Gene description for GTF2E1
Gene name general transcription factor IIE, polypeptide 1, alpha 56kDa
Gene symbol GTF2E1
Other names/aliases FE
TF2E1
TFIIE-A
Species Homo sapiens
 Database cross references - GTF2E1
ExoCarta ExoCarta_2960
Vesiclepedia VP_2960
Entrez Gene 2960
HGNC 4650
MIM 189962
UniProt P29083  
 GTF2E1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GTF2E1
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    transcription by RNA polymerase II GO:0006366 IDA
    transcription initiation at RNA polymerase II promoter GO:0006367 IBA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIIE complex GO:0005673 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified GTF2E1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GTF2E1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 AARSD1 80755
Co-fractionation Homo sapiens
3 CDC14B 8555
Proximity Label-MS Homo sapiens
4 SNF8 11267
Co-fractionation Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 TAF6 6878
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 GTF2F1 2962
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
8 TAF7  
Co-fractionation Homo sapiens
9 ZBTB9  
Two-hybrid Homo sapiens
10 GTF2A1 2957
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
11 POLR2H 5437
Co-fractionation Homo sapiens
12 PCBD2 84105
Two-hybrid Homo sapiens
13 TTC1 7265
Co-fractionation Homo sapiens
14 PARP1 142
Proximity Label-MS Homo sapiens
15 MED17  
Affinity Capture-MS Homo sapiens
16 CDK7 1022
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
17 Gtf2e2  
Affinity Capture-MS Mus musculus
18 TRIM15  
Two-hybrid Homo sapiens
19 SRRM1 10250
Co-fractionation Homo sapiens
20 TP53 7157
Co-fractionation Homo sapiens
21 SLC45A2 51151
Affinity Capture-MS Homo sapiens
22 TBC1D21 161514
Two-hybrid Homo sapiens
23 POLR2G 5436
Co-fractionation Homo sapiens
24 SRPK2 6733
Biochemical Activity Homo sapiens
25 FCGRT 2217
Affinity Capture-MS Homo sapiens
26 AES 166
Reconstituted Complex Homo sapiens
27 FAS 355
Proximity Label-MS Homo sapiens
28 SMARCC1 6599
Co-fractionation Homo sapiens
29 TRIM24  
Biochemical Activity Homo sapiens
30 POLR2E 5434
Co-fractionation Homo sapiens
31 NSUN2 54888
Co-fractionation Homo sapiens
32 ACTC1 70
Proximity Label-MS Homo sapiens
33 BRCA1 672
Co-purification Homo sapiens
34 ATF7IP 55729
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
35 IKBKB 3551
Affinity Capture-MS Homo sapiens
36 CBX3 11335
Proximity Label-MS Homo sapiens
37 WDR70 55100
Co-fractionation Homo sapiens
38 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
39 POLR2K  
Co-fractionation Homo sapiens
40 CHUK 1147
Affinity Capture-MS Homo sapiens
41 TAF1  
Reconstituted Complex Homo sapiens
42 NUP50 10762
Proximity Label-MS Homo sapiens
43 POLR2A 5430
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
44 GTF2B 2959
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
45 APP 351
Reconstituted Complex Homo sapiens
46 SH3GL1 6455
Co-fractionation Homo sapiens
47 TAF11  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
48 TBP  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
49 TCEA1 6917
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
50 EFTUD2 9343
Co-fractionation Homo sapiens
51 ERCC3  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
52 DDX23 9416
Proximity Label-MS Homo sapiens
53 MED21  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
54 CBFA2T2  
Two-hybrid Homo sapiens
55 XPO5 57510
Co-fractionation Homo sapiens
56 GTF2H1 2965
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
57 DRAP1 10589
Co-fractionation Homo sapiens
58 GTF2E2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
59 OGT 8473
Reconstituted Complex Homo sapiens
60 HARS 3035
Co-fractionation Homo sapiens
61 EEF1G 1937
Co-fractionation Homo sapiens
62 POLR2B 5431
Co-fractionation Homo sapiens
63 ZSCAN1  
Two-hybrid Homo sapiens
64 SMARCB1 6598
Co-fractionation Homo sapiens
65 SH3GLB2 56904
Co-fractionation Homo sapiens
66 GTF2H4 2968
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
67 SARS 6301
Co-fractionation Homo sapiens
68 SMARCA2 6595
Co-fractionation Homo sapiens
69 NRARP  
Two-hybrid Homo sapiens
70 POLR1E  
Proximity Label-MS Homo sapiens
71 TRIM29 23650
Two-hybrid Homo sapiens
72 RPUSD2  
Co-fractionation Homo sapiens
73 LMNA 4000
Proximity Label-MS Homo sapiens
74 SND1 27044
Reconstituted Complex Homo sapiens
75 OXR1  
Co-fractionation Homo sapiens
76 SETD7 80854
Co-fractionation Homo sapiens
77 COIL  
Proximity Label-MS Homo sapiens
78 ANAPC5 51433
Co-fractionation Homo sapiens
79 SMARCC2 6601
Co-fractionation Homo sapiens
80 IKBKG 8517
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
81 ACBD3 64746
Co-fractionation Homo sapiens
82 GTF2F2 2963
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
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