Gene description for GTF2I
Gene name general transcription factor IIi
Gene symbol GTF2I
Other names/aliases BAP135
BTKAP1
DIWS
GTFII-I
IB291
SPIN
TFII-I
WBS
WBSCR6
Species Homo sapiens
 Database cross references - GTF2I
ExoCarta ExoCarta_2969
Vesiclepedia VP_2969
Entrez Gene 2969
HGNC 4659
MIM 601679
UniProt P78347  
 GTF2I identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for GTF2I
Molecular Function
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 NAS
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA binding GO:0003677 IEA
    DNA-binding transcription factor activity GO:0003700 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
Biological Process
    transcription by RNA polymerase II GO:0006366 IEA
    negative regulation of angiogenesis GO:0016525 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IEA
    membrane GO:0016020 HDA
 Experiment description of studies that identified GTF2I in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 234
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
16
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
17
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
20
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTF2I
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 MCRS1  
Affinity Capture-MS Homo sapiens
3 LHFPL4  
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Co-fractionation Homo sapiens
5 AP2B1 163
Two-hybrid Homo sapiens
6 TRMT1L 81627
Co-fractionation Homo sapiens
7 PRDM1  
Affinity Capture-MS Homo sapiens
8 HDAC2 3066
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CCT3 7203
Co-fractionation Homo sapiens
10 MAGEA3  
Affinity Capture-MS Homo sapiens
11 CTPS2 56474
Co-fractionation Homo sapiens
12 GJA1 2697
Affinity Capture-MS Homo sapiens
13 PSMA4 5685
Co-fractionation Homo sapiens
14 NFKB1 4790
Co-fractionation Homo sapiens
15 Pias2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
16 RPA2 6118
Affinity Capture-MS Homo sapiens
17 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
18 ACTC1 70
Proximity Label-MS Homo sapiens
19 BRCA1 672
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
20 MAGEA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LGALS1 3956
Affinity Capture-Western Homo sapiens
22 TIGD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 C4BPA 722
Two-hybrid Homo sapiens
24 UBC 7316
Reconstituted Complex Homo sapiens
25 CALM1 801
Reconstituted Complex Homo sapiens
26 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
27 RPS6KB2  
Affinity Capture-MS Homo sapiens
28 PRKDC 5591
Co-fractionation Homo sapiens
29 FNTA 2339
Co-fractionation Homo sapiens
30 TRIM54  
Two-hybrid Homo sapiens
31 CENPA  
Proximity Label-MS Homo sapiens
32 USP36  
Affinity Capture-MS Homo sapiens
33 KIAA1429 25962
Affinity Capture-MS Homo sapiens
34 CAPZB 832
Affinity Capture-MS Homo sapiens
35 HDAC3 8841
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 WDR76  
Affinity Capture-MS Homo sapiens
37 RDX 5962
Co-fractionation Homo sapiens
38 PCNT  
Cross-Linking-MS (XL-MS) Homo sapiens
39 SUV420H2  
Affinity Capture-MS Homo sapiens
40 PRDX3 10935
Co-fractionation Homo sapiens
41 MTUS2 23281
Two-hybrid Homo sapiens
42 CHAF1A  
Affinity Capture-MS Homo sapiens
43 KLF8  
Affinity Capture-MS Homo sapiens
44 RFXANK  
Affinity Capture-MS Homo sapiens
45 IMPDH2 3615
Co-fractionation Homo sapiens
46 STX7 8417
Affinity Capture-MS Homo sapiens
47 SRRM2 23524
Affinity Capture-MS Homo sapiens
48 OTUB2  
Affinity Capture-MS Homo sapiens
49 KLF15  
Affinity Capture-MS Homo sapiens
50 MAD2L2 10459
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SOST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 TRAF6 7189
Two-hybrid Homo sapiens
53 NFATC2IP  
Co-fractionation Homo sapiens
54 KPNA4 3840
Affinity Capture-MS Homo sapiens
55 TULP3 7289
Affinity Capture-MS Homo sapiens
56 COMT 1312
Co-fractionation Homo sapiens
57 EED  
Affinity Capture-MS Homo sapiens
58 UCHL5 51377
Affinity Capture-MS Homo sapiens
59 DDX6 1656
Co-fractionation Homo sapiens
60 DFFA 1676
Co-fractionation Homo sapiens
61 PFN2 5217
