Gene description for DNAAF5
Gene name dynein, axonemal, assembly factor 5
Gene symbol DNAAF5
Other names/aliases CILD18
HEATR2
Species Homo sapiens
 Database cross references - DNAAF5
ExoCarta ExoCarta_54919
Vesiclepedia VP_54919
Entrez Gene 54919
HGNC 26013
MIM 614864
UniProt Q86Y56  
 DNAAF5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for DNAAF5
Molecular Function
    dynein intermediate chain binding GO:0045505 IBA
    dynein intermediate chain binding GO:0045505 IPI
Biological Process
    cilium movement GO:0003341 IBA
    cilium movement GO:0003341 IMP
    outer dynein arm assembly GO:0036158 IBA
    outer dynein arm assembly GO:0036158 IMP
    inner dynein arm assembly GO:0036159 IBA
    inner dynein arm assembly GO:0036159 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    motile cilium GO:0031514 IDA
    dynein axonemal particle GO:0120293 ISS
 Experiment description of studies that identified DNAAF5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNAAF5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PTPRF 5792
Co-fractionation Homo sapiens
3 LYPD3 27076
Affinity Capture-MS Homo sapiens
4 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NPLOC4 55666
Co-fractionation Homo sapiens
6 D2HGDH 728294
Affinity Capture-MS Homo sapiens
7 CMTM8  
Affinity Capture-MS Homo sapiens
8 HCST  
Affinity Capture-MS Homo sapiens
9 TRIM35 23087
Affinity Capture-MS Homo sapiens
10 FPR1  
Affinity Capture-MS Homo sapiens
11 ADRBK1 156
Co-fractionation Homo sapiens
12 DPEP1 1800
Affinity Capture-MS Homo sapiens
13 ASXL1  
Affinity Capture-MS Homo sapiens
14 SLC18A2  
Affinity Capture-MS Homo sapiens
15 PPP2R1B 5519
Co-fractionation Homo sapiens
16 SLC15A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SCARA3  
Affinity Capture-MS Homo sapiens
18 GFPT1 2673
Co-fractionation Homo sapiens
19 PPP2R5D 5528
Co-fractionation Homo sapiens
20 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
21 RFXANK  
Affinity Capture-MS Homo sapiens
22 SLCO1B1  
Affinity Capture-MS Homo sapiens
23 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 C5AR1  
Affinity Capture-MS Homo sapiens
25 SEMA4C 54910
Affinity Capture-MS Homo sapiens
26 CXCR4 7852
Affinity Capture-MS Homo sapiens
27 P2RY10  
Affinity Capture-MS Homo sapiens
28 KLK15  
Affinity Capture-MS Homo sapiens
29 ITFG3 83986
Affinity Capture-MS Homo sapiens
30 HYPM  
Affinity Capture-MS Homo sapiens
31 POLR2C 5432
Proximity Label-MS Homo sapiens
32 BRF2  
Affinity Capture-MS Homo sapiens
33 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NPAS1  
Affinity Capture-MS Homo sapiens
35 FKBP10 60681
Co-fractionation Homo sapiens
36 GPR17 2840
Affinity Capture-MS Homo sapiens
37 MAPK1 5594
Co-fractionation Homo sapiens
38 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CD70 970
Affinity Capture-MS Homo sapiens
40 RAD23B 5887
Co-fractionation Homo sapiens
41 PLBD1 79887
Affinity Capture-MS Homo sapiens
42 C19orf38  
Affinity Capture-MS Homo sapiens
43 ACTR3 10096
Co-fractionation Homo sapiens
44 ZBTB16  
Two-hybrid Homo sapiens
45 PNKP 11284
Affinity Capture-MS Homo sapiens
46 STOM 2040
Affinity Capture-MS Homo sapiens
47 P2RY8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PTP4A3  
Affinity Capture-MS Homo sapiens
50 CHMP4B 128866
Affinity Capture-MS Homo sapiens
51 C16orf58 64755
Affinity Capture-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 GPR182  
Affinity Capture-MS Homo sapiens
54 PLK1 5347
Affinity Capture-MS Homo sapiens
55 TSC22D1 8848
Affinity Capture-MS Homo sapiens
56 ARMC1 55156
Co-fractionation Homo sapiens
57 TMCO3 55002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 METTL1 4234
Co-fractionation Homo sapiens
