Gene description for ITPK1
Gene name inositol-tetrakisphosphate 1-kinase
Gene symbol ITPK1
Other names/aliases ITRPK1
Species Homo sapiens
 Database cross references - ITPK1
ExoCarta ExoCarta_3705
Vesiclepedia VP_3705
Entrez Gene 3705
HGNC 6177
MIM 601838
UniProt Q13572  
 ITPK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ITPK1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    inositol-1,3,4,5-tetrakisphosphate 6-kinase activity GO:0000825 IMP
    catalytic activity GO:0003824 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    isomerase activity GO:0016853 IEA
    inositol-3,4,5,6-tetrakisphosphate 1-kinase activity GO:0047325 EXP
    inositol-3,4,5,6-tetrakisphosphate 1-kinase activity GO:0047325 IBA
    inositol-1,3,4-trisphosphate 6-kinase activity GO:0052725 EXP
    inositol-1,3,4-trisphosphate 6-kinase activity GO:0052725 IBA
    inositol-1,3,4-trisphosphate 5-kinase activity GO:0052726 EXP
    inositol-1,3,4-trisphosphate 5-kinase activity GO:0052726 IBA
    inositol-3,4,6-trisphosphate 1-kinase activity GO:0052835 IEA
Biological Process
    signal transduction GO:0007165 TAS
    blood coagulation GO:0007596 TAS
    neural tube development GO:0021915 IEA
    inositol trisphosphate metabolic process GO:0032957 IEA
    necroptotic process GO:0070266 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    apical plasma membrane GO:0016324 IEA
 Experiment description of studies that identified ITPK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ITPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 GPS1 2873
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 TRAF2 7186
Two-hybrid Homo sapiens
5 DNAAF5 54919
Co-fractionation Homo sapiens
6 TP53 7157
Biochemical Activity Homo sapiens
7 PRNP 5621
Affinity Capture-MS Homo sapiens
8 MAPK1 5594
Co-fractionation Homo sapiens
9 CRYZL1 9946
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NFKBIA  
Biochemical Activity Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 CCT6B 10693
Affinity Capture-MS Homo sapiens
13 INPP4B 8821
Co-fractionation Homo sapiens
14 JUN 3725
Biochemical Activity Homo sapiens
15 MOV10 4343
Affinity Capture-RNA Homo sapiens
16 LIPA 3988
Co-fractionation Homo sapiens
17 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TRAC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ITPK1 3705
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
20 P2RY10  
Affinity Capture-MS Homo sapiens
21 TACSTD2 4070
Affinity Capture-MS Homo sapiens
22 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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