Gene description for LIPA
Gene name lipase A, lysosomal acid, cholesterol esterase
Gene symbol LIPA
Other names/aliases CESD
LAL
Species Homo sapiens
 Database cross references - LIPA
ExoCarta ExoCarta_3988
Vesiclepedia VP_3988
Entrez Gene 3988
HGNC 6617
MIM 613497
UniProt P38571  
 LIPA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LIPA
Molecular Function
    sterol ester esterase activity GO:0004771 IBA
    sterol ester esterase activity GO:0004771 IDA
    sterol ester esterase activity GO:0004771 IMP
    sterol ester esterase activity GO:0004771 TAS
    lipase activity GO:0016298 IDA
Biological Process
    mitotic cell cycle GO:0000278 IEA
    cell morphogenesis GO:0000902 IEA
    endothelial cell proliferation GO:0001935 IEA
    response to dietary excess GO:0002021 IEA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    acute inflammatory response GO:0002526 IEA
    respiratory burst involved in inflammatory response GO:0002536 IEA
    glucose metabolic process GO:0006006 IEA
    glycolytic process GO:0006096 IEA
    fatty acid metabolic process GO:0006631 IEA
    triglyceride metabolic process GO:0006641 IEA
    cholesterol biosynthetic process GO:0006695 IEA
    ATP biosynthetic process GO:0006754 IEA
    vitamin A metabolic process GO:0006776 IEA
    endocytosis GO:0006897 IEA
    mitochondrion organization GO:0007005 IEA
    lysosome organization GO:0007040 IEA
    small GTPase-mediated signal transduction GO:0007264 IEA
    endosome to lysosome transport GO:0008333 IEA
    determination of adult lifespan GO:0008340 IEA
    response to cold GO:0009409 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    gene expression GO:0010467 IEA
    cholesterol storage GO:0010878 IEA
    lipid catabolic process GO:0016042 IEA
    sterol metabolic process GO:0016125 IBA
    myeloid cell differentiation GO:0030099 IEA
    T cell differentiation GO:0030217 IEA
    lung development GO:0030324 IEA
    defecation GO:0030421 IEA
    TOR signaling GO:0031929 IEA
    myeloid cell apoptotic process GO:0033028 IEA
    response to vitamin A GO:0033189 IEA
    cholesterol efflux GO:0033344 IEA
    low-density lipoprotein particle clearance GO:0034383 TAS
    common myeloid progenitor cell proliferation GO:0035726 IEA
    T cell proliferation GO:0042098 IEA
    lipoprotein catabolic process GO:0042159 IEA
    spleen development GO:0048536 IEA
    bone marrow development GO:0048539 IEA
    tissue remodeling GO:0048771 IEA
    homeostasis of number of cells within a tissue GO:0048873 IEA
    positive regulation of T cell receptor signaling pathway GO:0050862 IEA
    regulation of mitochondrial membrane potential GO:0051881 IEA
    lipid homeostasis GO:0055088 IEA
    adipose tissue development GO:0060612 IEA
    blood vessel endothelial cell differentiation GO:0060837 IEA
    macrophage homeostasis GO:0061519 IEA
    T cell apoptotic process GO:0070231 IEA
    fat cell proliferation GO:0070341 IEA
    triglyceride-rich lipoprotein particle clearance GO:0071830 IEA
    cell proliferation in bone marrow GO:0071838 IEA
    liver morphogenesis GO:0072576 IEA
    lipid import into cell GO:0140354 IEA
    multicellular organismal-level chemical homeostasis GO:0140962 IEA
    response to rapamycin GO:1901355 IEA
    reactive oxygen species biosynthetic process GO:1903409 IEA
    adaptive thermogenesis GO:1990845 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 IDA
    lysosome GO:0005764 ISS
    cytosol GO:0005829 IDA
    lysosomal lumen GO:0043202 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified LIPA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LIPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CANX 821
Affinity Capture-MS Homo sapiens
2 MCM3 4172
Co-fractionation Homo sapiens
3 MECP2 4204
Affinity Capture-MS Homo sapiens
4 PRG2  
Affinity Capture-MS Homo sapiens
5 MRPL21 219927
Affinity Capture-MS Homo sapiens
6 HBS1L 10767
Affinity Capture-MS Homo sapiens
7 JUP 3728
Co-fractionation Homo sapiens
8 GTF2I 2969
Co-fractionation Homo sapiens
9 GARS 2617
Cross-Linking-MS (XL-MS) Homo sapiens
10 PPM1B 5495
Affinity Capture-MS Homo sapiens
11 RPA2 6118
Proximity Label-MS Homo sapiens
12 KIAA1217 56243
Co-fractionation Homo sapiens
13 MEX3A  
Affinity Capture-RNA Homo sapiens
14 NAA15 80155
Co-fractionation Homo sapiens
15 NAE1 8883
Co-fractionation Homo sapiens
16 BCAP31 10134
Affinity Capture-MS Homo sapiens
17 CBLN4  
Affinity Capture-MS Homo sapiens
18 CAPZA1 829
Co-fractionation Homo sapiens
19 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
20 FBXO6 26270
Affinity Capture-MS Homo sapiens
21 NCR3  
Affinity Capture-MS Homo sapiens
22 RAD50 10111
Co-fractionation Homo sapiens
23 SSH3 54961
Co-fractionation Homo sapiens
24 PCNA 5111
Co-fractionation Homo sapiens
25 TMEM154  
Affinity Capture-MS Homo sapiens
26 CDH1 999
Co-fractionation Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 NIN 51199
Proximity Label-MS Homo sapiens
29 FBXO2 26232
Affinity Capture-MS Homo sapiens
30 STIM2 57620
Affinity Capture-MS Homo sapiens
31 IGF2R 3482
Affinity Capture-MS Homo sapiens
32 EIF5A 1984
Co-fractionation Homo sapiens
33 TTC9C 283237
Affinity Capture-MS Homo sapiens
34 TPP2 7174
Cross-Linking-MS (XL-MS) Homo sapiens
35 ITPK1 3705
Co-fractionation Homo sapiens
36 CLGN 1047
Affinity Capture-MS Homo sapiens
37 GAL3ST2  
Affinity Capture-MS Homo sapiens
38 TCF25 22980
Affinity Capture-MS Homo sapiens
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