Co-fractionation Homo sapiens
62 TFCP2 7024
Affinity Capture-MS Homo sapiens
63 MYCN  
Affinity Capture-MS Homo sapiens
64 USP7 7874
Affinity Capture-MS Homo sapiens
65 SLU7  
Reconstituted Complex Homo sapiens
66 NHLH2  
Affinity Capture-MS Homo sapiens
67 EEF1A1 1915
Co-fractionation Homo sapiens
68 Lima1  
Affinity Capture-MS Mus musculus
69 USP37  
Proximity Label-MS Homo sapiens
70 KIF14 9928
Affinity Capture-MS Homo sapiens
71 ESR1  
Affinity Capture-MS Homo sapiens
72 RBM25 58517
Cross-Linking-MS (XL-MS) Homo sapiens
73 ADSL 158
Co-fractionation Homo sapiens
74 TIPRL 261726
Co-fractionation Homo sapiens
75 CCT2 10576
Co-fractionation Homo sapiens
76 KLF16  
Affinity Capture-MS Homo sapiens
77 CHMP4C 92421
Affinity Capture-MS Homo sapiens
78 INO80E  
Affinity Capture-MS Homo sapiens
79 KLF5  
Affinity Capture-MS Homo sapiens
80 MAP3K3 4215
Two-hybrid Homo sapiens
81 RAD23B 5887
Co-fractionation Homo sapiens
82 TCP1 6950
Co-fractionation Homo sapiens
83 FOXC1  
Affinity Capture-MS Homo sapiens
84 MAPK3 5595
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
85 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
86 NFRKB  
Affinity Capture-MS Homo sapiens
87 BCL2L11  
Reconstituted Complex Homo sapiens
88 MAPK12  
Two-hybrid Homo sapiens
89 MEPCE 56257
Affinity Capture-MS Homo sapiens
90 WHSC1 7468
Proximity Label-MS Homo sapiens
91 CDR2L  
Affinity Capture-MS Homo sapiens
92 PRPS2 5634
Co-fractionation Homo sapiens
93 TUFM 7284
Co-fractionation Homo sapiens
94 ZMYM3  
Two-hybrid Homo sapiens
95 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
96 RPS6KB1 6198
Co-fractionation Homo sapiens
97 OTUD1 220213
Affinity Capture-MS Homo sapiens
98 UBE2I 7329
Biochemical Activity Homo sapiens
99 PTP4A3  
Affinity Capture-MS Homo sapiens
100 CHMP4B 128866
Affinity Capture-MS Homo sapiens
101 GCC1 79571
Two-hybrid Homo sapiens
102 MTMR7  
Affinity Capture-MS Homo sapiens
103 DDX5 1655
Co-fractionation Homo sapiens
104 NTRK1 4914
Affinity Capture-MS Homo sapiens
105 AIM2  
Affinity Capture-MS Homo sapiens
106 PIAS2  
Two-hybrid Homo sapiens
107 TAB2  
Two-hybrid Homo sapiens
108 SRF  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
109 SMAD2 4087
Affinity Capture-Western Homo sapiens
110 GTF2IRD2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 Mad2l2  
Affinity Capture-MS Mus musculus
112 BTK 695
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
113 YAP1 10413
Affinity Capture-MS Homo sapiens
114 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
115 METTL1 4234
Co-fractionation Homo sapiens
116 VPS11 55823
Affinity Capture-MS Homo sapiens
117 PTAR1 375743
Synthetic Lethality Homo sapiens
118 CTAG1A  
Two-hybrid Homo sapiens
119 ANLN 54443
Affinity Capture-MS Homo sapiens
120 TRIM21 6737
Affinity Capture-MS Homo sapiens
121 PHLPP1  
Proximity Label-MS Homo sapiens
122 SLX4  
Affinity Capture-MS Homo sapiens
123 LSM8 51691
Two-hybrid Homo sapiens
124 AHCY 191
Co-fractionation Homo sapiens
125 FANCD2  
Affinity Capture-MS Homo sapiens
126 TFAP4 7023
Affinity Capture-MS Homo sapiens
127 GTF2IRD2  
Affinity Capture-MS Homo sapiens
128 C9orf78 51759
Affinity Capture-MS Homo sapiens
129 CBX3 11335
Proximity Label-MS Homo sapiens
130 ECT2 1894
Affinity Capture-MS Homo sapiens
131 DNMT3L  
Reconstituted Complex Homo sapiens
132 SIRT7  
Affinity Capture-MS Homo sapiens
133 USP9X 8239
Cross-Linking-MS (XL-MS) Homo sapiens
134 PRRX1 5396
Co-purification Homo sapiens
135 RLIM 51132
Affinity Capture-MS Homo sapiens
136 NFKBIA  
Co-fractionation Homo sapiens
137 PRKAR1A 5573
Co-fractionation Homo sapiens
138 HSP90AB1 3326
Co-fractionation Homo sapiens
139 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 BTF3 689
Affinity Capture-MS Homo sapiens
141 KIAA1598 57698
Co-fractionation Homo sapiens
142 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 YY1 7528
Affinity Capture-MS Homo sapiens
144 TFPT  
Affinity Capture-MS Homo sapiens
145 FBXO6 26270
Affinity Capture-MS Homo sapiens
146 METTL21B  
Affinity Capture-MS Homo sapiens
147 POLR2A 5430
Affinity Capture-Western Homo sapiens
