Gene description for PRRX1
Gene name paired related homeobox 1
Gene symbol PRRX1
Other names/aliases AGOTC
PHOX1
PMX1
PRX-1
PRX1
Species Homo sapiens
 Database cross references - PRRX1
ExoCarta ExoCarta_5396
Vesiclepedia VP_5396
Entrez Gene 5396
HGNC 9142
MIM 167420
UniProt P54821  
 PRRX1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PRRX1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IEA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    HMG box domain binding GO:0071837 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 IEA
    smoothened signaling pathway GO:0007224 IEA
    mesenchymal cell proliferation GO:0010463 IEA
    embryonic limb morphogenesis GO:0030326 IEA
    inner ear morphogenesis GO:0042472 IEA
    middle ear morphogenesis GO:0042474 IEA
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    neuron fate determination GO:0048664 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    artery morphogenesis GO:0048844 IEA
    cartilage development GO:0051216 IEA
    roof of mouth development GO:0060021 IEA
    regulation of neuron projection regeneration GO:0070570 IEA
    stem cell proliferation GO:0072089 IEA
    neuronal stem cell population maintenance GO:0097150 IEA
    positive regulation of stem cell proliferation GO:2000648 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified PRRX1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRRX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNMT3A 1788
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 SAAL1 113174
Two-hybrid Homo sapiens
3 MYC  
Affinity Capture-MS Homo sapiens
4 CINP  
Two-hybrid Homo sapiens
5 GTF2I 2969
Co-purification Homo sapiens
6 ALX3  
Affinity Capture-MS Homo sapiens
7 FKBP5 2289
Affinity Capture-MS Homo sapiens
8 SUOX  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PRRX1 is involved
No pathways found





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