization GO:0030036 IDA
    hemopoiesis GO:0030097 TAS
    B cell differentiation GO:0030183 IBA
    T cell differentiation GO:0030217 ISS
    erythrocyte differentiation GO:0030218 ISS
    melanocyte differentiation GO:0030318 ISS
    melanocyte differentiation GO:0030318 TAS
    positive regulation of cell migration GO:0030335 IBA
    positive regulation of pseudopodium assembly GO:0031274 IEA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    positive regulation of mast cell cytokine production GO:0032765 IDA
    somatic stem cell population maintenance GO:0035019 IEA
    embryonic hemopoiesis GO:0035162 ISS
    ectopic germ cell programmed cell death GO:0035234 IEA
    intracellular signal transduction GO:0035556 IEA
    hematopoietic stem cell migration GO:0035701 IEA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    megakaryocyte development GO:0035855 ISS
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Fc receptor signaling pathway GO:0038093 IDA
    Kit signaling pathway GO:0038109 IBA
    Kit signaling pathway GO:0038109 IDA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    erythropoietin-mediated signaling pathway GO:0038162 ISS
    regulation of cell population proliferation GO:0042127 TAS
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 IMP
    negative regulation of programmed cell death GO:0043069 IEA
    mast cell degranulation GO:0043303 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    pigmentation GO:0043473 ISS
    tongue development GO:0043586 IEA
    positive regulation of Notch signaling pathway GO:0045747 IEA
    positive regulation of receptor signaling pathway via JAK-STAT GO:0046427 IBA
    positive regulation of receptor signaling pathway via JAK-STAT GO:0046427 IMP
    response to cadmium ion GO:0046686 IEA
    protein autophosphorylation GO:0046777 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    positive regulation of long-term neuronal synaptic plasticity GO:0048170 IEA
    digestive tract development GO:0048565 ISS
    stem cell differentiation GO:0048863 ISS
    epithelial cell proliferation GO:0050673 IEA
    detection of mechanical stimulus involved in sensory perception of sound GO:0050910 ISS
    positive regulation of DNA-binding transcription factor activity GO:0051091 IMP
    negative regulation of developmental process GO:0051093 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 TAS
    cell chemotaxis GO:0060326 IDA
    mast cell differentiation GO:0060374 ISS
    mast cell differentiation GO:0060374 TAS
    mast cell proliferation GO:0070662 TAS
    positive regulation of mast cell proliferation GO:0070668 IEA
    melanocyte migration GO:0097324 ISS
    melanocyte adhesion GO:0097326 ISS
    positive regulation of pyloric antrum smooth muscle contraction GO:0120072 IEA
    regulation of bile acid metabolic process GO:1904251 IEA
    positive regulation of colon smooth muscle contraction GO:1904343 IEA
    positive regulation of small intestine smooth muscle contraction GO:1904349 IEA
    positive regulation of vascular associated smooth muscle cell differentiation GO:1905065 IDA
    negative regulation of reproductive process GO:2000242 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    acrosomal vesicle GO:0001669 IEA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IEA
    external side of plasma membrane GO:0009897 IEA
    cytoplasmic side of plasma membrane GO:0009898 IEA
    receptor complex GO:0043235 IBA
 Experiment description of studies that identified KIT in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRAP2 9402
Reconstituted Complex Homo sapiens
2 ABL2  
Reconstituted Complex Homo sapiens
3 SHC1 6464
Reconstituted Complex Homo sapiens
4 STAT5B 6777
Biochemical Activity Homo sapiens
5 CRK 1398
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
6 SOCS1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 GRB10 2887
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
8 GRB7 2886
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
9 PTPN20B  
Two-hybrid Homo sapiens
10 PLCG2 5336
Reconstituted Complex Homo sapiens
11 HSH2D 84941
Reconstituted Complex Homo sapiens
12 CTLA4  
Affinity Capture-MS Homo sapiens
13 ZAP70 7535
Reconstituted Complex Homo sapiens
14 FES 2242
Reconstituted Complex Homo sapiens
15 YES1 7525
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 SRC 6714
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
17 PIK3CG  
Co-localization Homo sapiens
18 SYK 6850
Reconstituted Complex Homo sapiens
19 SOCS5  
Reconstituted Complex Homo sapiens
20 PLCG1 5335
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
21 CSF2RA  
Co-localization Homo sapiens
22 SHC4  
Reconstituted Complex Homo sapiens
23 KHDRBS1 10657
Affinity Capture-Western Homo sapiens
24 CLTC 1213
Affinity Capture-Western Homo sapiens
25 MATK  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 SOCS3 9021
Reconstituted Complex Homo sapiens
27 CBLB 868
Affinity Capture-Western Homo sapiens
28 NCK1 4690
Reconstituted Complex Homo sapiens
29 BCAR3 8412
Reconstituted Complex Homo sapiens
30 SMO 6608
