Gene description for Anapc5
Gene name anaphase-promoting complex subunit 5
Gene symbol Anapc5
Other names/aliases 2510006G12Rik
AA408751
AA536819
AA986414
APC5
Anpc5
Species Mus musculus
 Database cross references - Anapc5
ExoCarta ExoCarta_59008
Vesiclepedia VP_59008
Entrez Gene 59008
UniProt Q8BTZ4  
 Anapc5 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Anapc5
Molecular Function
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
Biological Process
    anaphase-promoting complex-dependent catabolic process GO:0031145 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 ISO
    anaphase-promoting complex-dependent catabolic process GO:0031145 ISS
    positive regulation of mitotic metaphase/anaphase transition GO:0045842 IBA
    cell division GO:0051301 IEA
    protein K48-linked ubiquitination GO:0070936 ISO
    protein K48-linked ubiquitination GO:0070936 ISS
    protein K11-linked ubiquitination GO:0070979 IBA
    protein K11-linked ubiquitination GO:0070979 ISO
    protein K11-linked ubiquitination GO:0070979 ISS
    protein branched polyubiquitination GO:0141198 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    anaphase-promoting complex GO:0005680 IBA
    anaphase-promoting complex GO:0005680 ISO
    anaphase-promoting complex GO:0005680 ISS
    cytoplasm GO:0005737 IEA
    spindle GO:0005819 ISO
    spindle GO:0005819 ISS
 Experiment description of studies that identified Anapc5 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Anapc5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC7 51434
Affinity Capture-MS Homo sapiens
2 FIP1L1 81608
Affinity Capture-MS Homo sapiens
3 ANAPC16  
Affinity Capture-MS Homo sapiens
4 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
5 MAP3K3 4215
Affinity Capture-MS Homo sapiens
6 CDC26 246184
Affinity Capture-MS Homo sapiens
7 ANAPC4 29945
Affinity Capture-MS Homo sapiens
8 WDR33 55339
Affinity Capture-MS Homo sapiens
9 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
10 CDC23 8697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CDC16 8881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ABL1 25
Affinity Capture-MS Homo sapiens
13 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
14 ANAPC1 64682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CDC20 991
Affinity Capture-MS Homo sapiens
16 Chaf1a  
Affinity Capture-MS Mus musculus
17 ANAPC2 29882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 BUB1B  
Affinity Capture-MS Homo sapiens
19 CDC27 996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ANAPC5 51433
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Anapc5 is involved
PathwayEvidenceSource
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC-Cdc20 mediated degradation of Nek2A IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of Cyclin B IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Assembly of the pre-replicative complex IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase IEA Reactome
DNA Replication IEA Reactome
DNA Replication Pre-Initiation IEA Reactome
Immune System IEA Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex IEA Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components IEA Reactome
M Phase IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Phosphorylation of the APC/C IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
S Phase IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Separation of Sister Chromatids IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome





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