Gene description for HIST2H2BF
Gene name histone cluster 2, H2bf
Gene symbol HIST2H2BF
Other names/aliases -
Species Homo sapiens
 Database cross references - HIST2H2BF
ExoCarta ExoCarta_440689
Vesiclepedia VP_440689
Entrez Gene 440689
HGNC 24700
UniProt Q5QNW6  
 HIST2H2BF identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Urine 19056867    
 Gene ontology annotations for HIST2H2BF
Molecular Function
    DNA binding GO:0003677 IEA
    structural constituent of chromatin GO:0030527 IEA
    protein heterodimerization activity GO:0046982 IEA
Subcellular Localization
    nucleosome GO:0000786 IEA
    nucleus GO:0005634 HDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HIST2H2BF in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
6
Experiment ID 139
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
7
Experiment ID 140
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
8
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
9
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
10
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
11
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
12
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
13
Experiment ID 146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
14
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HIST2H2BF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-MS Homo sapiens
2 HIST1H2AI 8329
Cross-Linking-MS (XL-MS) Homo sapiens
3 HMGN3  
Cross-Linking-MS (XL-MS) Homo sapiens
4 DUOX2 50506
Cross-Linking-MS (XL-MS) Homo sapiens
5 H2AFJ 55766
Cross-Linking-MS (XL-MS) Homo sapiens
6 HIST1H2AA 221613
Cross-Linking-MS (XL-MS) Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
9 TMPO 7112
Affinity Capture-MS Homo sapiens
10 MYCN  
Affinity Capture-MS Homo sapiens
11 TOP2A 7153
Cross-Linking-MS (XL-MS) Homo sapiens
12 SPRTN  
Affinity Capture-MS Homo sapiens
13 HMGN4  
Cross-Linking-MS (XL-MS) Homo sapiens
14 HIST1H3A 8350
Proximity Label-MS Homo sapiens
15 HIST1H2AC 8334
Cross-Linking-MS (XL-MS) Homo sapiens
16 PDS5B 23047
Cross-Linking-MS (XL-MS) Homo sapiens
17 FOLR1 2348
Affinity Capture-MS Homo sapiens
18 CDK5R2 8941
Cross-Linking-MS (XL-MS) Homo sapiens
19 ATP1A3 478
Cross-Linking-MS (XL-MS) Homo sapiens
20 TEX35  
Cross-Linking-MS (XL-MS) Homo sapiens
21 MCM5 4174
Affinity Capture-MS Homo sapiens
22 BTF3 689
Affinity Capture-MS Homo sapiens
23 SMARCC1 6599
Cross-Linking-MS (XL-MS) Homo sapiens
24 HIST2H2AA3 8337
Cross-Linking-MS (XL-MS) Homo sapiens
25 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
26 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
27 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
28 KNOP1 400506
Cross-Linking-MS (XL-MS) Homo sapiens
29 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
30 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
31 SRSF5 6430
Affinity Capture-MS Homo sapiens
32 UBC 7316
Cross-Linking-MS (XL-MS) Homo sapiens
33 U2SURP 23350
Cross-Linking-MS (XL-MS) Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 HMGN2 3151
Cross-Linking-MS (XL-MS) Homo sapiens
36 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
37 ZC3H10  
Affinity Capture-MS Homo sapiens
38 PSMD14 10213
Affinity Capture-MS Homo sapiens
39 UFL1 23376
Affinity Capture-MS Homo sapiens
40 DPPA2  
Cross-Linking-MS (XL-MS) Homo sapiens
41 UBB 7314
Cross-Linking-MS (XL-MS) Homo sapiens
42 MAPK6  
Cross-Linking-MS (XL-MS) Homo sapiens
43 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
44 RPL10A 4736
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 REV3L  
Cross-Linking-MS (XL-MS) Homo sapiens
46 ATAD5  
Cross-Linking-MS (XL-MS) Homo sapiens
47 HIST1H2AH 85235
Cross-Linking-MS (XL-MS) Homo sapiens
48 MYC  
Affinity Capture-MS Homo sapiens
49 PCLO 27445
Cross-Linking-MS (XL-MS) Homo sapiens
50 VCP 7415
Affinity Capture-MS Homo sapiens
51 C16orf72 29035
Affinity Capture-MS Homo sapiens
52 MCM2 4171
Affinity Capture-MS Homo sapiens
53 RPL7A 6130
Affinity Capture-MS Homo sapiens
54 PINK1  
Affinity Capture-MS Homo sapiens
55 ADAMTS12 81792
Cross-Linking-MS (XL-MS) Homo sapiens
56 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
57 TNN 63923
Cross-Linking-MS (XL-MS) Homo sapiens
58 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
59 HIST2H2AB 317772
Cross-Linking-MS (XL-MS) Homo sapiens
60 SERBP1 26135
Affinity Capture-MS Homo sapiens
61 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
62 DDRGK1 65992
Affinity Capture-MS Homo sapiens
63 C9orf72  
Affinity Capture-MS Homo sapiens
64 HECTD1 25831
Affinity Capture-MS Homo sapiens
65 RBM34  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
67 HCLS1 3059
Cross-Linking-MS (XL-MS) Homo sapiens
68 HMGA1 3159
Cross-Linking-MS (XL-MS) Homo sapiens
69 ABCE1 6059
Affinity Capture-MS Homo sapiens
70 CYLC1 1538
Cross-Linking-MS (XL-MS) Homo sapiens
71 ZNF474  
Cross-Linking-MS (XL-MS) Homo sapiens
72 HIST1H2AJ 8331
Cross-Linking-MS (XL-MS) Homo sapiens
73 WDHD1  
Cross-Linking-MS (XL-MS) Homo sapiens
74 NELFE 7936
Affinity Capture-MS Homo sapiens
75 HIST2H2AC 8338
Cross-Linking-MS (XL-MS) Homo sapiens
76 ITGA4 3676
Affinity Capture-MS Homo sapiens
77 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
78 H2AFX 3014
Cross-Linking-MS (XL-MS) Homo sapiens
79 TRO  
Cross-Linking-MS (XL-MS) Homo sapiens
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