Positive Genetic Homo sapiens
31 SOCS6  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 HCK 3055
Reconstituted Complex Homo sapiens
33 HSP90B1 7184
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 LYN 4067
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
35 PSMD14 10213
Affinity Capture-MS Homo sapiens
36 CBL 867
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 TEC 7006
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
38 PTK6  
Reconstituted Complex Homo sapiens
39 RPS6KB1 6198
Negative Genetic Homo sapiens
40 FYN 2534
Affinity Capture-Western Homo sapiens
41 SH2D1A 4068
Reconstituted Complex Homo sapiens
42 CRKL 1399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
43 CD81 975
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 Sh2b2  
Reconstituted Complex Mus musculus
Protein-peptide Mus musculus
45 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
46 SH3BP2  
Reconstituted Complex Homo sapiens
47 CD63 967
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 SH2D2A  
Reconstituted Complex Homo sapiens
49 MPDZ  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
50 STAP1  
Reconstituted Complex Homo sapiens
51 TXK  
Reconstituted Complex Homo sapiens
52 PTPN11 5781
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 SOCS2  
Reconstituted Complex Homo sapiens
54 PTPN6 5777
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 ILKAP 80895
Two-hybrid Homo sapiens
56 SH2D1B  
Reconstituted Complex Homo sapiens
57 SHB 6461
Reconstituted Complex Homo sapiens
58 PDGFRA 5156
Affinity Capture-Western Homo sapiens
59 GRAP  
Reconstituted Complex Homo sapiens
60 CD9 928
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 TNS3 64759
Reconstituted Complex Homo sapiens
62 BLK 640
Reconstituted Complex Homo sapiens
63 IL7R  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
64 STAT1 6772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
65 IFNAR1  
Affinity Capture-MS Homo sapiens
66 KITLG  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
67 PIK3CA 5290
Affinity Capture-Western Homo sapiens
68 TNS2 23371
Reconstituted Complex Homo sapiens
69 SHC3  
Reconstituted Complex Homo sapiens
70 STYX  
Two-hybrid Homo sapiens
71 INPP5D 3635
Reconstituted Complex Homo sapiens
72 IRF3 3661
Affinity Capture-MS Homo sapiens
73 RASA1 5921
Reconstituted Complex Homo sapiens
74 FGR 2268
Reconstituted Complex Homo sapiens
75 SLA2 84174
Reconstituted Complex Homo sapiens
76 GRB2 2885
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
77 TP53 7157
Positive Genetic Homo sapiens
78 USP1 7398
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 SH2D3C 10044
Reconstituted Complex Homo sapiens
80 SH2B3  
Reconstituted Complex Homo sapiens
81 TNS1 7145
Reconstituted Complex Homo sapiens
82 LCK 3932
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
83 ABL1 25
Reconstituted Complex Homo sapiens
84 PTPRO 5800
Affinity Capture-Western Homo sapiens
85 NCK2 8440
Reconstituted Complex Homo sapiens
86 JAK3 3718
Biochemical Activity Homo sapiens
87 DOK1 1796
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
88 PIK3R2 5296
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
89 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
90 BLNK  
Reconstituted Complex Homo sapiens
91 VAV3 10451
Reconstituted Complex Homo sapiens
92 JAK2 3717
Affinity Capture-Western Homo sapiens
93 BCR 613
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
94 PIK3R3 8503
Reconstituted Complex Homo sapiens
95 SHC2 25759
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which KIT is involved
PathwayEvidenceSource
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Dasatinib-resistant KIT mutants TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Drug resistance of KIT mutants TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Imatinib-resistant KIT mutants TAS Reactome
Intracellular signaling by second messengers TAS Reactome
KIT mutants bind TKIs TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Masitinib-resistant KIT mutants TAS Reactome
MITF-M-regulated melanocyte development TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Nilotinib-resistant KIT mutants TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Regorafenib-resistant KIT mutants TAS Reactome
Regulation of KIT signaling TAS Reactome
Regulation of KIT signaling IEA Reactome
RNA Polymerase II Transcription TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by extracellular domain mutants of KIT TAS Reactome
Signaling by juxtamembrane domain KIT mutants TAS Reactome
Signaling by kinase domain mutants of KIT TAS Reactome
Signaling by KIT in disease TAS Reactome
Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT TAS Reactome
Signaling by SCF-KIT IEA Reactome
Sorafenib-resistant KIT mutants TAS Reactome
Sunitinib-resistant KIT mutants TAS Reactome
TFAP2 (AP-2) family regulates transcription of growth factors and their receptors TAS Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity TAS Reactome
Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors TAS Reactome





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