Gene description for DOK1
Gene name docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Gene symbol DOK1
Other names/aliases P62DOK
Species Homo sapiens
 Database cross references - DOK1
ExoCarta ExoCarta_1796
Vesiclepedia VP_1796
Entrez Gene 1796
HGNC 2990
MIM 602919
UniProt Q99704  
 DOK1 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for DOK1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    Ras protein signal transduction GO:0007265 IBA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified DOK1 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 COLGALT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CD28  
Affinity Capture-Western Homo sapiens
4 DOK2 9046
Affinity Capture-Western Homo sapiens
5 ABL2  
Protein-peptide Homo sapiens
6 NCK1 4690
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
7 FGR 2268
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
8 GRB2 2885
Affinity Capture-Western Homo sapiens
9 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
10 CBLL1  
Affinity Capture-Western Homo sapiens
11 CRK 1398
Protein-peptide Homo sapiens
12 KIT 3815
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 VAV1 7409
Protein-peptide Homo sapiens
14 ABL1 25
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
15 INSR 3643
Biochemical Activity Homo sapiens
16 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
17 Nck1  
Affinity Capture-Western Rattus norvegicus
18 RET 5979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 LYN 4067
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
20 NCK2 8440
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TEC 7006
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
22 YES1 7525
Reconstituted Complex Homo sapiens
23 PTK6  
Protein-peptide Homo sapiens
24 DOK1 1796
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 HCK 3055
Reconstituted Complex Homo sapiens
26 SH2D1A 4068
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 CRKL 1399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
28 ERBB2 2064
Protein-peptide Homo sapiens
29 SRC 6714
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
30 PIK3R1 5295
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
31 INPP5D 3635
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 PLCG1 5335
Reconstituted Complex Homo sapiens
33 Syngap1  
Affinity Capture-Western Rattus norvegicus
34 ITK 3702
Protein-peptide Homo sapiens
35 BCR 613
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here