Gene description for LIG1
Gene name ligase I, DNA, ATP-dependent
Gene symbol LIG1
Other names/aliases -
Species Homo sapiens
 Database cross references - LIG1
ExoCarta ExoCarta_3978
Vesiclepedia VP_3978
Entrez Gene 3978
HGNC 6598
MIM 126391
UniProt P18858  
 LIG1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for LIG1
Molecular Function
    DNA binding GO:0003677 IEA
    DNA ligase activity GO:0003909 IDA
    DNA ligase activity GO:0003909 IMP
    DNA ligase activity GO:0003909 TAS
    DNA ligase (ATP) activity GO:0003910 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    DNA ligation GO:0006266 IBA
    DNA ligation GO:0006266 TAS
    lagging strand elongation GO:0006273 IBA
    DNA repair GO:0006281 TAS
    base-excision repair GO:0006284 IDA
    base-excision repair, gap-filling GO:0006287 TAS
    mismatch repair GO:0006298 TAS
    anatomical structure morphogenesis GO:0009653 TAS
    V(D)J recombination GO:0033151 IDA
    cell division GO:0051301 IEA
    DNA biosynthetic process GO:0071897 IEA
    Okazaki fragment processing involved in mitotic DNA replication GO:1903461 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified LIG1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LIG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 SPANXN5  
Affinity Capture-MS Homo sapiens
4 PRIM2 5558
Co-fractionation Homo sapiens
5 UBE3B 89910
Affinity Capture-MS Homo sapiens
6 PRMT3 10196
Co-fractionation Homo sapiens
7 PPP3CA 5530
Co-fractionation Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 USP48  
Co-fractionation Homo sapiens
10 ZBTB2 57621
Affinity Capture-MS Homo sapiens
11 PPP1CB 5500
Co-fractionation Homo sapiens
12 STKLD1  
Co-fractionation Homo sapiens
13 CCNA2 890
Co-fractionation Homo sapiens
14 PARP1 142
Co-fractionation Homo sapiens
15 CLTC 1213
Co-fractionation Homo sapiens
16 PIP4K2C 79837
Co-fractionation Homo sapiens
17 JUP 3728
Co-fractionation Homo sapiens
18 FEN1 2237
Co-fractionation Homo sapiens
19 SYT6  
Affinity Capture-MS Homo sapiens
20 BAG2 9532
Affinity Capture-MS Homo sapiens
21 RUVBL2 10856
Co-fractionation Homo sapiens
22 RBMX 27316
Co-fractionation Homo sapiens
23 ARHGEF1 9138
Co-fractionation Homo sapiens
24 S100A6 6277
Affinity Capture-MS Homo sapiens
25 MCM5 4174
Co-fractionation Homo sapiens
26 AURKA 6790
Affinity Capture-MS Homo sapiens
27 RPS12 6206
Co-fractionation Homo sapiens
28 CHTF18 63922
Co-fractionation Homo sapiens
29 RPA1 6117
Co-fractionation Homo sapiens
30 USP5 8078
Co-fractionation Homo sapiens
31 POLE3  
Co-fractionation Homo sapiens
32 POLD1 5424
Co-fractionation Homo sapiens
33 PDHA1 5160
Co-fractionation Homo sapiens
34 TCAF1 9747
Co-fractionation Homo sapiens
35 RGS2  
Two-hybrid Homo sapiens
36 RPL9 6133
Co-fractionation Homo sapiens
37 HSPB1 3315
Two-hybrid Homo sapiens
38 CLUH 23277
Co-fractionation Homo sapiens
39 FAM35A  
Proximity Label-MS Homo sapiens
40 PPP1R7 5510
Co-fractionation Homo sapiens
41 CSNK1A1 1452
Co-fractionation Homo sapiens
42 NT5DC2 64943
Co-fractionation Homo sapiens
43 RFC1 5981
Co-fractionation Homo sapiens
44 PCNA 5111
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
45 MRE11A 4361
Two-hybrid Homo sapiens
46 APEX1 328
Proximity Label-MS Homo sapiens
47 POLD3  
Co-fractionation Homo sapiens
48 CBX3 11335
Proximity Label-MS Homo sapiens
49 CDK2 1017
Co-fractionation Homo sapiens
50 RPA3 6119
Co-fractionation Homo sapiens
51 HS1BP3 64342
Co-fractionation Homo sapiens
52 Lyplal1  
Affinity Capture-MS Mus musculus
53 Tgs1  
Affinity Capture-MS Mus musculus
54 MOV10 4343
Affinity Capture-RNA Homo sapiens
55 UBA3 9039
Co-fractionation Homo sapiens
56 RPA2 6118
Co-fractionation Homo sapiens
57 BBS1 582
Affinity Capture-MS Homo sapiens
58 DYRK1A 1859
Affinity Capture-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PDHB 5162
Co-fractionation Homo sapiens
62 U2AF2 11338
Co-fractionation Homo sapiens
63 TOP1 7150
Co-fractionation Homo sapiens
64 HSPA1A 3303
Affinity Capture-MS Homo sapiens
65 HSPA4 3308
Co-fractionation Homo sapiens
66 POLA1  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
67 SKIV2L2 23517
Co-fractionation Homo sapiens
68 HSPA4L 22824
Co-fractionation Homo sapiens
69 UHRF1 29128
Protein-peptide Homo sapiens
70 PRIM1  
Co-fractionation Homo sapiens
71 NCAN  
Co-fractionation Homo sapiens
72 POLE  
Co-fractionation Homo sapiens
73 POLA2  
Co-fractionation Homo sapiens
74 GSPT1 2935
Co-fractionation Homo sapiens
75 POLD2 5425
Co-fractionation Homo sapiens
76 CEBPA  
Protein-peptide Homo sapiens
77 BIN1 274
Co-fractionation Homo sapiens
78 NAE1 8883
Co-fractionation Homo sapiens
79 EIF4B 1975
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which LIG1 is involved
PathwayEvidenceSource
Base Excision Repair TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Chromosome Maintenance TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
DNA Repair TAS Reactome
DNA Replication TAS Reactome
DNA strand elongation TAS Reactome
Early Phase of HIV Life Cycle TAS Reactome
Extension of Telomeres TAS Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER TAS Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Global Genome Nucleotide Excision Repair (GG-NER) TAS Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Infectious disease TAS Reactome
Lagging Strand Synthesis TAS Reactome
Mismatch Repair TAS Reactome
Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) TAS Reactome
Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) TAS Reactome
MITF-M-dependent gene expression TAS Reactome
MITF-M-regulated melanocyte development TAS Reactome
Nucleotide Excision Repair TAS Reactome
PCNA-Dependent Long Patch Base Excision Repair TAS Reactome
POLB-Dependent Long Patch Base Excision Repair TAS Reactome
Processive synthesis on the C-strand of the telomere TAS Reactome
Processive synthesis on the lagging strand TAS Reactome
Regulation of MITF-M-dependent genes involved in DNA damage repair and senescence TAS Reactome
Resolution of Abasic Sites (AP sites) TAS Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway TAS Reactome
S Phase TAS Reactome
Synthesis of DNA TAS Reactome
Telomere C-strand (Lagging Strand) Synthesis TAS Reactome
Telomere Maintenance TAS Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Viral Infection Pathways TAS Reactome





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