Gene description for Top2b
Gene name topoisomerase (DNA) II beta
Gene symbol Top2b
Other names/aliases D230016L12Rik
Top-2
Species Mus musculus
 Database cross references - Top2b
ExoCarta ExoCarta_21974
Vesiclepedia VP_21974
Entrez Gene 21974
UniProt Q64511  
 Top2b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Top2b
Molecular Function
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 IEA
    chromatin binding GO:0003682 ISO
    DNA topoisomerase activity GO:0003916 ISS
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 IMP
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 ISO
    DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity GO:0003918 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    DNA negative supercoiling activity GO:0034335 IEA
    ribonucleoprotein complex binding GO:0043021 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    resolution of meiotic recombination intermediates GO:0000712 IBA
    sister chromatid segregation GO:0000819 IBA
    neuron migration GO:0001764 IMP
    DNA topological change GO:0006265 ISO
    DNA topological change GO:0006265 ISS
    axonogenesis GO:0007409 IMP
    B cell differentiation GO:0030183 IMP
    B cell differentiation GO:0030183 ISO
    forebrain development GO:0030900 IMP
    positive regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045870 IEA
    positive regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045870 ISO
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to ATP GO:0071318 IEA
    cellular senescence GO:0090398 IEA
    positive regulation of double-strand break repair via nonhomologous end joining GO:2001034 IEA
Subcellular Localization
    heterochromatin GO:0000792 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 IDA
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Top2b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Top2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ranbp2  
Co-fractionation Mus musculus
2 ITPR1 3708
Affinity Capture-MS Homo sapiens
3 Zbtb7b  
Affinity Capture-MS Mus musculus
4 Elf5  
Affinity Capture-MS Mus musculus
5 Uvrag  
Co-fractionation Mus musculus
6 TOP2A 7153
Affinity Capture-MS Homo sapiens
7 Atg16l1  
Affinity Capture-MS Mus musculus
8 ZNF451 26036
Affinity Capture-MS Homo sapiens
9 Uhrf1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
10 Pou5f1  
Affinity Capture-MS Mus musculus
11 Unk  
Affinity Capture-RNA Mus musculus
12 Tgfbrap1  
Co-fractionation Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
14 TOP2B 7155
Affinity Capture-MS Homo sapiens
15 1700021K19Rik  
Co-fractionation Mus musculus
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