Gene description for NAE1
Gene name NEDD8 activating enzyme E1 subunit 1
Gene symbol NAE1
Other names/aliases A-116A10.1
APPBP1
HPP1
ula-1
Species Homo sapiens
 Database cross references - NAE1
ExoCarta ExoCarta_8883
Vesiclepedia VP_8883
Entrez Gene 8883
HGNC 621
MIM 603385
UniProt Q13564  
 NAE1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for NAE1
Molecular Function
    protein binding GO:0005515 IPI
    NEDD8 activating enzyme activity GO:0019781 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    signal transduction GO:0007165 TAS
    mitotic DNA replication checkpoint signaling GO:0033314 IDA
    regulation of apoptotic process GO:0042981 IDA
    regulation of apoptotic process GO:0042981 IMP
    regulation of neuron apoptotic process GO:0043523 IDA
    protein neddylation GO:0045116 IDA
    protein neddylation GO:0045116 IEA
    protein neddylation GO:0045116 IMP
    neuron apoptotic process GO:0051402 IDA
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    protein-containing complex GO:0032991 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified NAE1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NAE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 UBE2F  
Biochemical Activity Homo sapiens
4 NUCB2 4925
Co-fractionation Homo sapiens
5 ZNF684  
Affinity Capture-MS Homo sapiens
6 NSFL1C 55968
Co-fractionation Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 TP53BP2  
Affinity Capture-Western Homo sapiens
9 RAP1GDS1 5910
Co-fractionation Homo sapiens
10 CSDE1 7812
Co-fractionation Homo sapiens
11 UBQLN4 56893
Two-hybrid Homo sapiens
12 UIMC1  
Affinity Capture-MS Homo sapiens
13 SREBF2 6721
Two-hybrid Homo sapiens
14 UBA5 79876
Co-fractionation Homo sapiens
15 PRNP 5621
Affinity Capture-MS Homo sapiens
16 XPO1 7514
Co-fractionation Homo sapiens
17 LMNB1 4001
Co-fractionation Homo sapiens
18 SAR1B 51128
Affinity Capture-MS Homo sapiens
19 PDIA4 9601
Co-fractionation Homo sapiens
20 UBE2M 9040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
21 CAMK1D 57118
Co-fractionation Homo sapiens
22 RIPPLY1  
Affinity Capture-MS Homo sapiens
23 TGS1  
Affinity Capture-MS Homo sapiens
24 RNF144A  
Affinity Capture-MS Homo sapiens
25 NUDCD3 23386
Co-fractionation Homo sapiens
26 RPS12 6206
Co-fractionation Homo sapiens
27 MCM5 4174
Co-fractionation Homo sapiens
28 SQSTM1 8878
Proximity Label-MS Homo sapiens
29 TXNRD1 7296
Co-fractionation Homo sapiens
30 EGFR 1956
Negative Genetic Homo sapiens
31 DCPS 28960
Co-fractionation Homo sapiens
32 PLOD1 5351
Co-fractionation Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 ANKRD39  
Affinity Capture-MS Homo sapiens
35 PCYT2 5833
Co-fractionation Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
37 WIF1 11197
Affinity Capture-MS Homo sapiens
38 TRIP12 9320
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
39 TUFM 7284
Co-fractionation Homo sapiens
40 APEX1 328
Co-fractionation Homo sapiens
41 PAFAH1B2 5049
Co-fractionation Homo sapiens
42 XRCC6 2547
Co-fractionation Homo sapiens
43 CEBPA  
Protein-peptide Homo sapiens
44 RPS6KB1 6198
Co-fractionation Homo sapiens
45 LRTM1  
Affinity Capture-MS Homo sapiens
46 ALG8  
Two-hybrid Homo sapiens
47 PPP1R2 5504
Co-fractionation Homo sapiens
48 UBA3 9039
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
49 RPA2 6118
Proximity Label-MS Homo sapiens
50 DDX5 1655
Co-fractionation Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 PAPSS1 9061
Co-fractionation Homo sapiens
53 PINK1  
Affinity Capture-MS Homo sapiens
54 LIPA 3988
Co-fractionation Homo sapiens
55 EXOC6 54536
Co-fractionation Homo sapiens
56 LIG1 3978
Co-fractionation Homo sapiens
57 SERBP1 26135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RPA4  
Proximity Label-MS Homo sapiens
59 KRAS 3845
Negative Genetic Homo sapiens
60 MCTS1 28985
Co-fractionation Homo sapiens
61 C9orf72  
Affinity Capture-MS Homo sapiens
62 TMEM183A  
Affinity Capture-MS Homo sapiens
63 OGT 8473
Reconstituted Complex Homo sapiens
64 PFN2 5217
Co-fractionation Homo sapiens
65 DIAPH1 1729
Co-fractionation Homo sapiens
66 MSN 4478
Co-fractionation Homo sapiens
67 RPA3 6119
Proximity Label-MS Homo sapiens
68 CAPN2 824
Co-fractionation Homo sapiens
69 NTNG1  
Affinity Capture-MS Homo sapiens
70 NEDD8 4738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
71 ATIC 471
Co-fractionation Homo sapiens
72 TATDN1 83940
Co-fractionation Homo sapiens
73 PROSC 11212
Co-fractionation Homo sapiens
74 ZBTB44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NAE1 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Neddylation TAS Reactome
Post-translational protein modification TAS Reactome





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