Gene description for TEC
Gene name tec protein tyrosine kinase
Gene symbol TEC
Other names/aliases PSCTK4
Species Homo sapiens
 Database cross references - TEC
ExoCarta ExoCarta_7006
Vesiclepedia VP_7006
Entrez Gene 7006
HGNC 11719
MIM 600583
UniProt P42680  
 TEC identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for TEC
Molecular Function
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    phospholipid binding GO:0005543 NAS
    histone H3Y41 kinase activity GO:0035401 IEA
    metal ion binding GO:0046872 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    adaptive immune response GO:0002250 IBA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    integrin-mediated signaling pathway GO:0007229 IDA
    regulation of platelet activation GO:0010543 IDA
    intracellular signal transduction GO:0035556 IEA
    tissue regeneration GO:0042246 IEA
    T cell receptor signaling pathway GO:0050852 IBA
    B cell receptor signaling pathway GO:0050853 IBA
    B cell receptor signaling pathway GO:0050853 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified TEC in sEVs
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TEC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRRG4  
Reconstituted Complex Homo sapiens
2 ARHGEF12 23365
Phenotypic Enhancement Homo sapiens
Biochemical Activity Homo sapiens
3 GNA12 2768
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
4 ERBB2 2064
Protein-peptide Homo sapiens
5 GRB2 2885
Affinity Capture-MS Homo sapiens
6 SHC1 6464
Affinity Capture-MS Homo sapiens
7 KIT 3815
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 FASLG 356
Protein-peptide Homo sapiens
9 VAV1 7409
Affinity Capture-Western Homo sapiens
10 ERBB4 2066
Reconstituted Complex Homo sapiens
11 SOCS1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
12 EGFR 1956
Reconstituted Complex Homo sapiens
13 ERBB3 2065
Reconstituted Complex Homo sapiens
14 WAS 7454
Reconstituted Complex Homo sapiens
15 LYN 4067
Affinity Capture-Western Homo sapiens
16 GAB1  
Reconstituted Complex Homo sapiens
17 RPE  
Co-fractionation Homo sapiens
18 DOK1 1796
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
19 JAK1 3716
Affinity Capture-Western Homo sapiens
20 Nedd4 25489
Biochemical Activity Rattus norvegicus
21 SORT1 6272
Affinity Capture-MS Homo sapiens
22 PIK3R2 5296
Two-hybrid Homo sapiens
23 MET 4233
Reconstituted Complex Homo sapiens
24 PRLR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 GNA13 10672
Phenotypic Enhancement Homo sapiens
26 RAC1 5879
Phenotypic Enhancement Homo sapiens
27 JAK2 3717
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
28 IKBKG 8517
Reconstituted Complex Homo sapiens
29 MED28  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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