Gene description for ANAPC5
Gene name anaphase promoting complex subunit 5
Gene symbol ANAPC5
Other names/aliases APC5
Species Homo sapiens
 Database cross references - ANAPC5
ExoCarta ExoCarta_51433
Vesiclepedia VP_51433
Entrez Gene 51433
HGNC 15713
MIM 606948
UniProt Q9UJX4  
 ANAPC5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ANAPC5
Molecular Function
    protein phosphatase binding GO:0019903 IPI
Biological Process
    regulation of mitotic cell cycle GO:0007346 NAS
    anaphase-promoting complex-dependent catabolic process GO:0031145 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IDA
    anaphase-promoting complex-dependent catabolic process GO:0031145 NAS
    positive regulation of mitotic metaphase/anaphase transition GO:0045842 IBA
    cell division GO:0051301 IEA
    regulation of meiotic cell cycle GO:0051445 NAS
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K11-linked ubiquitination GO:0070979 IBA
    protein K11-linked ubiquitination GO:0070979 IDA
    protein branched polyubiquitination GO:0141198 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    anaphase-promoting complex GO:0005680 IBA
    anaphase-promoting complex GO:0005680 IDA
    anaphase-promoting complex GO:0005680 IPI
    anaphase-promoting complex GO:0005680 NAS
    spindle GO:0005819 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ANAPC5 in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ANAPC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRAP2 9402
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CDC26 246184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PABPC1 26986
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 Anapc13  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
5 PAXIP1  
Affinity Capture-MS Homo sapiens
6 Psmb5 19173
Affinity Capture-MS Mus musculus
7 FAM167A  
Affinity Capture-MS Homo sapiens
8 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
9 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
10 PIFO  
Affinity Capture-MS Homo sapiens
11 RPA2 6118
Proximity Label-MS Homo sapiens
12 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 E2F1 1869
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 RPS19 6223
Co-fractionation Homo sapiens
15 TRIM33 51592
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 SNX21  
Affinity Capture-MS Homo sapiens
17 APEX1 328
Affinity Capture-RNA Homo sapiens
18 C9orf78 51759
Affinity Capture-MS Homo sapiens
19 CDC16 8881
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZBTB16  
Two-hybrid Homo sapiens
21 DYRK1A 1859
Affinity Capture-MS Homo sapiens
22 CFAP97  
Affinity Capture-MS Homo sapiens
23 NAA11 84779
Affinity Capture-MS Homo sapiens
24 FZR1  
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CDC27 996
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 FBXO5  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
28 ANAPC7 51434
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 CIB2  
Affinity Capture-MS Homo sapiens
30 ANAPC16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
31 NPAS1  
Affinity Capture-MS Homo sapiens
32 MAD2L1 4085
Affinity Capture-MS Homo sapiens
33 Cdc20  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
34 USP37  
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
35 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 HSPA8 3312
Affinity Capture-MS Homo sapiens
37 FAM96A  
Affinity Capture-MS Homo sapiens
38 SULT1C4  
Affinity Capture-MS Homo sapiens
39 Anapc5 59008
Affinity Capture-MS Mus musculus
40 PLBD1 79887
Affinity Capture-MS Homo sapiens
41 CDC23 8697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
42 RBM28 55131
Affinity Capture-MS Homo sapiens
43 BACH1 571
Two-hybrid Homo sapiens
44 TFDP1  
Affinity Capture-Western Homo sapiens
45 KIAA1683  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CHMP4B 128866
Affinity Capture-MS Homo sapiens
47 NEK2  
Affinity Capture-MS Homo sapiens
48 Gm9174  
Affinity Capture-MS Mus musculus
49 TTLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ANAPC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ANAPC4 29945
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
52 VCPIP1 80124
Affinity Capture-MS Homo sapiens
53 LATS2 26524
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
54 HSPB8 26353
Affinity Capture-MS Homo sapiens
55 CDT1  
Affinity Capture-MS Homo sapiens
56 UBXN6 80700
Affinity Capture-MS Homo sapiens
57 ID2  
Reconstituted Complex Homo sapiens
58 UBE2S  
Reconstituted Complex Homo sapiens
59 ANAPC13 25847
