Gene description for GRAP2
Gene name GRB2-related adaptor protein 2
Gene symbol GRAP2
Other names/aliases GADS
GRAP-2
GRB2L
GRBLG
GRID
GRPL
GrbX
Grf40
Mona
P38
Species Homo sapiens
 Database cross references - GRAP2
ExoCarta ExoCarta_9402
Vesiclepedia VP_9402
Entrez Gene 9402
HGNC 4563
MIM 604518
UniProt O75791  
 GRAP2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for GRAP2
Molecular Function
    phosphotyrosine residue binding GO:0001784 IBA
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 IBA
    Ras protein signal transduction GO:0007265 TAS
    cell-cell signaling GO:0007267 TAS
    regulation of MAPK cascade GO:0043408 IBA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified GRAP2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GRAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC7 51434
Affinity Capture-MS Homo sapiens
2 BLNK  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 KHDRBS1 10657
Reconstituted Complex Homo sapiens
4 CD28  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 LAT 27040
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 CCNDBP1 23582
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 ANAPC16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ERBB2 2064
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-localization Homo sapiens
9 RBPMS 11030
Two-hybrid Homo sapiens
10 HEYL  
Affinity Capture-MS Homo sapiens
11 NAGK 55577
Affinity Capture-MS Homo sapiens
12 LAX1  
Affinity Capture-Western Homo sapiens
13 YWHAE 7531
Two-hybrid Homo sapiens
14 ANAPC5 51433
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ANAPC1 64682
Affinity Capture-MS Homo sapiens
16 ALB 213
Affinity Capture-MS Homo sapiens
17 GFAP 2670
Two-hybrid Homo sapiens
18 KIT 3815
Reconstituted Complex Homo sapiens
19 FASLG 356
Reconstituted Complex Homo sapiens
20 SNX18 112574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ERBB4 2066
Reconstituted Complex Homo sapiens
22 CDC23 8697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Reconstituted Complex Homo sapiens
24 ITCH 83737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TRIM68 55128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 BCR 613
Affinity Capture-MS Homo sapiens
27 ERBB3 2065
Reconstituted Complex Homo sapiens
28 GAB2 9846
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 HNRNPK 3190
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 GAB1  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 KRTAP4-12  
Two-hybrid Homo sapiens
32 CBL 867
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
33 ANAPC2 29882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 USP8 9101
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PECR 55825
Affinity Capture-MS Homo sapiens
36 IFNAR2 3455
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TNIP1 10318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ZMYND8 23613
Affinity Capture-MS Homo sapiens
39 LCP2 3937
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
40 ZBTB7B  
Two-hybrid Homo sapiens
41 SOS2 6655
Reconstituted Complex Homo sapiens
42 PTPN23 25930
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ZSCAN21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GNB2L1 10399
Reconstituted Complex Homo sapiens
45 AR 367
Reconstituted Complex Homo sapiens
46 SYK 6850
Affinity Capture-MS Homo sapiens
47 CDC27 996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 STAMBP 10617
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 GAREM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SPRY2 10253
Two-hybrid Homo sapiens
51 CSF1R  
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
52 BEND5  
Two-hybrid Homo sapiens
53 MAP4K1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Phenotypic Enhancement Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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