59 UBXN6 80700
Affinity Capture-MS Homo sapiens
60 USP32 84669
Affinity Capture-MS Homo sapiens
61 KDF1  
Affinity Capture-MS Homo sapiens
62 CASP4  
Affinity Capture-MS Homo sapiens
63 PSG11  
Affinity Capture-MS Homo sapiens
64 ZBED6CL  
Affinity Capture-MS Homo sapiens
65 NT5E 4907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CD40 958
Affinity Capture-MS Homo sapiens
67 Rab5c 19345
Affinity Capture-MS Mus musculus
68 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CYB5B 80777
Affinity Capture-MS Homo sapiens
70 EFNA4  
Affinity Capture-MS Homo sapiens
71 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CSTF1 1477
Co-fractionation Homo sapiens
73 CIAO1 9391
Affinity Capture-MS Homo sapiens
74 FZD10  
Affinity Capture-MS Homo sapiens
75 NPTN 27020
Affinity Capture-MS Homo sapiens
76 CMKLR1 1240
Affinity Capture-MS Homo sapiens
77 CUL3 8452
Affinity Capture-MS Homo sapiens
78 NPY2R  
Affinity Capture-MS Homo sapiens
79 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 AQP3  
Affinity Capture-MS Homo sapiens
81 NXF1 10482
Affinity Capture-RNA Homo sapiens
82 Espl1  
Affinity Capture-MS Mus musculus
83 RRM2 6241
Co-fractionation Homo sapiens
84 C12orf74  
Affinity Capture-MS Homo sapiens
85 DCUN1D1 54165
Co-fractionation Homo sapiens
86 C3orf18  
Affinity Capture-MS Homo sapiens
87 PPP2R1A 5518
Co-fractionation Homo sapiens
88 DNAI2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
89 ARL4D  
Affinity Capture-MS Homo sapiens
90 GPR45  
Affinity Capture-MS Homo sapiens
91 GSTK1 373156
Affinity Capture-MS Homo sapiens
92 METTL21B  
Affinity Capture-MS Homo sapiens
93 PTPRN  
Affinity Capture-MS Homo sapiens
94 MAGEA6  
Affinity Capture-MS Homo sapiens
95 DDRGK1 65992
Affinity Capture-MS Homo sapiens
96 B3GAT3 26229
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 ITPK1 3705
Co-fractionation Homo sapiens
98 ILK 3611
Affinity Capture-MS Homo sapiens
99 FSCN1 6624
Affinity Capture-MS Homo sapiens
100 LMNA 4000
Proximity Label-MS Homo sapiens
101 AVPR2  
Affinity Capture-MS Homo sapiens
102 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 GYPA  
Affinity Capture-MS Homo sapiens
104 FEZ1  
Two-hybrid Homo sapiens
105 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
106 HSPB8 26353
Affinity Capture-MS Homo sapiens
107 Bap1  
Affinity Capture-MS Mus musculus
108 BKRF1  
Affinity Capture-MS
109 ILF3 3609
Affinity Capture-RNA Homo sapiens
110 XPO6 23214
Co-fractionation Homo sapiens
111 PGRMC1 10857
Affinity Capture-MS Homo sapiens
112 LDLRAD1  
Affinity Capture-MS Homo sapiens
113 PHLDA3 23612
Affinity Capture-MS Homo sapiens
114 KCTD14 65987
Affinity Capture-MS Homo sapiens
115 Cep135  
Affinity Capture-MS Mus musculus
116 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 EFNB1 1947
Affinity Capture-MS Homo sapiens
118 MFSD4  
Affinity Capture-MS Homo sapiens
119 GPRC5D  
Affinity Capture-MS Homo sapiens
120 CD80 941
Affinity Capture-MS Homo sapiens
121 GSTM3 2947
Affinity Capture-MS Homo sapiens
122 PLD3 23646
Affinity Capture-MS Homo sapiens
123 MAGED1 9500
Affinity Capture-MS Homo sapiens
124 MOV10 4343
Affinity Capture-RNA Homo sapiens
125 EZH2  
Affinity Capture-MS Homo sapiens
126 FLNA 2316
Co-fractionation Homo sapiens
127 HNRNPF 3185
Co-fractionation Homo sapiens
128 SERBP1 26135
Affinity Capture-MS Homo sapiens
129 MAS1  
Affinity Capture-MS Homo sapiens
130 FAM96A  
Affinity Capture-MS Homo sapiens
131 FXYD3 5349
Affinity Capture-MS Homo sapiens
132 RPA3 6119
Proximity Label-MS Homo sapiens
133 FPR2  
Affinity Capture-MS Homo sapiens
134 CXCL16 58191
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAAF5 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here