148 SUZ12  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
149 TIMM50 92609
Affinity Capture-MS Homo sapiens
150 Espl1  
Affinity Capture-MS Mus musculus
151 FBXW11  
Affinity Capture-MS Homo sapiens
152 STAT1 6772
Reconstituted Complex Homo sapiens
153 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
154 MYC  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
155 TOPORS  
Affinity Capture-MS Homo sapiens
156 NUCB1 4924
Co-fractionation Homo sapiens
157 FDPS 2224
Co-fractionation Homo sapiens
158 ATF6  
Affinity Capture-Western Homo sapiens
159 WDR5 11091
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
160 INO80  
Affinity Capture-MS Homo sapiens
161 PHF21A  
Affinity Capture-Western Homo sapiens
162 NDC80 10403
Co-fractionation Homo sapiens
163 RNF2  
Affinity Capture-MS Homo sapiens
164 STYX  
Affinity Capture-MS Homo sapiens
165 LMNA 4000
Co-fractionation Homo sapiens
166 RIPK4  
Affinity Capture-MS Homo sapiens
167 PGPEP1 54858
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
168 MDM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
169 NAA10 8260
Co-fractionation Homo sapiens
170 DSP 1832
Co-fractionation Homo sapiens
171 HIF1A 3091
Affinity Capture-MS Homo sapiens
172 CDC25A  
Affinity Capture-MS Homo sapiens
173 MRPL42  
Affinity Capture-MS Homo sapiens
174 EIF3F 8665
Affinity Capture-MS Homo sapiens
175 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
176 ZNF428  
Affinity Capture-MS Homo sapiens
177 RARA 5914
Affinity Capture-MS Homo sapiens
178 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 AP4S1 11154
Two-hybrid Homo sapiens
180 SRRM1 10250
Affinity Capture-MS Homo sapiens
181 TP53 7157
Affinity Capture-MS Homo sapiens
182 TUBG1 7283
Affinity Capture-MS Homo sapiens
183 INPPL1 3636
Affinity Capture-MS Homo sapiens
184 XRCC6 2547
Co-fractionation Homo sapiens
185 DDX3X 1654
Co-fractionation Homo sapiens
186 CTAG1B  
Two-hybrid Homo sapiens
187 POU2AF1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
188 RPA1 6117
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
189 DPY30 84661
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
190 PDHA1 5160
Co-fractionation Homo sapiens
191 TPD52L2 7165
Co-fractionation Homo sapiens
192 PRMT1 3276
Affinity Capture-MS Homo sapiens
193 HDAC1 3065
Affinity Capture-Western Homo sapiens
194 CDK2 1017
Affinity Capture-MS Homo sapiens
195 FASN 2194
Negative Genetic Homo sapiens
196 DDX58 23586
Affinity Capture-RNA Homo sapiens
197 ACTR8 93973
Affinity Capture-MS Homo sapiens
198 MTA2 9219
Co-fractionation Homo sapiens
199 MAPK1 5594
Affinity Capture-Western Homo sapiens
200 Tuba3a 22144
Affinity Capture-MS Mus musculus
201 TLR9  
Affinity Capture-MS Homo sapiens
202 UFL1 23376
Affinity Capture-MS Homo sapiens
203 SNRPN 6638
Reconstituted Complex Homo sapiens
204 KDM1A 23028
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
205 EZH2  
Affinity Capture-MS Homo sapiens
206 HNRNPL 3191
Co-fractionation Homo sapiens
207 SRSF9 8683
Co-fractionation Homo sapiens
208 HMGA1 3159
Cross-Linking-MS (XL-MS) Homo sapiens
209 EIF4B 1975
Co-fractionation Homo sapiens
210 STAT3 6774
Reconstituted Complex Homo sapiens
211 PHACTR3  
Affinity Capture-MS Homo sapiens
212 LIPA 3988
Co-fractionation Homo sapiens
213 METTL3  
Affinity Capture-MS Homo sapiens
214 RPL28 6158
Co-fractionation Homo sapiens
215 ZUFSP 221302
Affinity Capture-MS Homo sapiens
216 HIRA  
Affinity Capture-Western Homo sapiens
217 C9orf72  
Affinity Capture-MS Homo sapiens
218 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
219 USF1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
220 ATXN3 4287
Affinity Capture-MS Homo sapiens
221 WARS 7453
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
222 JAK2 3717
Biochemical Activity Homo sapiens
223 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
224 USP32 84669
Affinity Capture-MS Homo sapiens
225 ZNF217 7764
Co-fractionation Homo sapiens
226 EP300 2033
Affinity Capture-MS Homo sapiens
227 PRKG1 5592
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2I is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here