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ANO6 196527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 Cdc23  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
62 SMARCAD1  
Affinity Capture-MS Homo sapiens
63 ATL3 25923
Affinity Capture-MS Homo sapiens
64 TP53BP1 7158
Affinity Capture-MS Homo sapiens
65 XPO1 7514
Affinity Capture-MS Homo sapiens
66 BAG2 9532
Affinity Capture-MS Homo sapiens
67 NCAPH2  
Affinity Capture-MS Homo sapiens
68 ANAPC10  
Affinity Capture-MS Homo sapiens
69 RAD51B  
Affinity Capture-MS Homo sapiens
70 CUL3 8452
Affinity Capture-MS Homo sapiens
71 PTEN 5728
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
72 EGFR 1956
Negative Genetic Homo sapiens
73 CUL9  
Affinity Capture-MS Homo sapiens
74 DUSP16  
Affinity Capture-MS Homo sapiens
75 GTF2E1 2960
Co-fractionation Homo sapiens
76 Anapc16  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
77 Cdc16  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
78 FKBP5 2289
Affinity Capture-MS Homo sapiens
79 MAPK6  
Two-hybrid Homo sapiens
80 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
81 HECW2  
Affinity Capture-MS Homo sapiens
82 KNG1 3827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 Anapc1  
Affinity Capture-MS Mus musculus
84 Bub1b 12236
Affinity Capture-MS Mus musculus
85 SF3B3 23450
Affinity Capture-MS Homo sapiens
86 Mad2l1 56150
Affinity Capture-MS Mus musculus
87 BRD3 8019
Affinity Capture-MS Homo sapiens
88 HUWE1 10075
Affinity Capture-MS Homo sapiens
89 Anapc2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
90 TRAK2 66008
Affinity Capture-MS Homo sapiens
91 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
92 CDC20 991
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 SIRT6  
Affinity Capture-MS Homo sapiens
94 BUB1B  
Affinity Capture-MS Homo sapiens
95 SKP1 6500
Affinity Capture-MS Homo sapiens
96 PHF8  
Affinity Capture-MS Homo sapiens
97 ANAPC1 64682
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
98 CREBBP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
99 MAGIX 79917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 ANAPC2 29882
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 C6orf141  
Affinity Capture-MS Homo sapiens
102 PPP4R1L  
Affinity Capture-MS Homo sapiens
103 PENK 5179
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 PSMA5 5686
Affinity Capture-MS Homo sapiens
105 INF2 64423
Affinity Capture-MS Homo sapiens
106 C16orf72 29035
Affinity Capture-MS Homo sapiens
107 L3MBTL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 BRD1 23774
Affinity Capture-MS Homo sapiens
109 SERBP1 26135
Affinity Capture-MS Homo sapiens
110 KRAS 3845
Negative Genetic Homo sapiens
111 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
112 SRP9 6726
Affinity Capture-MS Homo sapiens
113 CCT8L2  
Affinity Capture-MS Homo sapiens
114 RPA3 6119
Proximity Label-MS Homo sapiens
115 C16orf87 388272
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 NOTCH1 4851
Affinity Capture-MS Homo sapiens
117 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
118 GALNT9  
Affinity Capture-MS Homo sapiens
119 CUL4A 8451
Affinity Capture-MS Homo sapiens
120 EP300 2033
Affinity Capture-Western Homo sapiens
121 Cdc26  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which ANAPC5 is involved
PathwayEvidenceSource
Aberrant regulation of mitotic cell cycle due to RB1 defects TAS Reactome
Aberrant regulation of mitotic exit in cancer due to RB1 defects TAS Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Adaptive Immune System TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC-Cdc20 mediated degradation of Nek2A TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint TAS Reactome
Assembly of the pre-replicative complex TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase TAS Reactome
Disease TAS Reactome
Diseases of mitotic cell cycle TAS Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Immune System TAS Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex TAS Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components TAS Reactome
M Phase TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Phosphorylation of the APC/C TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of mitotic cell cycle TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Separation of Sister Chromatids TAS Reactome
Switching of origins to a post-replicative state TAS Reactome
Synthesis of DNA TAS Reactome
Transcriptional Regulation by VENTX TAS Reactome





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