Gene description for CRYL1
Gene name crystallin, lambda 1
Gene symbol CRYL1
Other names/aliases GDH
HEL30
lambda-CRY
Species Homo sapiens
 Database cross references - CRYL1
ExoCarta ExoCarta_51084
Vesiclepedia VP_51084
Entrez Gene 51084
HGNC 18246
MIM 609877
UniProt Q9Y2S2  
 CRYL1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Urine 19056867    
 Gene ontology annotations for CRYL1
Molecular Function
    protein homodimerization activity GO:0042803 IPI
    L-gulonate 3-dehydrogenase activity GO:0050104 IBA
    L-gulonate 3-dehydrogenase activity GO:0050104 IDA
    NAD+ binding GO:0070403 IDA
Biological Process
    fatty acid metabolic process GO:0006631 IEA
    D-glucuronate catabolic process to D-xylulose 5-phosphate GO:0019640 IEA
Subcellular Localization
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CRYL1 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CRYL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C5orf34  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
3 ABCC6  
Two-hybrid Homo sapiens
4 BRIP1  
Affinity Capture-MS Homo sapiens
5 AP2B1 163
Affinity Capture-MS Homo sapiens
6 HOOK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SMG7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 IFT140 9742
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 WRAP53  
Affinity Capture-MS Homo sapiens
10 MYL12B 103910
Affinity Capture-MS Homo sapiens
11 ALMS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SMG8 55181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ACAA1 30
Affinity Capture-MS Homo sapiens
14 UBR4 23352
Affinity Capture-MS Homo sapiens
15 TTC37 9652
Affinity Capture-MS Homo sapiens
16 ANAPC5 51433
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 F2R 2149
Affinity Capture-MS Homo sapiens
18 IFT122 55764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TRIM65 201292
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NR3C1 2908
Proximity Label-MS Homo sapiens
21 CDC23 8697
Affinity Capture-MS Homo sapiens
22 ANAPC1 64682
Affinity Capture-MS Homo sapiens
23 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
24 MYO5C 55930
Affinity Capture-MS Homo sapiens
25 COPE 11316
Affinity Capture-MS Homo sapiens
26 TBCD 6904
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 HTT 3064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TUBA4A 7277
Affinity Capture-MS Homo sapiens
29 HAUS5 23354
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SPG11 80208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 BRCA2 675
Affinity Capture-MS Homo sapiens
32 EIF2AK4 440275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MYO9A 4649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 FAM57A  
Affinity Capture-MS Homo sapiens
35 USP19 10869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CTNNB1 1499
Affinity Capture-MS Homo sapiens
37 WDR59 79726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CEP164  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TRIM32 22954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PKD2 5311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MTMR1 8776
Affinity Capture-MS Homo sapiens
42 FLII 2314
Affinity Capture-MS Homo sapiens
43 STIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GNAI2 2771
Affinity Capture-MS Homo sapiens
45 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HSPA9 3313
Affinity Capture-MS Homo sapiens
47 DDX39B 7919
Affinity Capture-MS Homo sapiens
48 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PLD2 5338
Affinity Capture-MS Homo sapiens
50 HAUS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 USP54 159195
Affinity Capture-MS Homo sapiens
52 KIAA1919  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CRYL1 is involved
PathwayEvidenceSource
Formation of xylulose-5-phosphate TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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