Gene description for LCP2
Gene name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Gene symbol LCP2
Other names/aliases SLP-76
SLP76
Species Homo sapiens
 Database cross references - LCP2
ExoCarta ExoCarta_3937
Vesiclepedia VP_3937
Entrez Gene 3937
HGNC 6529
MIM 601603
UniProt Q13094  
 LCP2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for LCP2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    immune response GO:0006955 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    intracellular signal transduction GO:0035556 IBA
    mast cell activation GO:0045576 IEA
    positive regulation of protein kinase activity GO:0045860 IEP
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cell-cell junction GO:0005911 IEA
    TCR signalosome GO:0036398 IDA
    plasma membrane raft GO:0044853 IDA
 Experiment description of studies that identified LCP2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LCP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRAP2 9402
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
2 YBX1 4904
Two-hybrid Homo sapiens
3 LYN 4067
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 Fyb  
Affinity Capture-Western Mus musculus
5 NCK1 4690
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
7 SHC1 6464
Affinity Capture-Western Homo sapiens
8 PTPN6 5777
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 TRAT1 50852
Reconstituted Complex Homo sapiens
10 YWHAE 7531
Reconstituted Complex Homo sapiens
11 STAM2 10254
Two-hybrid Homo sapiens
12 VAV1 7409
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 YWHAZ 7534
Two-hybrid Homo sapiens
14 ERBB4 2066
Reconstituted Complex Homo sapiens
15 SHB 6461
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
17 ERBB3 2065
Reconstituted Complex Homo sapiens
18 PIK3R1 5295
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 PRAM1  
Affinity Capture-Western Homo sapiens
21 NCK2 8440
Two-hybrid Homo sapiens
22 FYB 2533
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 GOLGA2 2801
Two-hybrid Homo sapiens
25 WAS 7454
Affinity Capture-Western Homo sapiens
26 PAG1 55824
Reconstituted Complex Homo sapiens
27 FXR2 9513
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 ZAP70 7535
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
29 ERBB2 2064
Reconstituted Complex Homo sapiens
30 ITK 3702
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
31 SYK 6850
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 PLCG1 5335
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
33 SH3GL2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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